Sour ageusia in two individuals implicates ion channels of the ASIC and PKD families in human sour taste perception at the anterior tongue.
BACKGROUND:The perception of sour taste in humans is incompletely understood at the receptor cell level. We report here on two patients with an acquired sour ageusia. Each patient was unresponsive to sour stimuli, but both showed normal responses to bitter, sweet, and salty stimuli. METHODS AND FIND...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-10-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2754526?pdf=render |
| _version_ | 1819231686911590400 |
|---|---|
| author | Taufiqul Huque Beverly J Cowart Luba Dankulich-Nagrudny Edmund A Pribitkin Douglas L Bayley Andrew I Spielman Roy S Feldman Scott A Mackler Joseph G Brand |
| author_facet | Taufiqul Huque Beverly J Cowart Luba Dankulich-Nagrudny Edmund A Pribitkin Douglas L Bayley Andrew I Spielman Roy S Feldman Scott A Mackler Joseph G Brand |
| author_sort | Taufiqul Huque |
| collection | DOAJ |
| description | BACKGROUND:The perception of sour taste in humans is incompletely understood at the receptor cell level. We report here on two patients with an acquired sour ageusia. Each patient was unresponsive to sour stimuli, but both showed normal responses to bitter, sweet, and salty stimuli. METHODS AND FINDINGS:Lingual fungiform papillae, containing taste cells, were obtained by biopsy from the two patients, and from three sour-normal individuals, and analyzed by RT-PCR. The following transcripts were undetectable in the patients, even after 50 cycles of amplification, but readily detectable in the sour-normal subjects: acid sensing ion channels (ASICs) 1a, 1beta, 2a, 2b, and 3; and polycystic kidney disease (PKD) channels PKD1L3 and PKD2L1. Patients and sour-normals expressed the taste-related phospholipase C-beta2, the delta-subunit of epithelial sodium channel (ENaC) and the bitter receptor T2R14, as well as beta-actin. Genomic analysis of one patient, using buccal tissue, did not show absence of the genes for ASIC1a and PKD2L1. Immunohistochemistry of fungiform papillae from sour-normal subjects revealed labeling of taste bud cells by antibodies to ASICs 1a and 1beta, PKD2L1, phospholipase C-beta2, and delta-ENaC. An antibody to PKD1L3 labeled tissue outside taste bud cells. CONCLUSIONS:These data suggest a role for ASICs and PKDs in human sour perception. This is the first report of sour ageusia in humans, and the very existence of such individuals ("natural knockouts") suggests a cell lineage for sour that is independent of the other taste modalities. |
| first_indexed | 2024-12-23T11:48:55Z |
| format | Article |
| id | doaj.art-e8fda366c07147eaaac4634d19c495b3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T11:48:55Z |
| publishDate | 2009-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e8fda366c07147eaaac4634d19c495b32022-12-21T17:48:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-10-01410e734710.1371/journal.pone.0007347Sour ageusia in two individuals implicates ion channels of the ASIC and PKD families in human sour taste perception at the anterior tongue.Taufiqul HuqueBeverly J CowartLuba Dankulich-NagrudnyEdmund A PribitkinDouglas L BayleyAndrew I SpielmanRoy S FeldmanScott A MacklerJoseph G BrandBACKGROUND:The perception of sour taste in humans is incompletely understood at the receptor cell level. We report here on two patients with an acquired sour ageusia. Each patient was unresponsive to sour stimuli, but both showed normal responses to bitter, sweet, and salty stimuli. METHODS AND FINDINGS:Lingual fungiform papillae, containing taste cells, were obtained by biopsy from the two patients, and from three sour-normal individuals, and analyzed by RT-PCR. The following transcripts were undetectable in the patients, even after 50 cycles of amplification, but readily detectable in the sour-normal subjects: acid sensing ion channels (ASICs) 1a, 1beta, 2a, 2b, and 3; and polycystic kidney disease (PKD) channels PKD1L3 and PKD2L1. Patients and sour-normals expressed the taste-related phospholipase C-beta2, the delta-subunit of epithelial sodium channel (ENaC) and the bitter receptor T2R14, as well as beta-actin. Genomic analysis of one patient, using buccal tissue, did not show absence of the genes for ASIC1a and PKD2L1. Immunohistochemistry of fungiform papillae from sour-normal subjects revealed labeling of taste bud cells by antibodies to ASICs 1a and 1beta, PKD2L1, phospholipase C-beta2, and delta-ENaC. An antibody to PKD1L3 labeled tissue outside taste bud cells. CONCLUSIONS:These data suggest a role for ASICs and PKDs in human sour perception. This is the first report of sour ageusia in humans, and the very existence of such individuals ("natural knockouts") suggests a cell lineage for sour that is independent of the other taste modalities.http://europepmc.org/articles/PMC2754526?pdf=render |
| spellingShingle | Taufiqul Huque Beverly J Cowart Luba Dankulich-Nagrudny Edmund A Pribitkin Douglas L Bayley Andrew I Spielman Roy S Feldman Scott A Mackler Joseph G Brand Sour ageusia in two individuals implicates ion channels of the ASIC and PKD families in human sour taste perception at the anterior tongue. PLoS ONE |
| title | Sour ageusia in two individuals implicates ion channels of the ASIC and PKD families in human sour taste perception at the anterior tongue. |
| title_full | Sour ageusia in two individuals implicates ion channels of the ASIC and PKD families in human sour taste perception at the anterior tongue. |
| title_fullStr | Sour ageusia in two individuals implicates ion channels of the ASIC and PKD families in human sour taste perception at the anterior tongue. |
| title_full_unstemmed | Sour ageusia in two individuals implicates ion channels of the ASIC and PKD families in human sour taste perception at the anterior tongue. |
| title_short | Sour ageusia in two individuals implicates ion channels of the ASIC and PKD families in human sour taste perception at the anterior tongue. |
| title_sort | sour ageusia in two individuals implicates ion channels of the asic and pkd families in human sour taste perception at the anterior tongue |
| url | http://europepmc.org/articles/PMC2754526?pdf=render |
| work_keys_str_mv | AT taufiqulhuque sourageusiaintwoindividualsimplicatesionchannelsoftheasicandpkdfamiliesinhumansourtasteperceptionattheanteriortongue AT beverlyjcowart sourageusiaintwoindividualsimplicatesionchannelsoftheasicandpkdfamiliesinhumansourtasteperceptionattheanteriortongue AT lubadankulichnagrudny sourageusiaintwoindividualsimplicatesionchannelsoftheasicandpkdfamiliesinhumansourtasteperceptionattheanteriortongue AT edmundapribitkin sourageusiaintwoindividualsimplicatesionchannelsoftheasicandpkdfamiliesinhumansourtasteperceptionattheanteriortongue AT douglaslbayley sourageusiaintwoindividualsimplicatesionchannelsoftheasicandpkdfamiliesinhumansourtasteperceptionattheanteriortongue AT andrewispielman sourageusiaintwoindividualsimplicatesionchannelsoftheasicandpkdfamiliesinhumansourtasteperceptionattheanteriortongue AT roysfeldman sourageusiaintwoindividualsimplicatesionchannelsoftheasicandpkdfamiliesinhumansourtasteperceptionattheanteriortongue AT scottamackler sourageusiaintwoindividualsimplicatesionchannelsoftheasicandpkdfamiliesinhumansourtasteperceptionattheanteriortongue AT josephgbrand sourageusiaintwoindividualsimplicatesionchannelsoftheasicandpkdfamiliesinhumansourtasteperceptionattheanteriortongue |