Effect of conjugated linoleic acid and different type of dietary fat on serum lipid profile, liver enzymes activity and oxidative stress markers in Wistar rats
Background. Nutritional recommendations emphasize the need to limit consumption of saturated fatty acids and to increase the intake of polyunsaturated fatty acids in the prevention of non-communicable chronic diseases, particularly cardiovascular diseases. Among the fatty acids with health-related e...
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| Format: | Article |
| Language: | English |
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National Institute of Public Health - National Institute of Hygiene
2019-03-01
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| Series: | Roczniki Panstwowego Zakladu Higieny |
| Subjects: | |
| Online Access: | http://wydawnictwa.pzh.gov.pl/roczniki_pzh/pobierz-artykul?id=1268 |
| _version_ | 1819170221253984256 |
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| author | Magdalena Franczyk-Żarów Edyta Kuś Renata B. Kostogrys |
| author_facet | Magdalena Franczyk-Żarów Edyta Kuś Renata B. Kostogrys |
| author_sort | Magdalena Franczyk-Żarów |
| collection | DOAJ |
| description | Background. Nutritional recommendations emphasize the need to limit consumption of saturated fatty acids and to increase the intake of polyunsaturated fatty acids in the prevention of non-communicable chronic diseases, particularly cardiovascular diseases. Among the fatty acids with health-related effects on the body, conjugated fatty acids are mentioned (i.e. CLA).
Objective. The current study was designed to determine the effects of conjugated linoleic acid (CLA) on serum lipid profile, glucose, liver enzymes activity (AST and ALT), malonic dialdehyde (MDA) as well as lipid hydroperoxide (LPO) concentrations in rats fed diet differing in type of dietary fat.
Material and methods. Male Wistar rats were divided into six groups and fed the following diets: control AIN-93G diet contained soybean oil (O) and diets with modification of fat source: butter (B) and margarine (M). The experimental diets were supplemented with 1% of conjugated linoleic acid (O+CLA, B+CLA, M+CLA). After 21 days the blood was collected and lipid profile, glucose, liver enzymes, MDA as well as LPO were analyzed.
Results. The dietary treatments had no significant effect on the body weight and liver weight of the animals. The concentrations of total cholesterol (TC) and LDL+VLDL cholesterol were unchanged. Both experimental factors (fat source and CLA) had a significant influence on the TAG and HDL levels. Margarine (M) significantly increased the TAG concentration, whereas CLA had a significant impact on the TAG reduction (M+CLA). Glucose level was significantly decreased in all groups fed diets supplemented with CLA. Serum ALT significantly increased in all CLA groups. Fat source had statistically significant influence on the MDA concentration. The LPO level was significantly elevated in all CLA groups. There was statistically significant interaction of experimental factors (fat source and CLA supplementation) on LPO level.
