Experimental Hybrids of the <i>Triatoma brasiliensis</i> Species Complex Show Higher Susceptibility to the <i>Trypanosoma cruzi</i> Infection Than Their Parentals
The <i>Triatoma brasiliensis</i> species complex is a monophyletic group encompassing two subspecies and six species. Recently, a hybrid zone of members of this complex was recorded in the state of Pernambuco. Questions concerning the capability of the hybrids to become infected with <...
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MDPI AG
2023-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/12/2850 |
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| author | Nathália Correia Letícia Paschoaletto Carolina Reigada Teresa Cristina Monte Gonçalves Carlos José de Carvalho Moreira Jane Costa |
| author_facet | Nathália Correia Letícia Paschoaletto Carolina Reigada Teresa Cristina Monte Gonçalves Carlos José de Carvalho Moreira Jane Costa |
| author_sort | Nathália Correia |
| collection | DOAJ |
| description | The <i>Triatoma brasiliensis</i> species complex is a monophyletic group encompassing two subspecies and six species. Recently, a hybrid zone of members of this complex was recorded in the state of Pernambuco. Questions concerning the capability of the hybrids to become infected with <i>Trypanosoma cruzi</i> have been raised. This study aimed to compare the susceptibility of <i>Triatoma b. brasiliensis</i>, <i>Triatoma juazeirensis</i>, and their experimental hybrids to infection with <i>T. cruzi</i>. We infected the parentals and their experimental hybrids (obtained through reciprocal crosses) through artificial feeding with citrated rabbit blood, to which the TcI 0354 strain of <i>T. cruzi</i> had been added. The insects were weighed before and after feeding on the rabbit blood, and then they were dissected on the 10th, 20th, and 30th day after infection. Both the hybrids and the parentals remained infected throughout the experiment. The parasite was mostly found in the epimastigote form. The number of epimastigotes was significantly lower in the stomach and small intestine of <i>T. juazeirensis</i> than in the hybrids or in <i>T. b. brasiliensis</i>. A significantly higher percentage of metacyclic trypomastigotes was detected in the small intestine and rectum of the hybrids. Hybrids demonstrated higher susceptibility to the TcI 0354 strain than their parentals, opening up new avenues to be investigated. |
| first_indexed | 2024-03-08T20:32:26Z |
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| id | doaj.art-e8efbca1414a43afbbe6ecea45abecb2 |
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| language | English |
| last_indexed | 2024-03-08T20:32:26Z |
| publishDate | 2023-11-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-e8efbca1414a43afbbe6ecea45abecb22023-12-22T14:25:43ZengMDPI AGMicroorganisms2076-26072023-11-011112285010.3390/microorganisms11122850Experimental Hybrids of the <i>Triatoma brasiliensis</i> Species Complex Show Higher Susceptibility to the <i>Trypanosoma cruzi</i> Infection Than Their ParentalsNathália Correia0Letícia Paschoaletto1Carolina Reigada2Teresa Cristina Monte Gonçalves3Carlos José de Carvalho Moreira4Jane Costa5Entomology Laboratory, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-360, BrazilMolecular Parasitology Laboratory, Federal University of Rio de Janeiro, Rio de Janeiro 21941-901, BrazilCenter of Biological and Health Sciences, Department of Ecology and Evolutionary Biology, São Carlos University, UFSCAR, São Carlos 13565-905, BrazilInterdisciplinary Laboratory for Entomological Surveillance of Diptera and Hemiptera, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-360, BrazilParasitic Diseases Laboratory, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-360, BrazilNational and International Reference Laboratory in Taxonomy of Triatomines, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-360, BrazilThe <i>Triatoma brasiliensis</i> species complex is a monophyletic group encompassing two subspecies and six species. Recently, a hybrid zone of members of this complex was recorded in the state of Pernambuco. Questions concerning the capability of the hybrids to become infected with <i>Trypanosoma cruzi</i> have been raised. This study aimed to compare the susceptibility of <i>Triatoma b. brasiliensis</i>, <i>Triatoma juazeirensis</i>, and their experimental hybrids to infection with <i>T. cruzi</i>. We infected the parentals and their experimental hybrids (obtained through reciprocal crosses) through artificial feeding with citrated rabbit blood, to which the TcI 0354 strain of <i>T. cruzi</i> had been added. The insects were weighed before and after feeding on the rabbit blood, and then they were dissected on the 10th, 20th, and 30th day after infection. Both the hybrids and the parentals remained infected throughout the experiment. The parasite was mostly found in the epimastigote form. The number of epimastigotes was significantly lower in the stomach and small intestine of <i>T. juazeirensis</i> than in the hybrids or in <i>T. b. brasiliensis</i>. A significantly higher percentage of metacyclic trypomastigotes was detected in the small intestine and rectum of the hybrids. Hybrids demonstrated higher susceptibility to the TcI 0354 strain than their parentals, opening up new avenues to be investigated.https://www.mdpi.com/2076-2607/11/12/2850Chagas diseasetriatominehybridvector capacitymetacyclogenesisepimastigotes |
| spellingShingle | Nathália Correia Letícia Paschoaletto Carolina Reigada Teresa Cristina Monte Gonçalves Carlos José de Carvalho Moreira Jane Costa Experimental Hybrids of the <i>Triatoma brasiliensis</i> Species Complex Show Higher Susceptibility to the <i>Trypanosoma cruzi</i> Infection Than Their Parentals Microorganisms Chagas disease triatomine hybrid vector capacity metacyclogenesis epimastigotes |
| title | Experimental Hybrids of the <i>Triatoma brasiliensis</i> Species Complex Show Higher Susceptibility to the <i>Trypanosoma cruzi</i> Infection Than Their Parentals |
| title_full | Experimental Hybrids of the <i>Triatoma brasiliensis</i> Species Complex Show Higher Susceptibility to the <i>Trypanosoma cruzi</i> Infection Than Their Parentals |
| title_fullStr | Experimental Hybrids of the <i>Triatoma brasiliensis</i> Species Complex Show Higher Susceptibility to the <i>Trypanosoma cruzi</i> Infection Than Their Parentals |
| title_full_unstemmed | Experimental Hybrids of the <i>Triatoma brasiliensis</i> Species Complex Show Higher Susceptibility to the <i>Trypanosoma cruzi</i> Infection Than Their Parentals |
| title_short | Experimental Hybrids of the <i>Triatoma brasiliensis</i> Species Complex Show Higher Susceptibility to the <i>Trypanosoma cruzi</i> Infection Than Their Parentals |
| title_sort | experimental hybrids of the i triatoma brasiliensis i species complex show higher susceptibility to the i trypanosoma cruzi i infection than their parentals |
| topic | Chagas disease triatomine hybrid vector capacity metacyclogenesis epimastigotes |
| url | https://www.mdpi.com/2076-2607/11/12/2850 |
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