Leptospira interrogans enters different host cells by binding to distinct molecules in the extracellular matrix
Leptospirosis caused by Leptospira is a worldwide zoonotic disease. The 2 spirochete can efficiently invade host cells and spread from blood into internal organs, but the mechanisms of leptospiral internalization into different host cells remain poorly understood. Adhesion of leptospires to...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2015-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641400003Z.pdf |
| _version_ | 1817993152322076672 |
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| author | Zhao Xin Sun Ai-Hua Ge Yu-Mei Wang Huan Yan Jie |
| author_facet | Zhao Xin Sun Ai-Hua Ge Yu-Mei Wang Huan Yan Jie |
| author_sort | Zhao Xin |
| collection | DOAJ |
| description | Leptospirosis caused by Leptospira is a worldwide zoonotic disease. The 2
spirochete can efficiently invade host cells and spread from blood into
internal organs, but the mechanisms of leptospiral internalization into
different host cells remain poorly understood. Adhesion of leptospires to the
ECM (extracellular matrix) initiated leptospire internalization in macrophage
cell-lines THP-1, J774A.1, and in human umbilical epithelial cells (HUVEC).
We assessed the distribution of ECM , including fibronectin (FN), laminin and
collagens 1-4 (COL1-COL4) in different cells. FN was expressed in THP-1 and
J774A.1 cells, and FN, LN, COL3 and COL4 were expressed in HUVEC. Then, we
found leptospires adhered to FN, LN, COL3 and COL4. The most important
intracellular leptospires in macrophages (THP-1 and J774A) significantly
decreased when leptospires were pre-blocked with FN This indicates that FN
has an important role in the adherence to and entry of leptospires in
macrophages, while in HUVEC, FN, LN, COL3 and COL4 assume this role. |
| first_indexed | 2024-04-14T01:36:10Z |
| format | Article |
| id | doaj.art-e8f2c8dc0c744924b5f01ba17569792c |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-04-14T01:36:10Z |
| publishDate | 2015-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-e8f2c8dc0c744924b5f01ba17569792c2022-12-22T02:19:57ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392015-01-01671313910.2298/ABS140702003Z0354-46641400003ZLeptospira interrogans enters different host cells by binding to distinct molecules in the extracellular matrixZhao Xin0Sun Ai-Hua1Ge Yu-Mei2Wang Huan3Yan Jie4Zhejiang University School of Medicine, the First Affiliated Hospital, Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhejiang, P.R. China + Zhejiang University School of MedicineZhejiang Medical College, Faculty of Basic Medicine, Hangzhou, Zhejiang, P.R. ChinaZhejiang University School of Medicine, the First Affiliated Hospital, Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhejiang, P.R. China + Zhejiang University School of MedicineDepartment of Laboratory Medicine, the People’s Hospital of Zhejiang Province, Hangzhou, Zhejiang, P.R. ChinaZhejiang University School of Medicine, the First Affiliated Hospital, Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhejiang, P.R. China + Zhejiang University School of MedicineLeptospirosis caused by Leptospira is a worldwide zoonotic disease. The 2 spirochete can efficiently invade host cells and spread from blood into internal organs, but the mechanisms of leptospiral internalization into different host cells remain poorly understood. Adhesion of leptospires to the ECM (extracellular matrix) initiated leptospire internalization in macrophage cell-lines THP-1, J774A.1, and in human umbilical epithelial cells (HUVEC). We assessed the distribution of ECM , including fibronectin (FN), laminin and collagens 1-4 (COL1-COL4) in different cells. FN was expressed in THP-1 and J774A.1 cells, and FN, LN, COL3 and COL4 were expressed in HUVEC. Then, we found leptospires adhered to FN, LN, COL3 and COL4. The most important intracellular leptospires in macrophages (THP-1 and J774A) significantly decreased when leptospires were pre-blocked with FN This indicates that FN has an important role in the adherence to and entry of leptospires in macrophages, while in HUVEC, FN, LN, COL3 and COL4 assume this role.http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641400003Z.pdfLeptospira interrogansextracellular matrixadhesioninternalization |
| spellingShingle | Zhao Xin Sun Ai-Hua Ge Yu-Mei Wang Huan Yan Jie Leptospira interrogans enters different host cells by binding to distinct molecules in the extracellular matrix Archives of Biological Sciences Leptospira interrogans extracellular matrix adhesion internalization |
| title | Leptospira interrogans enters different host cells by binding to distinct molecules in the extracellular matrix |
| title_full | Leptospira interrogans enters different host cells by binding to distinct molecules in the extracellular matrix |
| title_fullStr | Leptospira interrogans enters different host cells by binding to distinct molecules in the extracellular matrix |
| title_full_unstemmed | Leptospira interrogans enters different host cells by binding to distinct molecules in the extracellular matrix |
| title_short | Leptospira interrogans enters different host cells by binding to distinct molecules in the extracellular matrix |
| title_sort | leptospira interrogans enters different host cells by binding to distinct molecules in the extracellular matrix |
| topic | Leptospira interrogans extracellular matrix adhesion internalization |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641400003Z.pdf |
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