Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock
Abstract Background Leptospirosis is caused by pathogenic spirochetes of the genus Leptospira spp. This zoonotic disease is distributed globally and affects domestic animals, including cattle. Leptospira interrogans serogroup Sejroe serovar Hardjo and Leptospira borgpetersenii serogroup Sejroe serov...
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| Format: | Article |
| Language: | English |
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BMC
2017-06-01
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| Series: | BMC Veterinary Research |
| Online Access: | http://link.springer.com/article/10.1186/s12917-017-1081-9 |
| _version_ | 1818513525965848576 |
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| author | Maria Raquel V. Cosate Tetsu Sakamoto Tiago Antônio de Oliveira Mendes Élvio C. Moreira Carlos G. Regis da Silva Bruno S. A. F. Brasil Camila S. F. Oliveira Vasco Ariston de Azevedo José Miguel Ortega Rômulo C. Leite João Paulo Haddad |
| author_facet | Maria Raquel V. Cosate Tetsu Sakamoto Tiago Antônio de Oliveira Mendes Élvio C. Moreira Carlos G. Regis da Silva Bruno S. A. F. Brasil Camila S. F. Oliveira Vasco Ariston de Azevedo José Miguel Ortega Rômulo C. Leite João Paulo Haddad |
| author_sort | Maria Raquel V. Cosate |
| collection | DOAJ |
| description | Abstract Background Leptospirosis is caused by pathogenic spirochetes of the genus Leptospira spp. This zoonotic disease is distributed globally and affects domestic animals, including cattle. Leptospira interrogans serogroup Sejroe serovar Hardjo and Leptospira borgpetersenii serogroup Sejroe serovar Hardjo remain important species associated with this reproductive disease in livestock production. Previous studies on Brazilian livestock have reported that L. interrogans serovar Hardjo is the most prevalent leptospiral agent in this country and is related to clinical signs of leptospirosis, which lead to economic losses in production. Here, we described the isolation of three clinical strains (Norma, Lagoa and Bolivia) obtained from leptospirosis outbreaks that occurred in Minas Gerais state in 1994 and 2008. Results Serological and molecular typing using housekeeping (secY and 16SrRNA) and rfb locus (ORF22 and ORF36) genes were applied for the identification and comparative analysis of Leptospira spp. Our results identified the three isolates as L. interrogans serogroup Sejroe serovar Hardjo and confirmed the occurrence of this bacterial strain in Brazilian livestock. Genetic analysis using ORF22 and ORF36 grouped the Leptospira into serogroup Sejroe and subtype Hardjoprajitno. Genetic approaches were also applied to compare distinct serovars of L. interrogans strains by verifying the copy numbers of the IS1500 and IS1533 insertion sequences (ISs). The IS1500 copy number varied among the analyzed L. interrogans strains. Conclusion This study provides evidence that L. interrogans serogroup Sejroe serovar Hardjo subtype Hardjoprajitno causes bovine leptospirosis in Brazilian production. The molecular results suggested that rfb locus (ORF22 and ORF36) could improve epidemiological studies by allowing the identification of Leptospira spp. at the serogroup level. Additionally, the IS1500 and IS1533 IS copy number analysis suggested distinct genomic features among closely related leptospiral strains. |
| first_indexed | 2024-12-11T00:02:40Z |
| format | Article |
| id | doaj.art-e90bd2ca4c914800a2fb6f6733eea0b8 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-11T00:02:40Z |
| publishDate | 2017-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-e90bd2ca4c914800a2fb6f6733eea0b82022-12-22T01:28:25ZengBMCBMC Veterinary Research1746-61482017-06-0113111210.1186/s12917-017-1081-9Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestockMaria Raquel V. Cosate0Tetsu Sakamoto1Tiago Antônio de Oliveira Mendes2Élvio C. Moreira3Carlos G. Regis da Silva4Bruno S. A. F. Brasil5Camila S. F. Oliveira6Vasco Ariston de Azevedo7José Miguel Ortega8Rômulo C. Leite9João Paulo Haddad10Instituto de Ciências Biológicas, Departamento de Bioquímica e Imunologia, Universidade Federal de Minas GeraisInstituto de Ciências Biológicas, Departamento de Bioquímica e Imunologia, Universidade Federal de Minas GeraisInstituto de Ciências Biológicas, Departamento de Bioquímica e Imunologia, Universidade Federal de Minas GeraisDepartamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas GeraisLaboratório de Biologia Parasitária, Centro de Pesquisas Gonçalo Moniz- FiocruzEmbrapa AgroenergiaDepartamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas GeraisUniversidade Federal de Minas Gerais, Departamento de Genética - Instituto de Ciências Biol’ogicas de Minas GeraisInstituto de Ciências Biológicas, Departamento de Bioquímica e Imunologia, Universidade Federal de Minas GeraisDepartamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas GeraisDepartamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas GeraisAbstract Background Leptospirosis is caused by pathogenic spirochetes of the genus Leptospira spp. This zoonotic disease is distributed globally and affects domestic animals, including cattle. Leptospira interrogans serogroup Sejroe serovar Hardjo and Leptospira borgpetersenii serogroup Sejroe serovar Hardjo remain important species associated with this reproductive disease in livestock production. Previous studies on Brazilian livestock have reported that L. interrogans serovar Hardjo is the most prevalent leptospiral agent in this country and is related to clinical signs of leptospirosis, which lead to economic losses in production. Here, we described the isolation of three clinical strains (Norma, Lagoa and Bolivia) obtained from leptospirosis outbreaks that occurred in Minas Gerais state in 1994 and 2008. Results Serological and molecular typing using housekeeping (secY and 16SrRNA) and rfb locus (ORF22 and ORF36) genes were applied for the identification and comparative analysis of Leptospira spp. Our results identified the three isolates as L. interrogans serogroup Sejroe serovar Hardjo and confirmed the occurrence of this bacterial strain in Brazilian livestock. Genetic analysis using ORF22 and ORF36 grouped the Leptospira into serogroup Sejroe and subtype Hardjoprajitno. Genetic approaches were also applied to compare distinct serovars of L. interrogans strains by verifying the copy numbers of the IS1500 and IS1533 insertion sequences (ISs). The IS1500 copy number varied among the analyzed L. interrogans strains. Conclusion This study provides evidence that L. interrogans serogroup Sejroe serovar Hardjo subtype Hardjoprajitno causes bovine leptospirosis in Brazilian production. The molecular results suggested that rfb locus (ORF22 and ORF36) could improve epidemiological studies by allowing the identification of Leptospira spp. at the serogroup level. Additionally, the IS1500 and IS1533 IS copy number analysis suggested distinct genomic features among closely related leptospiral strains.http://link.springer.com/article/10.1186/s12917-017-1081-9 |
| spellingShingle | Maria Raquel V. Cosate Tetsu Sakamoto Tiago Antônio de Oliveira Mendes Élvio C. Moreira Carlos G. Regis da Silva Bruno S. A. F. Brasil Camila S. F. Oliveira Vasco Ariston de Azevedo José Miguel Ortega Rômulo C. Leite João Paulo Haddad Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock BMC Veterinary Research |
| title | Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock |
| title_full | Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock |
| title_fullStr | Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock |
| title_full_unstemmed | Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock |
| title_short | Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock |
| title_sort | molecular typing of leptospira interrogans serovar hardjo isolates from leptospirosis outbreaks in brazilian livestock |
| url | http://link.springer.com/article/10.1186/s12917-017-1081-9 |
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