Olive agroforestry shapes rhizosphere microbiome networks associated with annual crops and impacts the biomass production under low-rainfed conditions
Agroforestry (AF) is a promising land-use system to mitigate water deficiency, particularly in semi-arid areas. However, the belowground microbes associated with crops below trees remain seldom addressed. This study aimed at elucidating the effects of olive AF system intercropped with durum wheat (D...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-10-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.977797/full |
| _version_ | 1811197290895900672 |
|---|---|
| author | Ameni Ben zineb Karim Barkaoui Karim Barkaoui Fatma Karray Najla Mhiri Sami Sayadi Ahmed Mliki Mahmoud Gargouri |
| author_facet | Ameni Ben zineb Karim Barkaoui Karim Barkaoui Fatma Karray Najla Mhiri Sami Sayadi Ahmed Mliki Mahmoud Gargouri |
| author_sort | Ameni Ben zineb |
| collection | DOAJ |
| description | Agroforestry (AF) is a promising land-use system to mitigate water deficiency, particularly in semi-arid areas. However, the belowground microbes associated with crops below trees remain seldom addressed. This study aimed at elucidating the effects of olive AF system intercropped with durum wheat (Dw), barely (Ba), chickpea (Cp), or faba bean (Fb) on crops biomass and their soil-rhizosphere microbial networks as compared to conventional full sun cropping (SC) under rainfed conditions. To test the hypothesis, we compared the prokaryotic and the fungal communities inhabiting the rhizosphere of two cereals and legumes grown either in AF or SC. We determined the most suitable annual crop species in AF under low-rainfed conditions. Moreover, to deepen our understanding of the rhizosphere network dynamics of annual crops under AF and SC systems, we characterized the microbial hubs that are most likely responsible for modifying the microbial community structure and the variability of crop biomass of each species. Herein, we found that cereals produced significantly more above-ground biomass than legumes following in descending order: Ba > Dw > Cp > Fb, suggesting that crop species play a significant role in improving soil water use and that cereals are well-suited to rainfed conditions within both types of agrosystems. The type of agrosystem shapes crop microbiomes with the only marginal influence of host selection. However, more relevant was to unveil those crops recruits specific bacterial and fungal taxa from the olive-belowground communities. Of the selected soil physicochemical properties, organic matter was the principal driver in shaping the soil microbial structure in the AF system. The co-occurrence network analyses indicated that the AF system generates higher ecological stability than the SC system under stressful climate conditions. Furthermore, legumes’ rhizosphere microbiome possessed a higher resilient capacity than cereals. We also identified different fungal keystones involved in litter decomposition and drought tolerance within AF systems facing the water-scarce condition and promoting crop production within the SC system. Overall, we showed that AF reduces cereal and legume rhizosphere microbial diversity, enhances network complexity, and leads to more stable beneficial microbial communities, especially in severe drought, thus providing more accurate predictions to preserve soil diversity under unfavorable environmental conditions. |
| first_indexed | 2024-04-12T01:11:36Z |
| format | Article |
| id | doaj.art-e901dad2e654448b83ac09924dac25da |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T01:11:36Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-e901dad2e654448b83ac09924dac25da2022-12-22T03:54:05ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-10-011310.3389/fmicb.2022.977797977797Olive agroforestry shapes rhizosphere microbiome networks associated with annual crops and impacts the biomass production under low-rainfed conditionsAmeni Ben zineb0Karim Barkaoui1Karim Barkaoui2Fatma Karray3Najla Mhiri4Sami Sayadi5Ahmed Mliki6Mahmoud Gargouri7Laboratory of Plant Molecular Physiology, Centre of Biotechnology of Borj-Cedria, Hammam-Lif, TunisiaCIRAD, UMR ABSys, Montpellier, FranceABSys, Univ Montpellier, CIHEAM-IAMM, CIRAD, INRAE, Institut Agro, Montpellier, FranceLaboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, Sfax, TunisiaLaboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, Sfax, TunisiaBiotechnology Program, Center for Sustainable Development, College of Arts and Sciences, Qatar University, Doha, QatarLaboratory of Plant Molecular Physiology, Centre of Biotechnology of Borj-Cedria, Hammam-Lif, TunisiaLaboratory of Plant Molecular Physiology, Centre of Biotechnology of Borj-Cedria, Hammam-Lif, TunisiaAgroforestry (AF) is a promising land-use system to mitigate water deficiency, particularly in semi-arid areas. However, the belowground microbes associated with crops below trees remain seldom addressed. This study aimed at elucidating the effects of olive AF system intercropped with durum wheat (Dw), barely (Ba), chickpea (Cp), or faba bean (Fb) on