Resumption of Cyclic Ovarian Activity by Herbal Preparation AyuFertin in Bulgarian Murrah Buffaloes at Early Postpartum
This study evaluates the phytochemical composition and ability of herbal preparation AyuFertin, supplemented shortly after calving, to resume the cyclic ovarian activity in postpartum Bulgarian Murrah buffaloes. In total, 13 healthy Bulgarian Murrah buffaloes at the age of 4–10 years, calved in Marc...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/11/2/420 |
| _version_ | 1797413733265309696 |
|---|---|
| author | Yordanka Ilieva Nasko Vasilev Ivan Fasulkov Pencho Penchev Desislava Abadjieva Vanya Mladenova Ayla Ilyazova Dasha Mihaylova Stanimir Yotov Elena Kistanova |
| author_facet | Yordanka Ilieva Nasko Vasilev Ivan Fasulkov Pencho Penchev Desislava Abadjieva Vanya Mladenova Ayla Ilyazova Dasha Mihaylova Stanimir Yotov Elena Kistanova |
| author_sort | Yordanka Ilieva |
| collection | DOAJ |
| description | This study evaluates the phytochemical composition and ability of herbal preparation AyuFertin, supplemented shortly after calving, to resume the cyclic ovarian activity in postpartum Bulgarian Murrah buffaloes. In total, 13 healthy Bulgarian Murrah buffaloes at the age of 4–10 years, calved in March–April 2019 were involved in the experiment. Seven experimental buffaloes were treated individually per os with AyuFertin according to producer instruction. All animals were subjected to regular ultrasound examination of ovaries. GC-MS analysis of fatty acids composition and HPLC-DAD analysis of carotenoid and tocopherol content in AyuFertin were conducted. The determination of estrus occurrence and natural mating were done by a fertile bull. The development of the large follicles (12.5–14.3 mm) in 85.7% of treated animals was observed on day 24 postpartum compared to 0% in controls. Clinical signs of estrus were recorded in 71.4% of the animals, followed by an 80% pregnancy rate versus 16% in controls within 70 days after calving. In conclusion, the supplementation of the bioactive herbal product AyuFertin from day 21 postpartum can stimulate the follicular growth in the buffalo’s ovary, which leads to the development of follicles with ovulatory capacity, followed by successful ovulation. The above-mentioned treatment resulted in a better pregnancy rate in the treated multiparous compared to the control buffaloes bred by natural service. |
| first_indexed | 2024-03-09T05:22:40Z |
| format | Article |
| id | doaj.art-e91f5c4159544f57b7806bbe78eb9b08 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T05:22:40Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-e91f5c4159544f57b7806bbe78eb9b082023-12-03T12:39:32ZengMDPI AGAnimals2076-26152021-02-0111242010.3390/ani11020420Resumption of Cyclic Ovarian Activity by Herbal Preparation AyuFertin in Bulgarian Murrah Buffaloes at Early PostpartumYordanka Ilieva0Nasko Vasilev1Ivan Fasulkov2Pencho Penchev3Desislava Abadjieva4Vanya Mladenova5Ayla Ilyazova6Dasha Mihaylova7Stanimir Yotov8Elena Kistanova9Agricultural Institute—Shumen, AA, 9700 Shumen, BulgariaDepartment Obstetrics, Reproduction and Reproductive Disorders, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, BulgariaDepartment Obstetrics, Reproduction and Reproductive Disorders, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, BulgariaAgricultural Institute—Shumen, AA, 9700 Shumen, BulgariaInstitute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaInstitute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaDepartment of Microbiology, University of Food Technologies, 4002 Plovdiv, BulgariaDepartment of Biotechnology, University of Food Technologies, 4002 Plovdiv, BulgariaDepartment Obstetrics, Reproduction and Reproductive Disorders, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, BulgariaInstitute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaThis study evaluates the phytochemical composition and ability of herbal preparation AyuFertin, supplemented shortly