Understanding how different surfaces and environmental biofilms found in food processing plants affect the spread of COVID-19.
Meat processing plants have been at the center of the SARS-CoV-2 pandemic, with a recent report citing 90% of US facilities having multiple outbreaks during 2020 and 2021. We explored the potential for biofilms to act as a reservoir in protecting, harboring, and dispersing SARS-CoV-2 throughout the...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0286659 |
| _version_ | 1797637490128977920 |
|---|---|
| author | Austin Featherstone Amanda Claire Brown Sapna Chitlapilly Dass |
| author_facet | Austin Featherstone Amanda Claire Brown Sapna Chitlapilly Dass |
| author_sort | Austin Featherstone |
| collection | DOAJ |
| description | Meat processing plants have been at the center of the SARS-CoV-2 pandemic, with a recent report citing 90% of US facilities having multiple outbreaks during 2020 and 2021. We explored the potential for biofilms to act as a reservoir in protecting, harboring, and dispersing SARS-CoV-2 throughout the meat processing facility environment. To do this, we used Murine Hepatitis Virus (MHV), as a surrogate for SARS-CoV-2, and meat processing facility drain samples to develop mixed-species biofilms on materials found in meat processing facilities (stainless steel (SS), PVC, and ceramic tiles). After exposure to the biofilm organisms for five days post-inoculation at 7°C we conducted quantitative PCR (qPCR) and plaque assays to determine whether MHV could remain both detectable and viable. Our data provides evidence that coronaviruses can remain viable on all the surfaces tested and are also able to integrate within an environmental biofilm. Although a portion of MHV was able to remain infectious after incubation with the environmental biofilm, a large reduction in plaque numbers was identified when compared with the viral inoculum incubated without biofilm on all test surfaces, which ranged from 6.45-9.27-fold higher. Interestingly, we observed a 2-fold increase in the virus-environmental biofilm biovolume when compared to biofilm without virus, indicating that the biofilm bacteria both detected and reacted to the virus. These results indicate a complex virus-environmental biofilm interaction. Although we observed better survival of MHV on a variety of surfaces commonly found in meat processing plants alone than with the biofilm, there is the potential for biofilms to protect virions from disinfecting agents, which has implications for the potential of SARS-CoV-2 prevalence within the meat processing plant environment. Also given the highly infectious nature of SARS-CoV-2, particularly for some of the variant strains such as omicron, having even a residual level of virus present represents a serious health hazard. The increase in biofilm biovolume in response to virus is also a concern for food safety due to the potential of the same being seen with organisms associated with food poisoning and food spoilage. |
| first_indexed | 2024-03-11T12:50:10Z |
| format | Article |
| id | doaj.art-e8f9b6afa0184b748def991f2a77b57f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-11T12:50:10Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e8f9b6afa0184b748def991f2a77b57f2023-11-04T05:33:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01186e028665910.1371/journal.pone.0286659Understanding how different surfaces and environmental biofilms found in food processing plants affect the spread of COVID-19.Austin FeatherstoneAmanda Claire BrownSapna Chitlapilly DassMeat processing plants have been at the center of the SARS-CoV-2 pandemic, with a recent report citing 90% of US facilities having multiple outbreaks during 2020 and 2021. We explored the potential for biofilms to act as a reservoir in protecting, harboring, and dispersing SARS-CoV-2 throughout the meat processing facility environment. To do this, we used Murine Hepatitis Virus (MHV), as a surrogate for SARS-CoV-2, and meat processing facility drain samples to develop mixed-species biofilms on materials found in meat processing facilities (stainless steel (SS), PVC, and ceramic tiles). After exposure to the biofilm organisms for five days post-inoculation at 7°C we conducted quantitative PCR (qPCR) and plaque assays to determine whether MHV could remain both detectable and viable. Our data provides evidence that coronaviruses can remain viable on all the surfaces tested and are also able to integrate within an environmental biofilm. Although a portion of MHV was able to remain infectious after incubation with the environmental biofilm, a large reduction in plaque numbers was identified when compared with the viral inoculum incubated without biofilm on all test surfaces, which ranged from 6.45-9.27-fold higher. Interestingly, we observed a 2-fold increase in the virus-environmental biofilm biovolume when compared to biofilm without virus, indicating that the biofilm bacteria both detected and reacted to the virus. These results indicate a complex virus-environmental biofilm interaction. Although we observed better survival of MHV on a variety of surfaces commonly found in meat processing plants alone than with the biofilm, there is the potential for biofilms to protect virions from disinfecting agents, which has implications for the potential of SARS-CoV-2 prevalence within the meat processing plant environment. Also given the highly infectious nature of SARS-CoV-2, particularly for some of the variant strains such as omicron, having even a residual level of virus present represents a serious health hazard. The increase in biofilm biovolume in response to virus is also a concern for food safety due to the potential of the same being seen with organisms associated with food poisoning and food spoilage.https://doi.org/10.1371/journal.pone.0286659 |
| spellingShingle | Austin Featherstone Amanda Claire Brown Sapna Chitlapilly Dass Understanding how different surfaces and environmental biofilms found in food processing plants affect the spread of COVID-19. PLoS ONE |
| title | Understanding how different surfaces and environmental biofilms found in food processing plants affect the spread of COVID-19. |
| title_full | Understanding how different surfaces and environmental biofilms found in food processing plants affect the spread of COVID-19. |
| title_fullStr | Understanding how different surfaces and environmental biofilms found in food processing plants affect the spread of COVID-19. |
| title_full_unstemmed | Understanding how different surfaces and environmental biofilms found in food processing plants affect the spread of COVID-19. |
| title_short | Understanding how different surfaces and environmental biofilms found in food processing plants affect the spread of COVID-19. |
| title_sort | understanding how different surfaces and environmental biofilms found in food processing plants affect the spread of covid 19 |
| url | https://doi.org/10.1371/journal.pone.0286659 |
| work_keys_str_mv | AT austinfeatherstone understandinghowdifferentsurfacesandenvironmentalbiofilmsfoundinfoodprocessingplantsaffectthespreadofcovid19 AT amandaclairebrown understandinghowdifferentsurfacesandenvironmentalbiofilmsfoundinfoodprocessingplantsaffectthespreadofcovid19 AT sapnachitlapillydass understandinghowdifferentsurfacesandenvironmentalbiofilmsfoundinfoodprocessingplantsaffectthespreadofcovid19 |