MiR-301a Promotes Cell Proliferation by Repressing PTEN in Renal Cell Carcinoma
Jun Li,1,* Donggen Jiang,1,* Qian Zhang,2,* Shubin Peng,3 Guolong Liao,1 Xiangwei Yang,1 Jiani Tang,1 Haiyun Xiong,1 Jun Pang1 1Department of Urology, Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, People’s Republic of China; 2Department of Rehabilitation Medicine, S...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-06-01
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| Series: | Cancer Management and Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/mir-301a-promotes-cell-proliferation-by-repressing-pten-in-renal-cell--peer-reviewed-article-CMAR |
| _version_ | 1818521359599271936 |
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| author | Li J Jiang D Zhang Q Peng S Liao G Yang X Tang J Xiong H Pang J |
| author_facet | Li J Jiang D Zhang Q Peng S Liao G Yang X Tang J Xiong H Pang J |
| author_sort | Li J |
| collection | DOAJ |
| description | Jun Li,1,* Donggen Jiang,1,* Qian Zhang,2,* Shubin Peng,3 Guolong Liao,1 Xiangwei Yang,1 Jiani Tang,1 Haiyun Xiong,1 Jun Pang1 1Department of Urology, Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, People’s Republic of China; 2Department of Rehabilitation Medicine, Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, People’s Republic of China; 3Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jun Pang; Haiyun XiongDepartment of Urology, Seventh Affiliated Hospital, Sun Yat-Sen University, 628 Zhenyuan Road, Shenzhen 518107, People’s Republic of ChinaTel +86-18802044177; +86-18922869986Email pangjun2@mail.sysu.edu.cn; xionghy7@mail.sysu.edu.cnObjective: Renal cell carcinoma (RCC) displays an increasing incidence and mortality rate worldwide in recent years. More and more evidence demonstrated microRNAs function as positive or negative regulatory factors in many cancers, while the role of miR-301a in RCC is still unclear.Material and Methods: The expression and clinical significance of miR-301a were assessed via bioinformatic software on open microarray datasets of the Cancer Genome Atlas (TCGA) and then confirmed by quantitative real-time PCR (qRT-PCR) in RCC cell lines. Loss of function assays were performed in RCC cell lines both in vitro and in vivo. Cell Counting Kit-8 (CCK-8), flow cytometry, luciferase reporter assays, Western blotting, and immunohistochemistry were employed to explore the mechanisms of the effect of miR-301a on RCC.Results: By analyzing RCC clinical specimens and cell lines, we found a uniform increased miR-301a in expression in comparison with normal renal tissue or normal human proximal tubule epithelial cell line (HK-2). In addition, miR-301a upregulation correlated advanced stage and poor prognosis of clear cell RCC (ccRCC). Anti-miR-301a could inhibit growth and cell cycle G1/S transition in RCC cell lines. Moreover, we found that PTEN was identified as a direct target of miR-301a that might partially interrupt miR-301a-induced G1/S transition. Importantly, nude-mouse models revealed that knockdown of miR-301a delayed tumor growth.Conclusion: These results indicate that miR-301a functions as a tumor-promoting miRNA through regulating PTEN expression, representing a novel therapeutic target for RCC.Keywords: miR-301a, PTEN, renal cell carcinoma, proliferation, G1/S transition |
| first_indexed | 2024-12-11T01:49:58Z |
| format | Article |
| id | doaj.art-e90de955e05b4341b9806a3557ea4b27 |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-11T01:49:58Z |
| publishDate | 2020-06-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-e90de955e05b4341b9806a3557ea4b272022-12-22T01:24:46ZengDove Medical PressCancer Management and Research1179-13222020-06-01Volume 124309432054405MiR-301a Promotes Cell Proliferation by Repressing PTEN in Renal Cell CarcinomaLi JJiang DZhang QPeng SLiao GYang XTang JXiong HPang JJun Li,1,* Donggen Jiang,1,* Qian Zhang,2,* Shubin Peng,3 Guolong Liao,1 Xiangwei Yang,1 Jiani Tang,1 Haiyun Xiong,1 Jun Pang1 1Department of Urology, Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, People’s Republic of China; 2Department of Rehabilitation Medicine, Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, People’s Republic of China; 3Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jun Pang; Haiyun XiongDepartment of Urology, Seventh Affiliated Hospital, Sun Yat-Sen University, 628 Zhenyuan Road, Shenzhen 518107, People’s Republic of ChinaTel +86-18802044177; +86-18922869986Email pangjun2@mail.sysu.edu.cn; xionghy7@mail.sysu.edu.cnObjective: Renal cell carcinoma (RCC) displays an increasing incidence and mortality rate worldwide in recent years. More and more evidence demonstrated microRNAs function as positive or negative regulatory factors in many cancers, while the role of miR-301a in RCC is still unclear.Material and Methods: The expression and clinical significance of miR-301a were assessed via bioinformatic software on open microarray datasets of the Cancer Genome Atlas (TCGA) and then confirmed by quantitative real-time PCR (qRT-PCR) in RCC cell lines. Loss of function assays were performed in RCC cell lines both in vitro and in vivo. Cell Counting Kit-8 (CCK-8), flow cytometry, luciferase reporter assays, Western blotting, and immunohistochemistry were employed to explore the mechanisms of the effect of miR-301a on RCC.Results: By analyzing RCC clinical specimens and cell lines, we found a uniform increased miR-301a in expression in comparison with normal renal tissue or normal human proximal tubule epithelial cell line (HK-2). In addition, miR-301a upregulation correlated advanced stage and poor prognosis of clear cell RCC (ccRCC). Anti-miR-301a could inhibit growth and cell cycle G1/S transition in RCC cell lines. Moreover, we found that PTEN was identified as a direct target of miR-301a that might partially interrupt miR-301a-induced G1/S transition. Importantly, nude-mouse models revealed that knockdown of miR-301a delayed tumor growth.Conclusion: These results indicate that miR-301a functions as a tumor-promoting miRNA through regulating PTEN expression, representing a novel therapeutic target for RCC.Keywords: miR-301a, PTEN, renal cell carcinoma, proliferation, G1/S transitionhttps://www.dovepress.com/mir-301a-promotes-cell-proliferation-by-repressing-pten-in-renal-cell--peer-reviewed-article-CMARmir-301aptenrenal cell carcinomaproliferationg1/s transition |
| spellingShingle | Li J Jiang D Zhang Q Peng S Liao G Yang X Tang J Xiong H Pang J MiR-301a Promotes Cell Proliferation by Repressing PTEN in Renal Cell Carcinoma Cancer Management and Research mir-301a pten renal cell carcinoma proliferation g1/s transition |
| title | MiR-301a Promotes Cell Proliferation by Repressing PTEN in Renal Cell Carcinoma |
| title_full | MiR-301a Promotes Cell Proliferation by Repressing PTEN in Renal Cell Carcinoma |
| title_fullStr | MiR-301a Promotes Cell Proliferation by Repressing PTEN in Renal Cell Carcinoma |
| title_full_unstemmed | MiR-301a Promotes Cell Proliferation by Repressing PTEN in Renal Cell Carcinoma |
| title_short | MiR-301a Promotes Cell Proliferation by Repressing PTEN in Renal Cell Carcinoma |
| title_sort | mir 301a promotes cell proliferation by repressing pten in renal cell carcinoma |
| topic | mir-301a pten renal cell carcinoma proliferation g1/s transition |
| url | https://www.dovepress.com/mir-301a-promotes-cell-proliferation-by-repressing-pten-in-renal-cell--peer-reviewed-article-CMAR |
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