after calving, to resume the cyclic ovarian activity in postpartum Bulgarian Murrah buffaloes. In total, 13 healthy Bulgarian Murrah buffaloes at the age of 4–10 years, calved in March–April 2019 were involved in the experiment. Seven experimental buffaloes were treated individually per os with AyuFertin according to producer instruction. All animals were subjected to regular ultrasound examination of ovaries. GC-MS analysis of fatty acids composition and HPLC-DAD analysis of carotenoid and tocopherol content in AyuFertin were conducted. The determination of estrus occurrence and natural mating were done by a fertile bull. The development of the large follicles (12.5–14.3 mm) in 85.7% of treated animals was observed on day 24 postpartum compared to 0% in controls. Clinical signs of estrus were recorded in 71.4% of the animals, followed by an 80% pregnancy rate versus 16% in controls within 70 days after calving. In conclusion, the supplementation of the bioactive herbal product AyuFertin from day 21 postpartum can stimulate the follicular growth in the buffalo’s ovary, which leads to the development of follicles with ovulatory capacity, followed by successful ovulation. The above-mentioned treatment resulted in a better pregnancy rate in the treated multiparous compared to the control buffaloes bred by natural service.https://www.mdpi.com/2076-2615/11/2/420postpartum buffaloAyuFertin treatmentovarian activity |
| spellingShingle | Yordanka Ilieva Nasko Vasilev Ivan Fasulkov Pencho Penchev Desislava Abadjieva Vanya Mladenova Ayla Ilyazova Dasha Mihaylova Stanimir Yotov Elena Kistanova Resumption of Cyclic Ovarian Activity by Herbal Preparation AyuFertin in Bulgarian Murrah Buffaloes at Early Postpartum Animals postpartum buffalo AyuFertin treatment ovarian activity |
| title | Resumption of Cyclic Ovarian Activity by Herbal Preparation AyuFertin in Bulgarian Murrah Buffaloes at Early Postpartum |
| title_full | Resumption of Cyclic Ovarian Activity by Herbal Preparation AyuFertin in Bulgarian Murrah Buffaloes at Early Postpartum |
| title_fullStr | Resumption of Cyclic Ovarian Activity by Herbal Preparation AyuFertin in Bulgarian Murrah Buffaloes at Early Postpartum |
| title_full_unstemmed | Resumption of Cyclic Ovarian Activity by Herbal Preparation AyuFertin in Bulgarian Murrah Buffaloes at Early Postpartum |
| title_short | Resumption of Cyclic Ovarian Activity by Herbal Preparation AyuFertin in Bulgarian Murrah Buffaloes at Early Postpartum |
| title_sort | resumption of cyclic ovarian activity by herbal preparation ayufertin in bulgarian murrah buffaloes at early postpartum |
| topic | postpartum buffalo AyuFertin treatment ovarian activity |
| url | https://www.mdpi.com/2076-2615/11/2/420 |
| work_keys_str_mv | AT yordankailieva resumptionofcyclicovarianactivitybyherbalpreparationayufertininbulgarianmurrahbuffaloesatearlypostpartum AT naskovasilev resumptionofcyclicovarianactivitybyherbalpreparationayufertininbulgarianmurrahbuffaloesatearlypostpartum AT ivanfasulkov resumptionofcyclicovarianactivitybyherbalpreparationayufertininbulgarianmurrahbuffaloesatearlypostpartum AT penchopenchev resumptionofcyclicovarianactivitybyherbalpreparationayufertininbulgarianmurrahbuffaloesatearlypostpartum AT desislavaabadjieva resumptionofcyclicovarianactivitybyherbalpreparationayufertininbulgarianmurrahbuffaloesatearlypostpartum AT vanyamladenova resumptionofcyclicovarianactivitybyherbalpreparationayufertininbulgarianmurrahbuffaloesatearlypostpartum AT aylailyazova resumptionofcyclicovarianactivitybyherbalpreparationayufertininbulgarianmurrahbuffaloesatearlypostpartum AT dashamihaylova resumptionofcyclicovarianactivitybyherbalpreparationayufertininbulgarianmurrahbuffaloesatearlypostpartum AT stanimiryotov resumptionofcyclicovarianactivitybyherbalpreparationayufertininbulgarianmurrahbuffaloesatearlypostpartum AT elenakistanova resumptionofcyclicovarianactivitybyherbalpreparationayufertininbulgarianmurrahbuffaloesatearlypostpartum |