Extended in vivo transcriptomes of two ascoviruses with different tissue tropisms reveal alternative mechanisms for enhancing virus reproduction in hemolymph
Abstract Ascoviruses are large dsDNA viruses characterized by the extraordinary changes they induce in cellular pathogenesis and architecture whereby after nuclear lysis and extensive hypertrophy, each cell is cleaved into numerous vesicles for virion reproduction. However, the level of viral replic...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-08-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-95553-y |
| _version_ | 1819200375948836864 |
|---|---|
| author | Heba A. H. Zaghloul Robert H. Hice Peter Arensburger Dennis K. Bideshi Brian A. Federici |
| author_facet | Heba A. H. Zaghloul Robert H. Hice Peter Arensburger Dennis K. Bideshi Brian A. Federici |
| author_sort | Heba A. H. Zaghloul |
| collection | DOAJ |
| description | Abstract Ascoviruses are large dsDNA viruses characterized by the extraordinary changes they induce in cellular pathogenesis and architecture whereby after nuclear lysis and extensive hypertrophy, each cell is cleaved into numerous vesicles for virion reproduction. However, the level of viral replication and transcription in vesicles compared to other host tissues remains uncertain. Therefore, we applied RNA-Sequencing to compare the temporal transcriptome of Spodoptera frugiperda ascovirus (SfAV) and Trichoplusia ni ascovirus (TnAV) at 7, 14, and 21 days post-infection (dpi). We found most transcription occurred in viral vesicles, not in initial tissues infected, a remarkably novel reproduction mechanism compared to all other viruses and most other intracellular pathogens. Specifically, the highest level of viral gene expression occurred in hemolymph, for TnAV at 7 dpi, and SfAV at 14 dpi. Moreover, we found that host immune genes were partially down-regulated in hemolymph, where most viral replication occurred in highly dense accumulations of vesicles. |
| first_indexed | 2024-12-23T03:31:14Z |
| format | Article |
| id | doaj.art-e90ac31c1d04490aab7621f34d92506f |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-23T03:31:14Z |
| publishDate | 2021-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e90ac31c1d04490aab7621f34d92506f2022-12-21T18:01:41ZengNature PortfolioScientific Reports2045-23222021-08-0111111210.1038/s41598-021-95553-yExtended in vivo transcriptomes of two ascoviruses with different tissue tropisms reveal alternative mechanisms for enhancing virus reproduction in hemolymphHeba A. H. Zaghloul0Robert H. Hice1Peter Arensburger2Dennis K. Bideshi3Brian A. Federici4Interdepartmental Graduate Program in Microbiology and Institute for Integrative Genome BiologyInterdepartmental Graduate Program in Microbiology and Institute for Integrative Genome BiologyDepartment of Biological Sciences, California State Polytechnic University, PomonaDepartment of Biological Sciences, California Baptist UniversityInterdepartmental Graduate Program in Microbiology and Institute for Integrative Genome BiologyAbstract Ascoviruses are large dsDNA viruses characterized by the extraordinary changes they induce in cellular pathogenesis and architecture whereby after nuclear lysis and extensive hypertrophy, each cell is cleaved into numerous vesicles for virion reproduction. However, the level of viral replication and transcription in vesicles compared to other host tissues remains uncertain. Therefore, we applied RNA-Sequencing to compare the temporal transcriptome of Spodoptera frugiperda ascovirus (SfAV) and Trichoplusia ni ascovirus (TnAV) at 7, 14, and 21 days post-infection (dpi). We found most transcription occurred in viral vesicles, not in initial tissues infected, a remarkably novel reproduction mechanism compared to all other viruses and most other intracellular pathogens. Specifically, the highest level of viral gene expression occurred in hemolymph, for TnAV at 7 dpi, and SfAV at 14 dpi. Moreover, we found that host immune genes were partially down-regulated in hemolymph, where most viral replication occurred in highly dense accumulations of vesicles.https://doi.org/10.1038/s41598-021-95553-y |
| spellingShingle | Heba A. H. Zaghloul Robert H. Hice Peter Arensburger Dennis K. Bideshi Brian A. Federici Extended in vivo transcriptomes of two ascoviruses with different tissue tropisms reveal alternative mechanisms for enhancing virus reproduction in hemolymph Scientific Reports |
| title | Extended in vivo transcriptomes of two ascoviruses with different tissue tropisms reveal alternative mechanisms for enhancing virus reproduction in hemolymph |
| title_full | Extended in vivo transcriptomes of two ascoviruses with different tissue tropisms reveal alternative mechanisms for enhancing virus reproduction in hemolymph |
| title_fullStr | Extended in vivo transcriptomes of two ascoviruses with different tissue tropisms reveal alternative mechanisms for enhancing virus reproduction in hemolymph |
| title_full_unstemmed | Extended in vivo transcriptomes of two ascoviruses with different tissue tropisms reveal alternative mechanisms for enhancing virus reproduction in hemolymph |
| title_short | Extended in vivo transcriptomes of two ascoviruses with different tissue tropisms reveal alternative mechanisms for enhancing virus reproduction in hemolymph |
| title_sort | extended in vivo transcriptomes of two ascoviruses with different tissue tropisms reveal alternative mechanisms for enhancing virus reproduction in hemolymph |
| url | https://doi.org/10.1038/s41598-021-95553-y |
| work_keys_str_mv | AT hebaahzaghloul extendedinvivotranscriptomesoftwoascoviruseswithdifferenttissuetropismsrevealalternativemechanismsforenhancingvirusreproductioninhemolymph AT roberthhice extendedinvivotranscriptomesoftwoascoviruseswithdifferenttissuetropismsrevealalternativemechanismsforenhancingvirusreproductioninhemolymph AT peterarensburger extendedinvivotranscriptomesoftwoascoviruseswithdifferenttissuetropismsrevealalternativemechanismsforenhancingvirusreproductioninhemolymph AT denniskbideshi extendedinvivotranscriptomesoftwoascoviruseswithdifferenttissuetropismsrevealalternativemechanismsforenhancingvirusreproductioninhemolymph AT brianafederici extendedinvivotranscriptomesoftwoascoviruseswithdifferenttissuetropismsrevealalternativemechanismsforenhancingvirusreproductioninhemolymph |