pH and Nitrate Drive Bacterial Diversity in Oil Reservoirs at a Localized Geographic Scale
Oil reservoirs are one of the most important deep subsurface biospheres. They are inhabited by diverse microorganisms including bacteria and archaea with diverse metabolic activities. Although recent studies have investigated the microbial communities in oil reservoirs at large geographic scales, it...
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MDPI AG
2023-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/1/151 |
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| author | Ying Xu Jianwei Wang Qingjie Liu Qun Zhang Jiazhong Wu Minghui Zhou Yong Nie Xiao-Lei Wu |
| author_facet | Ying Xu Jianwei Wang Qingjie Liu Qun Zhang Jiazhong Wu Minghui Zhou Yong Nie Xiao-Lei Wu |
| author_sort | Ying Xu |
| collection | DOAJ |
| description | Oil reservoirs are one of the most important deep subsurface biospheres. They are inhabited by diverse microorganisms including bacteria and archaea with diverse metabolic activities. Although recent studies have investigated the microbial communities in oil reservoirs at large geographic scales, it is still not clear how the microbial communities assemble, as the variation in the environment may be confounded with geographic distance. In this work, the microbial communities in oil reservoirs from the same oil field were identified at a localized geographic scale. We found that although the injected water contained diverse exogenous microorganisms, this had little effect on the microbial composition of the produced water. The Neutral Community Model analysis showed that both bacterial and archaeal communities are dispersal limited even at a localized scale. Further analysis showed that both pH and nitrate concentrations drive the assembly of bacterial communities, of which nitrate negatively correlated with bacterial alpha diversity and pH differences positively correlated with the dissimilarity of bacterial communities. In contrast, the physiochemical parameters had little effect on archaeal communities at the localized scale. Our results suggest that the assembly of microbial communities in oil reservoirs is scale- and taxonomy-dependent. Our work provides a comprehensive analysis of microbial communities in oil reservoirs at a localized geographic scale, which improves the understanding of the assembly of the microbial communities in oil reservoirs. |
| first_indexed | 2024-03-09T11:38:41Z |
| format | Article |
| id | doaj.art-e8fcee44652041adadad28652946974b |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T11:38:41Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-e8fcee44652041adadad28652946974b2023-11-30T23:37:11ZengMDPI AGMicroorganisms2076-26072023-01-0111115110.3390/microorganisms11010151pH and Nitrate Drive Bacterial Diversity in Oil Reservoirs at a Localized Geographic ScaleYing Xu0Jianwei Wang1Qingjie Liu2Qun Zhang3Jiazhong Wu4Minghui Zhou5Yong Nie6Xiao-Lei Wu7State Key Laboratory of Enhanced Oil Recovery, PetroChina Research Institute of Petroleum Exploration & Development, Beijing 100083, ChinaCollege of Engineering, Peking University, Beijing 100871, ChinaState Key Laboratory of Enhanced Oil Recovery, PetroChina Research Institute of Petroleum Exploration & Development, Beijing 100083, ChinaState Key Laboratory of Enhanced Oil Recovery, PetroChina Research Institute of Petroleum Exploration & Development, Beijing 100083, ChinaState Key Laboratory of Enhanced Oil Recovery, PetroChina Research Institute of Petroleum Exploration & Development, Beijing 100083, ChinaState Key Laboratory of Enhanced Oil Recovery, PetroChina Research Institute of Petroleum Exploration & Development, Beijing 100083, ChinaCollege of Engineering, Peking University, Beijing 100871, ChinaCollege of Engineering, Peking University, Beijing 100871, ChinaOil reservoirs are one of the most important deep subsurface biospheres. They are inhabited by diverse microorganisms including bacteria and archaea with diverse metabolic activities. Although recent studies have investigated the microbial communities in oil reservoirs at large geographic scales, it is still not clear how the microbial communities assemble, as the variation in the environment may be confounded with geographic distance. In this work, the microbial communities in oil reservoirs from the same oil field were identified at a localized geographic scale. We found that although the injected water contained diverse exogenous microorganisms, this had little effect on the microbial composition of the produced water. The Neutral Community Model analysis showed that both bacterial and archaeal communities are dispersal limited even at a localized scale. Further analysis showed that both pH and nitrate concentrations drive the assembly of bacterial communities, of which nitrate negatively correlated with bacterial alpha diversity and pH differences positively correlated with the dissimilarity of bacterial communities. In contrast, the physiochemical parameters had little effect on archaeal communities at the localized scale. Our results suggest that the assembly of microbial communities in oil reservoirs is scale- and taxonomy-dependent. Our work provides a comprehensive analysis of microbial communities in oil reservoirs at a localized geographic scale, which improves the understanding of the assembly of the microbial communities in oil reservoirs.https://www.mdpi.com/2076-2607/11/1/151microbial communityoil reservoirassemblymicrobial diversity |
| spellingShingle | Ying Xu Jianwei Wang Qingjie Liu Qun Zhang Jiazhong Wu Minghui Zhou Yong Nie Xiao-Lei Wu pH and Nitrate Drive Bacterial Diversity in Oil Reservoirs at a Localized Geographic Scale Microorganisms microbial community oil reservoir assembly microbial diversity |
| title | pH and Nitrate Drive Bacterial Diversity in Oil Reservoirs at a Localized Geographic Scale |
| title_full | pH and Nitrate Drive Bacterial Diversity in Oil Reservoirs at a Localized Geographic Scale |
| title_fullStr | pH and Nitrate Drive Bacterial Diversity in Oil Reservoirs at a Localized Geographic Scale |
| title_full_unstemmed | pH and Nitrate Drive Bacterial Diversity in Oil Reservoirs at a Localized Geographic Scale |
| title_short | pH and Nitrate Drive Bacterial Diversity in Oil Reservoirs at a Localized Geographic Scale |
| title_sort | ph and nitrate drive bacterial diversity in oil reservoirs at a localized geographic scale |
| topic | microbial community oil reservoir assembly microbial diversity |
| url | https://www.mdpi.com/2076-2607/11/1/151 |
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