TaRac6 Is a Potential Susceptibility Factor by Regulating the ROS Burst Negatively in the Wheat–Puccinia striiformis f. sp. tritici Interaction
Rac/Rop proteins play important roles in the regulation of cell growth and plant defense responses. However, the function of Rac/Rop proteins in wheat remains largely unknown. In this study, a small G protein gene, designated as TaRac6, was characterized from wheat (Triticum aestivum) in response to...
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.00716/full |
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| author | Qiong Zhang Xinmei Zhang Rui Zhuang Zetong Wei Weixue Shu Xiaojie Wang Zhensheng Kang |
| author_facet | Qiong Zhang Xinmei Zhang Rui Zhuang Zetong Wei Weixue Shu Xiaojie Wang Zhensheng Kang |
| author_sort | Qiong Zhang |
| collection | DOAJ |
| description | Rac/Rop proteins play important roles in the regulation of cell growth and plant defense responses. However, the function of Rac/Rop proteins in wheat remains largely unknown. In this study, a small G protein gene, designated as TaRac6, was characterized from wheat (Triticum aestivum) in response to Puccinia striiformis f. sp. tritici (Pst) and was found to be highly homologous to the Rac proteins identified in other plant species. Transient expression analyses of the TaRac6-GFP fusion protein in Nicotiana benthamiana leaves showed that TaRac6 was localized in the whole cell. Furthermore, transient expression of TaRac6 inhibited Bax-triggered plant cell death (PCD) in N. benthamiana. Transcript accumulation of TaRac6 was increased at 24 h post-inoculation (hpi) in the compatible interaction between wheat and Pst, while it was not induced in an incompatible interaction. More importantly, silencing of TaRac6 by virus induced gene silencing (VIGS) enhanced the resistance of wheat (Suwon 11) to Pst (CYR31) by producing fewer uredinia. Histological observations revealed that the hypha growth of Pst was markedly inhibited along with more H2O2 generated in the TaRac6-silenced leaves in response to Pst. Moreover, transcript levels of TaCAT were significantly down-regulated, while those of TaSOD and TaNOX were significantly up-regulated. These results suggest that TaRac6 functions as a potential susceptibility factor, which negatively regulate the reactive oxygen species (ROS) burst in the wheat–Pst interaction. |
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| last_indexed | 2024-12-23T10:38:22Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-e913c8bb617b452c8ea7136507987ebc2022-12-21T17:50:14ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-06-011110.3389/fpls.2020.00716507037TaRac6 Is a Potential Susceptibility Factor by Regulating the ROS Burst Negatively in the Wheat–Puccinia striiformis f. sp. tritici InteractionQiong Zhang0Xinmei Zhang1Rui Zhuang2Zetong Wei3Weixue Shu4Xiaojie Wang5Zhensheng Kang6State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, ChinaCollege of Life Sciences, Northwest A&F University, Yangling, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, ChinaRac/Rop proteins play important roles in the regulation of cell growth and plant defense responses. However, the function of Rac/Rop proteins in wheat remains largely unknown. In this study, a small G protein gene, designated as TaRac6, was characterized from wheat (Triticum aestivum) in response to Puccinia striiformis f. sp. tritici (Pst) and was found to be highly homologous to the Rac proteins identified in other plant species. Transient expression analyses of the TaRac6-GFP fusion protein in Nicotiana benthamiana leaves showed that TaRac6 was localized in the whole cell. Furthermore, transient expression of TaRac6 inhibited Bax-triggered plant cell death (PCD) in N. benthamiana. Transcript accumulation of TaRac6 was increased at 24 h post-inoculation (hpi) in the compatible interaction between wheat and Pst, while it was not induced in an incompatible interaction. More importantly, silencing of TaRac6 by virus induced gene silencing (VIGS) enhanced the resistance of wheat (Suwon 11) to Pst (CYR31) by producing fewer uredinia. Histological observations revealed that the hypha growth of Pst was markedly inhibited along with more H2O2 generated in the TaRac6-silenced leaves in response to Pst. Moreover, transcript levels of TaCAT were significantly down-regulated, while those of TaSOD and TaNOX were significantly up-regulated. These results suggest that TaRac6 functions as a potential susceptibility factor, which negatively regulate the reactive oxygen species (ROS) burst in the wheat–Pst interaction.https://www.frontiersin.org/article/10.3389/fpls.2020.00716/fullTaRac6wheatPuccinia striiformis f. sp. triticireactive oxygen speciessusceptibility factor |
| spellingShingle | Qiong Zhang Xinmei Zhang Rui Zhuang Zetong Wei Weixue Shu Xiaojie Wang Zhensheng Kang TaRac6 Is a Potential Susceptibility Factor by Regulating the ROS Burst Negatively in the Wheat–Puccinia striiformis f. sp. tritici Interaction Frontiers in Plant Science TaRac6 wheat Puccinia striiformis f. sp. tritici reactive oxygen species susceptibility factor |
| title | TaRac6 Is a Potential Susceptibility Factor by Regulating the ROS Burst Negatively in the Wheat–Puccinia striiformis f. sp. tritici Interaction |
| title_full | TaRac6 Is a Potential Susceptibility Factor by Regulating the ROS Burst Negatively in the Wheat–Puccinia striiformis f. sp. tritici Interaction |
| title_fullStr | TaRac6 Is a Potential Susceptibility Factor by Regulating the ROS Burst Negatively in the Wheat–Puccinia striiformis f. sp. tritici Interaction |
| title_full_unstemmed | TaRac6 Is a Potential Susceptibility Factor by Regulating the ROS Burst Negatively in the Wheat–Puccinia striiformis f. sp. tritici Interaction |
| title_short | TaRac6 Is a Potential Susceptibility Factor by Regulating the ROS Burst Negatively in the Wheat–Puccinia striiformis f. sp. tritici Interaction |
| title_sort | tarac6 is a potential susceptibility factor by regulating the ros burst negatively in the wheat puccinia striiformis f sp tritici interaction |
| topic | TaRac6 wheat Puccinia striiformis f. sp. tritici reactive oxygen species susceptibility factor |
| url | https://www.frontiersin.org/article/10.3389/fpls.2020.00716/full |
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