Development of an extrusion-based 3D-printing strategy for clustering of human neural progenitor cells
3D bioprinting offers a simplified solution for the engineering of complex tissue parts for in-vitro drug discovery or, in-vivo implantation. However, significant amount of challenges exist in 3D bioprinting of neural tissues, as these are sensitive cell types to handle via extrusion bioprinting tec...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022035381 |
| _version_ | 1797960932488380416 |
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| author | Ines Bilkic Diana Sotelo Stephanie Anujarerat Nickolas R. Ortiz Matthew Alonzo Raven El Khoury Carla C. Loyola Binata Joddar |
| author_facet | Ines Bilkic Diana Sotelo Stephanie Anujarerat Nickolas R. Ortiz Matthew Alonzo Raven El Khoury Carla C. Loyola Binata Joddar |
| author_sort | Ines Bilkic |
| collection | DOAJ |
| description | 3D bioprinting offers a simplified solution for the engineering of complex tissue parts for in-vitro drug discovery or, in-vivo implantation. However, significant amount of challenges exist in 3D bioprinting of neural tissues, as these are sensitive cell types to handle via extrusion bioprinting techniques. We assessed the feasibility of bioprinting human neural progenitor cells (NPCs) in 3D hydrogel lattices using a fibrinogen-alginate-chitosan bioink, previously optimized for neural-cell growth, and subsequently modified for structural support during extrusion printing, in this study. The original bioink used in this study was made by adding optimized amounts of high- and medium-viscosity alginate to the fibrinogen-chitosan-based bioink and making it extrudable under shear pressure. The mechanically robust 3D constructs promoted NPC cluster formation and maintained their morphology and viability during the entire culture period. This strategy may be useful for co-culturing of NPCs along with other cell types such as cardiac, vascular, and other cells during 3D bioprinting. |
| first_indexed | 2024-04-11T00:51:41Z |
| format | Article |
| id | doaj.art-e90eac908ddd4ff39519734a9a0076db |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-11T00:51:41Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-e90eac908ddd4ff39519734a9a0076db2023-01-05T08:39:35ZengElsevierHeliyon2405-84402022-12-01812e12250Development of an extrusion-based 3D-printing strategy for clustering of human neural progenitor cellsInes Bilkic0Diana Sotelo1Stephanie Anujarerat2Nickolas R. Ortiz3Matthew Alonzo4Raven El Khoury5Carla C. Loyola6Binata Joddar7Department of Chemical Engineering and Materials Research Laboratory, University of California, Santa Barbara, CA 93106, USA; Inspired Materials and Stem-Cell Based Tissue Engineering Laboratory (IMSTEL), The University of Texas at El Paso, El Paso, TX, 79968, USAInspired Materials and Stem-Cell Based Tissue Engineering Laboratory (IMSTEL), The University of Texas at El Paso, El Paso, TX, 79968, USA; Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX, 79968, USADepartment of Chemical Engineering and Materials Research Laboratory, University of California, Santa Barbara, CA 93106, USA; Inspired Materials and Stem-Cell Based Tissue Engineering Laboratory (IMSTEL), The University of Texas at El Paso, El Paso, TX, 79968, USAInspired Materials and Stem-Cell Based Tissue Engineering Laboratory (IMSTEL), The University of Texas at El Paso, El Paso, TX, 79968, USA; Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX, 79968, USAInspired Materials and Stem-Cell Based Tissue Engineering Laboratory (IMSTEL), The University of Texas at El Paso, El Paso, TX, 79968, USA; Department of Metallurgical, Materials, and Biomedical Engineering, M201 Engineering, The University of Texas at El Paso, 500 W. University Avenue, El Paso, TX, 79968, USAInspired Materials and Stem-Cell Based Tissue Engineering Laboratory (IMSTEL), The University of Texas at El Paso, El Paso, TX, 79968, USA; Department of Metallurgical, Materials, and Biomedical Engineering, M201 Engineering, The University of Texas at El Paso, 500 W. University Avenue, El Paso, TX, 79968, USAInspired Materials and Stem-Cell Based Tissue Engineering Laboratory (IMSTEL), The University of Texas at El Paso, El Paso, TX, 79968, USA; Department of Metallurgical, Materials, and Biomedical Engineering, M201 Engineering, The University of Texas at El Paso, 500 W. University Avenue, El Paso, TX, 79968, USAInspired Materials and Stem-Cell Based Tissue Engineering Laboratory (IMSTEL), The University of Texas at El Paso, El Paso, TX, 79968, USA; Department of Metallurgical, Materials, and Biomedical Engineering, M201 Engineering, The University of Texas at El Paso, 500 W. University Avenue, El Paso, TX, 79968, USA; Department of Biological Sciences, The University of Texas at El Paso, El Paso, TX, 79968, USA; Border Biomedical Research Center, The University of Texas at El Paso, 500 W. University Avenue, El Paso, TX, 79968, USA; Corresponding author.3D bioprinting offers a simplified solution for the engineering of complex tissue parts for in-vitro drug discovery or, in-vivo implantation. However, significant amount of challenges exist in 3D bioprinting of neural tissues, as these are sensitive cell types to handle via extrusion bioprinting techniques. We assessed the feasibility of bioprinting human neural progenitor cells (NPCs) in 3D hydrogel lattices using a fibrinogen-alginate-chitosan bioink, previously optimized for neural-cell growth, and subsequently modified for structural support during extrusion printing, in this study. The original bioink used in this study was made by adding optimized amounts of high- and medium-viscosity alginate to the fibrinogen-chitosan-based bioink and making it extrudable under shear pressure. The mechanically robust 3D constructs promoted NPC cluster formation and maintained their morphology and viability during the entire culture period. This strategy may be useful for co-culturing of NPCs along with other cell types such as cardiac, vascular, and other cells during 3D bioprinting.http://www.sciencedirect.com/science/article/pii/S2405844022035381Neural tissue engineeringBiofabricationBioinksNeural progenitor cellsProcess optimizationRheology |
| spellingShingle | Ines Bilkic Diana Sotelo Stephanie Anujarerat Nickolas R. Ortiz Matthew Alonzo Raven El Khoury Carla C. Loyola Binata Joddar Development of an extrusion-based 3D-printing strategy for clustering of human neural progenitor cells Heliyon Neural tissue engineering Biofabrication Bioinks Neural progenitor cells Process optimization Rheology |
| title | Development of an extrusion-based 3D-printing strategy for clustering of human neural progenitor cells |
| title_full | Development of an extrusion-based 3D-printing strategy for clustering of human neural progenitor cells |
| title_fullStr | Development of an extrusion-based 3D-printing strategy for clustering of human neural progenitor cells |
| title_full_unstemmed | Development of an extrusion-based 3D-printing strategy for clustering of human neural progenitor cells |
| title_short | Development of an extrusion-based 3D-printing strategy for clustering of human neural progenitor cells |
| title_sort | development of an extrusion based 3d printing strategy for clustering of human neural progenitor cells |
| topic | Neural tissue engineering Biofabrication Bioinks Neural progenitor cells Process optimization Rheology |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022035381 |
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