Protective immunity to Japanese encephalitis virus associated with anti-NS1 antibodies in a mouse model
<p>Abstract</p> <p>Background</p> <p>Japanese encephalitis virus (JEV) is a major mosquito-borne pathogen that causes viral encephalitis throughout Asia. Vaccination with an inactive JEV particle or attenuated virus is an efficient preventative measure for controlling i...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-07-01
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| Series: | Virology Journal |
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| Online Access: | http://www.virologyj.com/content/9/1/135 |
| _version_ | 1811283406818902016 |
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| author | Li Yize Counor Dorian Lu Peng Duong Veasna Yu Yongxin Deubel Vincent |
| author_facet | Li Yize Counor Dorian Lu Peng Duong Veasna Yu Yongxin Deubel Vincent |
| author_sort | Li Yize |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Japanese encephalitis virus (JEV) is a major mosquito-borne pathogen that causes viral encephalitis throughout Asia. Vaccination with an inactive JEV particle or attenuated virus is an efficient preventative measure for controlling infection. Flavivirus NS1 protein is a glycoprotein secreted during viral replication that plays multiple roles in the viral life cycle and pathogenesis. Utilizing JEV NS1 as an antigen in viral vectors induces a limited protective immune response against infection. Previous studies using <it>E. coli</it>-expressed JEV NS1 to immunize mice induced protection against lethal challenge; however, the protection mechanism through cellular and humoral immune responses was not described.</p> <p>Results</p> <p>JEV NS1 was expressed in and purified from <it>Drosophila</it> S2 cells in a native glycosylated multimeric form, which induced T-cell and antibody responses in immunized C3H/HeN mice. Mice vaccinated with 1 μg NS1 with or without water-in-oil adjuvant were partially protected against viral challenge and higher protection was observed in mice with higher antibody titers. IgG1 was preferentially elicited by an adjuvanted NS1 protein, whereas a larger load of IFN-γ was produced in splenocytes from mice immunized with aqueous NS1. Mice that passively received anti-NS1 mouse polyclonal immune sera were protected, and this phenomenon was dose-dependent, whereas protection was low or delayed after the passive transfer of anti-NS1 MAbs.</p> <p>Conclusion</p> <p>The purified NS1 subunit induced protective immunity in relation with anti-NS1 IgG1 antibodies. NS1 protein efficiently stimulated Th1-cell proliferation and IFN-γ production. Protection against lethal challenge was elicited by passive transfer of anti-NS1 antisera, suggesting that anti-NS1 antibodies play a substantial role in anti-viral immunity</p> |
| first_indexed | 2024-04-13T02:10:56Z |
| format | Article |
| id | doaj.art-e91a1085cfb74e0491d961d2b6498f16 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-04-13T02:10:56Z |
| publishDate | 2012-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-e91a1085cfb74e0491d961d2b6498f162022-12-22T03:07:17ZengBMCVirology Journal1743-422X2012-07-019113510.1186/1743-422X-9-135Protective immunity to Japanese encephalitis virus associated with anti-NS1 antibodies in a mouse modelLi YizeCounor DorianLu PengDuong VeasnaYu YongxinDeubel Vincent<p>Abstract</p> <p>Background</p> <p>Japanese encephalitis virus (JEV) is a major mosquito-borne pathogen that causes viral encephalitis throughout Asia. Vaccination with an inactive JEV particle or attenuated virus is an efficient preventative measure for controlling infection. Flavivirus NS1 protein is a glycoprotein secreted during viral replication that plays multiple roles in the viral life cycle and pathogenesis. Utilizing JEV NS1 as an antigen in viral vectors induces a limited protective immune response against infection. Previous studies using <it>E. coli</it>-expressed JEV NS1 to immunize mice induced protection against lethal challenge; however, the protection mechanism through cellular and humoral immune responses was not described.</p> <p>Results</p> <p>JEV NS1 was expressed in and purified from <it>Drosophila</it> S2 cells in a native glycosylated multimeric form, which induced T-cell and antibody responses in immunized C3H/HeN mice. Mice vaccinated with 1 μg NS1 with or without water-in-oil adjuvant were partially protected against viral challenge and higher protection was observed in mice with higher antibody titers. IgG1 was preferentially elicited by an adjuvanted NS1 protein, whereas a larger load of IFN-γ was produced in splenocytes from mice immunized with aqueous NS1. Mice that passively received anti-NS1 mouse polyclonal immune sera were protected, and this phenomenon was dose-dependent, whereas protection was low or delayed after the passive transfer of anti-NS1 MAbs.</p> <p>Conclusion</p> <p>The purified NS1 subunit induced protective immunity in relation with anti-NS1 IgG1 antibodies. NS1 protein efficiently stimulated Th1-cell proliferation and IFN-γ production. Protection against lethal challenge was elicited by passive transfer of anti-NS1 antisera, suggesting that anti-NS1 antibodies play a substantial role in anti-viral immunity</p>http://www.virologyj.com/content/9/1/135Japanese encephalitis virusT-cell response, AntibodiesMonoclonal antibodiesNS1 proteinMouse model |
| spellingShingle | Li Yize Counor Dorian Lu Peng Duong Veasna Yu Yongxin Deubel Vincent Protective immunity to Japanese encephalitis virus associated with anti-NS1 antibodies in a mouse model Virology Journal Japanese encephalitis virus T-cell response, Antibodies Monoclonal antibodies NS1 protein Mouse model |
| title | Protective immunity to Japanese encephalitis virus associated with anti-NS1 antibodies in a mouse model |
| title_full | Protective immunity to Japanese encephalitis virus associated with anti-NS1 antibodies in a mouse model |
| title_fullStr | Protective immunity to Japanese encephalitis virus associated with anti-NS1 antibodies in a mouse model |
| title_full_unstemmed | Protective immunity to Japanese encephalitis virus associated with anti-NS1 antibodies in a mouse model |
| title_short | Protective immunity to Japanese encephalitis virus associated with anti-NS1 antibodies in a mouse model |
| title_sort | protective immunity to japanese encephalitis virus associated with anti ns1 antibodies in a mouse model |
| topic | Japanese encephalitis virus T-cell response, Antibodies Monoclonal antibodies NS1 protein Mouse model |
| url | http://www.virologyj.com/content/9/1/135 |
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