crops biomass and their soil-rhizosphere microbial networks as compared to conventional full sun cropping (SC) under rainfed conditions. To test the hypothesis, we compared the prokaryotic and the fungal communities inhabiting the rhizosphere of two cereals and legumes grown either in AF or SC. We determined the most suitable annual crop species in AF under low-rainfed conditions. Moreover, to deepen our understanding of the rhizosphere network dynamics of annual crops under AF and SC systems, we characterized the microbial hubs that are most likely responsible for modifying the microbial community structure and the variability of crop biomass of each species. Herein, we found that cereals produced significantly more above-ground biomass than legumes following in descending order: Ba > Dw > Cp > Fb, suggesting that crop species play a significant role in improving soil water use and that cereals are well-suited to rainfed conditions within both types of agrosystems. The type of agrosystem shapes crop microbiomes with the only marginal influence of host selection. However, more relevant was to unveil those crops recruits specific bacterial and fungal taxa from the olive-belowground communities. Of the selected soil physicochemical properties, organic matter was the principal driver in shaping the soil microbial structure in the AF system. The co-occurrence network analyses indicated that the AF system generates higher ecological stability than the SC system under stressful climate conditions. Furthermore, legumes’ rhizosphere microbiome possessed a higher resilient capacity than cereals. We also identified different fungal keystones involved in litter decomposition and drought tolerance within AF systems facing the water-scarce condition and promoting crop production within the SC system. Overall, we showed that AF reduces cereal and legume rhizosphere microbial diversity, enhances network complexity, and leads to more stable beneficial microbial communities, especially in severe drought, thus providing more accurate predictions to preserve soil diversity under unfavorable environmental conditions.https://www.frontiersin.org/articles/10.3389/fmicb.2022.977797/fullolive agroforestrysun cropping systemsoil-rhizospheremicrobial network keystonescerealslegumes |
| spellingShingle | Ameni Ben zineb Karim Barkaoui Karim Barkaoui Fatma Karray Najla Mhiri Sami Sayadi Ahmed Mliki Mahmoud Gargouri Olive agroforestry shapes rhizosphere microbiome networks associated with annual crops and impacts the biomass production under low-rainfed conditions Frontiers in Microbiology olive agroforestry sun cropping system soil-rhizosphere microbial network keystones cereals legumes |
| title | Olive agroforestry shapes rhizosphere microbiome networks associated with annual crops and impacts the biomass production under low-rainfed conditions |
| title_full | Olive agroforestry shapes rhizosphere microbiome networks associated with annual crops and impacts the biomass production under low-rainfed conditions |
| title_fullStr | Olive agroforestry shapes rhizosphere microbiome networks associated with annual crops and impacts the biomass production under low-rainfed conditions |
| title_full_unstemmed | Olive agroforestry shapes rhizosphere microbiome networks associated with annual crops and impacts the biomass production under low-rainfed conditions |
| title_short | Olive agroforestry shapes rhizosphere microbiome networks associated with annual crops and impacts the biomass production under low-rainfed conditions |
| title_sort | olive agroforestry shapes rhizosphere microbiome networks associated with annual crops and impacts the biomass production under low rainfed conditions |
| topic | olive agroforestry sun cropping system soil-rhizosphere microbial network keystones cereals legumes |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.977797/full |
| work_keys_str_mv | AT amenibenzineb oliveagroforestryshapesrhizospheremicrobiomenetworksassociatedwithannualcropsandimpactsthebiomassproductionunderlowrainfedconditions AT karimbarkaoui oliveagroforestryshapesrhizospheremicrobiomenetworksassociatedwithannualcropsandimpactsthebiomassproductionunderlowrainfedconditions AT karimbarkaoui oliveagroforestryshapesrhizospheremicrobiomenetworksassociatedwithannualcropsandimpactsthebiomassproductionunderlowrainfedconditions AT fatmakarray oliveagroforestryshapesrhizospheremicrobiomenetworksassociatedwithannualcropsandimpactsthebiomassproductionunderlowrainfedconditions AT najlamhiri oliveagroforestryshapesrhizospheremicrobiomenetworksassociatedwithannualcropsandimpactsthebiomassproductionunderlowrainfedconditions AT samisayadi oliveagroforestryshapesrhizospheremicrobiomenetworksassociatedwithannualcropsandimpactsthebiomassproductionunderlowrainfedconditions AT ahmedmliki oliveagroforestryshapesrhizospheremicrobiomenetworksassociatedwithannualcropsandimpactsthebiomassproductionunderlowrainfedconditions AT mahmoudgargouri oliveagroforestryshapesrhizospheremicrobiomenetworksassociatedwithannualcropsandimpactsthebiomassproductionunderlowrainfedconditions |