Prognostic Value of the Diversity of Nuclear Chromatin Compartments in Gynaecological Carcinomas
Statistical texture analysis of cancer cell nuclei stained for DNA has recently been used to develop a pan-cancer prognostic marker of chromatin heterogeneity. In this study, we instead analysed chromatin organisation by automatically quantifying the diversity of chromatin compartments in cancer cel...
| Main Authors: | , , , , |
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| Format: | Article |
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MDPI AG
2020-12-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/12/12/3838 |
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| author | Andreas Kleppe Fritz Albregtsen Jone Trovik Gunnar B. Kristensen Håvard E. Danielsen |
| author_facet | Andreas Kleppe Fritz Albregtsen Jone Trovik Gunnar B. Kristensen Håvard E. Danielsen |
| author_sort | Andreas Kleppe |
| collection | DOAJ |
| description | Statistical texture analysis of cancer cell nuclei stained for DNA has recently been used to develop a pan-cancer prognostic marker of chromatin heterogeneity. In this study, we instead analysed chromatin organisation by automatically quantifying the diversity of chromatin compartments in cancer cell nuclei. The aim was to investigate the prognostic value of such an assessment in relation to chromatin heterogeneity and as a potential supplement to pathological risk classifications in gynaecological carcinomas. The diversity was quantified by calculating the entropy of both chromatin compartment sizes and optical densities within compartments. We analysed a median of 281 nuclei (interquartile range (IQR), 273 to 289) from 246 ovarian carcinoma patients and a median of 997 nuclei (IQR, 502 to 1452) from 791 endometrial carcinoma patients. The prognostic value of the entropies and chromatin heterogeneity was moderately strongly correlated (<i>r</i> ranged from 0.68 to 0.73), but the novel marker was observed to provide additional prognostic information. In multivariable analysis with clinical and pathological markers, the hazard ratio associated with the novel marker was 2.1 (95% CI, 1.3 to 3.5) in ovarian carcinoma and 2.4 (95% CI, 1.5 to 3.9) in endometrial carcinoma. Integration with pathological risk classifications gave three risk groups with distinctly different prognoses. This suggests that the novel marker of diversity of chromatin compartments might possibly contribute to the selection of high-risk stage I ovarian carcinoma patients for adjuvant chemotherapy and low-risk endometrial carcinoma patients for less extensive surgery. |
| first_indexed | 2024-03-10T13:55:41Z |
| format | Article |
| id | doaj.art-e908c1d73632484cb870334f29c95b0c |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T13:55:41Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-e908c1d73632484cb870334f29c95b0c2023-11-21T01:38:26ZengMDPI AGCancers2072-66942020-12-011212383810.3390/cancers12123838Prognostic Value of the Diversity of Nuclear Chromatin Compartments in Gynaecological CarcinomasAndreas Kleppe0Fritz Albregtsen1Jone Trovik2Gunnar B. Kristensen3Håvard E. Danielsen4Institute for Cancer Genetics and Informatics, Oslo University Hospital, NO-0424 Oslo, NorwayInstitute for Cancer Genetics and Informatics, Oslo University Hospital, NO-0424 Oslo, NorwayDepartment of Obstetrics and Gynecology, Haukeland University Hospital, NO-5020 Bergen, NorwayInstitute for Cancer Genetics and Informatics, Oslo University Hospital, NO-0424 Oslo, NorwayInstitute for Cancer Genetics and Informatics, Oslo University Hospital, NO-0424 Oslo, NorwayStatistical texture analysis of cancer cell nuclei stained for DNA has recently been used to develop a pan-cancer prognostic marker of chromatin heterogeneity. In this study, we instead analysed chromatin organisation by automatically quantifying the diversity of chromatin compartments in cancer cell nuclei. The aim was to investigate the prognostic value of such an assessment in relation to chromatin heterogeneity and as a potential supplement to pathological risk classifications in gynaecological carcinomas. The diversity was quantified by calculating the entropy of both chromatin compartment sizes and optical densities within compartments. We analysed a median of 281 nuclei (interquartile range (IQR), 273 to 289) from 246 ovarian carcinoma patients and a median of 997 nuclei (IQR, 502 to 1452) from 791 endometrial carcinoma patients. The prognostic value of the entropies and chromatin heterogeneity was moderately strongly correlated (<i>r</i> ranged from 0.68 to 0.73), but the novel marker was observed to provide additional prognostic information. In multivariable analysis with clinical and pathological markers, the hazard ratio associated with the novel marker was 2.1 (95% CI, 1.3 to 3.5) in ovarian carcinoma and 2.4 (95% CI, 1.5 to 3.9) in endometrial carcinoma. Integration with pathological risk classifications gave three risk groups with distinctly different prognoses. This suggests that the novel marker of diversity of chromatin compartments might possibly contribute to the selection of high-risk stage I ovarian carcinoma patients for adjuvant chemotherapy and low-risk endometrial carcinoma patients for less extensive surgery.https://www.mdpi.com/2072-6694/12/12/3838chromatin compartmentschromatin organisationtexture analysisprognostic markerovarian cancerendometrial cancer |
| spellingShingle | Andreas Kleppe Fritz Albregtsen Jone Trovik Gunnar B. Kristensen Håvard E. Danielsen Prognostic Value of the Diversity of Nuclear Chromatin Compartments in Gynaecological Carcinomas Cancers chromatin compartments chromatin organisation texture analysis prognostic marker ovarian cancer endometrial cancer |
| title | Prognostic Value of the Diversity of Nuclear Chromatin Compartments in Gynaecological Carcinomas |
| title_full | Prognostic Value of the Diversity of Nuclear Chromatin Compartments in Gynaecological Carcinomas |
| title_fullStr | Prognostic Value of the Diversity of Nuclear Chromatin Compartments in Gynaecological Carcinomas |
| title_full_unstemmed | Prognostic Value of the Diversity of Nuclear Chromatin Compartments in Gynaecological Carcinomas |
| title_short | Prognostic Value of the Diversity of Nuclear Chromatin Compartments in Gynaecological Carcinomas |
| title_sort | prognostic value of the diversity of nuclear chromatin compartments in gynaecological carcinomas |
| topic | chromatin compartments chromatin organisation texture analysis prognostic marker ovarian cancer endometrial cancer |
| url | https://www.mdpi.com/2072-6694/12/12/3838 |
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