Conclusions. Margarine had an adverse effect on the rat’s lipid profile. However, in the group fed with margarine, the addition of CLA decreased the concentration of TAG. Regardless of the type of the dietary fat, CLA supplementation increased the level of LPO in the blood serum of animals. |
| first_indexed | 2024-12-22T19:31:57Z |
| format | Article |
| id | doaj.art-e903d4bca9d8473dad8a81acd094a3a3 |
| institution | Directory Open Access Journal |
| issn | 0035-7715 2451-2311 |
| language | English |
| last_indexed | 2024-12-22T19:31:57Z |
| publishDate | 2019-03-01 |
| publisher | National Institute of Public Health - National Institute of Hygiene |
| record_format | Article |
| series | Roczniki Panstwowego Zakladu Higieny |
| spelling | doaj.art-e903d4bca9d8473dad8a81acd094a3a32022-12-21T18:15:05ZengNational Institute of Public Health - National Institute of HygieneRoczniki Panstwowego Zakladu Higieny0035-77152451-23112019-03-01701273310.32394/rpzh.2019.0049Effect of conjugated linoleic acid and different type of dietary fat on serum lipid profile, liver enzymes activity and oxidative stress markers in Wistar ratsMagdalena Franczyk-ŻarówEdyta KuśRenata B. KostogrysBackground. Nutritional recommendations emphasize the need to limit consumption of saturated fatty acids and to increase the intake of polyunsaturated fatty acids in the prevention of non-communicable chronic diseases, particularly cardiovascular diseases. Among the fatty acids with health-related effects on the body, conjugated fatty acids are mentioned (i.e. CLA). Objective. The current study was designed to determine the effects of conjugated linoleic acid (CLA) on serum lipid profile, glucose, liver enzymes activity (AST and ALT), malonic dialdehyde (MDA) as well as lipid hydroperoxide (LPO) concentrations in rats fed diet differing in type of dietary fat. Material and methods. Male Wistar rats were divided into six groups and fed the following diets: control AIN-93G diet contained soybean oil (O) and diets with modification of fat source: butter (B) and margarine (M). The experimental diets were supplemented with 1% of conjugated linoleic acid (O+CLA, B+CLA, M+CLA). After 21 days the blood was collected and lipid profile, glucose, liver enzymes, MDA as well as LPO were analyzed. Results. The dietary treatments had no significant effect on the body weight and liver weight of the animals. The concentrations of total cholesterol (TC) and LDL+VLDL cholesterol were unchanged. Both experimental factors (fat source and CLA) had a significant influence on the TAG and HDL levels. Margarine (M) significantly increased the TAG concentration, whereas CLA had a significant impact on the TAG reduction (M+CLA). Glucose level was significantly decreased in all groups fed diets supplemented with CLA. Serum ALT significantly increased in all CLA groups. Fat source had statistically significant influence on the MDA concentration. The LPO level was significantly elevated in all CLA groups. There was statistically significant interaction of experimental factors (fat source and CLA supplementation) on LPO level. Conclusions. Margarine had an adverse effect on the rat’s lipid profile. However, in the group fed with margarine, the addition of CLA decreased the concentration of TAG. Regardless of the type of the dietary fat, CLA supplementation increased the level of LPO in the blood serum of animals.http://wydawnictwa.pzh.gov.pl/roczniki_pzh/pobierz-artykul?id=1268conjugated linoleic aciddietary fatlipid profileliver enzymes activityoxidative stress markersrats |
| spellingShingle | Magdalena Franczyk-Żarów Edyta Kuś Renata B. Kostogrys Effect of conjugated linoleic acid and different type of dietary fat on serum lipid profile, liver enzymes activity and oxidative stress markers in Wistar rats Roczniki Panstwowego Zakladu Higieny conjugated linoleic acid dietary fat lipid profile liver enzymes activity oxidative stress markers rats |
| title | Effect of conjugated linoleic acid and different type of dietary fat on serum lipid profile, liver enzymes activity and oxidative stress markers in Wistar rats |
| title_full | Effect of conjugated linoleic acid and different type of dietary fat on serum lipid profile, liver enzymes activity and oxidative stress markers in Wistar rats |
| title_fullStr | Effect of conjugated linoleic acid and different type of dietary fat on serum lipid profile, liver enzymes activity and oxidative stress markers in Wistar rats |
| title_full_unstemmed | Effect of conjugated linoleic acid and different type of dietary fat on serum lipid profile, liver enzymes activity and oxidative stress markers in Wistar rats |
| title_short | Effect of conjugated linoleic acid and different type of dietary fat on serum lipid profile, liver enzymes activity and oxidative stress markers in Wistar rats |
| title_sort | effect of conjugated linoleic acid and different type of dietary fat on serum lipid profile liver enzymes activity and oxidative stress markers in wistar rats |
| topic | conjugated linoleic acid dietary fat lipid profile liver enzymes activity oxidative stress markers rats |
| url | http://wydawnictwa.pzh.gov.pl/roczniki_pzh/pobierz-artykul?id=1268 |
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