FTIR Spectral and Microarchitectural Analysis of Cellulose Produced by Lactococcus lactis Under Agitated Condition

The demand for cellulose is accelerating in the paper making industry. Alternate sources of cellulose has to be traced in order to reduce the demand for plant cellulose. Hence, in this study bacterial cellulose has been chosen as an option. In this study, the potential of soil bacteria Lactococcus l...

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Main Authors: S. Umamaheswari, S. Malkar Oli, K. Naveena
Format: Article
Language:English
Published: Journal of Pure and Applied Microbiology 2017-12-01
Series:Journal of Pure and Applied Microbiology
Subjects:
Online Access:https://microbiologyjournal.org/ftir-spectral-and-microarchitecturalanalysis-of-cellulose-produced-by-lactococcuslactis-under-agitated-condition/
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author S. Umamaheswari
S. Malkar Oli
K. Naveena
author_facet S. Umamaheswari
S. Malkar Oli
K. Naveena
author_sort S. Umamaheswari
collection DOAJ
description The demand for cellulose is accelerating in the paper making industry. Alternate sources of cellulose has to be traced in order to reduce the demand for plant cellulose. Hence, in this study bacterial cellulose has been chosen as an option. In this study, the potential of soil bacteria Lactococcus lactis to produce cellulose has been assessed. The results obtained indicate that the inocula size of the bacteria had a vital role in altering the quantity of cellulose produced. Among the inocula size, 100 µl of broth culture exhibited highest production of cellulose. The cellulose produced was characterised spectrally and its microarchitectural study reveal its crystalline nature. FTIR spectra of the bacterial cellulose produced depict the signature peak of bacterial cellulose. Based on these observations, it could be concluded that Lactococcus lactis produce cellulose. Further studies has to be carried out to optimize the bacterial cellulose production.
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spelling doaj.art-e8ffcea10a424dc4ba5affa2868d66ad2022-12-21T17:16:05ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2017-12-011141965197110.22207/JPAM.11.4.38FTIR Spectral and Microarchitectural Analysis of Cellulose Produced by Lactococcus lactis Under Agitated ConditionS. Umamaheswari0S. Malkar Oli 1K. Naveena2P.G and Research Department of Zoology, Periyar EVR College, Tiruchirappalli - 620 023, Tamil Nadu, India.P.G and Research Department of Zoology, Periyar EVR College, Tiruchirappalli - 620 023, Tamil Nadu, India.P.G and Research Department of Zoology, Periyar EVR College, Tiruchirappalli - 620 023, Tamil Nadu, India.The demand for cellulose is accelerating in the paper making industry. Alternate sources of cellulose has to be traced in order to reduce the demand for plant cellulose. Hence, in this study bacterial cellulose has been chosen as an option. In this study, the potential of soil bacteria Lactococcus lactis to produce cellulose has been assessed. The results obtained indicate that the inocula size of the bacteria had a vital role in altering the quantity of cellulose produced. Among the inocula size, 100 µl of broth culture exhibited highest production of cellulose. The cellulose produced was characterised spectrally and its microarchitectural study reveal its crystalline nature. FTIR spectra of the bacterial cellulose produced depict the signature peak of bacterial cellulose. Based on these observations, it could be concluded that Lactococcus lactis produce cellulose. Further studies has to be carried out to optimize the bacterial cellulose production.https://microbiologyjournal.org/ftir-spectral-and-microarchitecturalanalysis-of-cellulose-produced-by-lactococcuslactis-under-agitated-condition/lactococcus lactiscelluloseglucoseftirsem.
spellingShingle S. Umamaheswari
S. Malkar Oli
K. Naveena
FTIR Spectral and Microarchitectural Analysis of Cellulose Produced by Lactococcus lactis Under Agitated Condition
Journal of Pure and Applied Microbiology
lactococcus lactis
cellulose
glucose
ftir
sem.
title FTIR Spectral and Microarchitectural Analysis of Cellulose Produced by Lactococcus lactis Under Agitated Condition
title_full FTIR Spectral and Microarchitectural Analysis of Cellulose Produced by Lactococcus lactis Under Agitated Condition
title_fullStr FTIR Spectral and Microarchitectural Analysis of Cellulose Produced by Lactococcus lactis Under Agitated Condition
title_full_unstemmed FTIR Spectral and Microarchitectural Analysis of Cellulose Produced by Lactococcus lactis Under Agitated Condition
title_short FTIR Spectral and Microarchitectural Analysis of Cellulose Produced by Lactococcus lactis Under Agitated Condition
title_sort ftir spectral and microarchitectural analysis of cellulose produced by lactococcus lactis under agitated condition
topic lactococcus lactis
cellulose
glucose
ftir
sem.
url https://microbiologyjournal.org/ftir-spectral-and-microarchitecturalanalysis-of-cellulose-produced-by-lactococcuslactis-under-agitated-condition/
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AT knaveena ftirspectralandmicroarchitecturalanalysisofcelluloseproducedbylactococcuslactisunderagitatedcondition