High-density linkage map construction in an autotetraploid blueberry population and detection of quantitative trait loci for anthocyanin content
Highbush blueberry (Vaccinium corymbosum, 2n = 4x = 48) is the most cultivated type of blueberry, both in New Zealand and overseas. Its perceived nutritional value is conferred by phytonutrients, particularly anthocyanins. Identifying the genetic mechanisms that control the biosynthesis of these met...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-09-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.965397/full |
| _version_ | 1818030230991798272 |
|---|---|
| author | Sara Montanari Susan Thomson Sarah Cordiner Catrin S. Günther Poppy Miller Cecilia H. Deng Tony McGhie Mareike Knäbel Toshi Foster Janice Turner David Chagné Richard Espley |
| author_facet | Sara Montanari Susan Thomson Sarah Cordiner Catrin S. Günther Poppy Miller Cecilia H. Deng Tony McGhie Mareike Knäbel Toshi Foster Janice Turner David Chagné Richard Espley |
| author_sort | Sara Montanari |
| collection | DOAJ |
| description | Highbush blueberry (Vaccinium corymbosum, 2n = 4x = 48) is the most cultivated type of blueberry, both in New Zealand and overseas. Its perceived nutritional value is conferred by phytonutrients, particularly anthocyanins. Identifying the genetic mechanisms that control the biosynthesis of these metabolites would enable faster development of cultivars with improved fruit qualities. Here, we used recently released tools for genetic mapping in autotetraploids to build a high-density linkage map in highbush blueberry and to detect quantitative trait loci (QTLs) for fruit anthocyanin content. Genotyping was performed by target sequencing, with ∼18,000 single nucleotide polymorphism (SNP) markers being mapped into 12 phased linkage groups (LGs). Fruits were harvested when ripe for two seasons and analyzed with high-performance liquid chromatography-mass spectrometry (HPLC-MS): 25 different anthocyanin compounds were identified and quantified. Two major QTLs that were stable across years were discovered, one on LG2 and one on LG4, and the underlying candidate genes were identified. Interestingly, the presence of anthocyanins containing acylated sugars appeared to be under strong genetic control. Information gained in this study will enable the design of molecular markers for marker-assisted selection and will help build a better understanding of the genetic control of anthocyanin biosynthesis in this crop. |
| first_indexed | 2024-12-10T05:32:17Z |
| format | Article |
| id | doaj.art-e91fc6466ecc412fa7bd7d1b4190ebda |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T05:32:17Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-e91fc6466ecc412fa7bd7d1b4190ebda2022-12-22T02:00:31ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.965397965397High-density linkage map construction in an autotetraploid blueberry population and detection of quantitative trait loci for anthocyanin contentSara Montanari0Susan Thomson1Sarah Cordiner2Catrin S. Günther3Poppy Miller4Cecilia H. Deng5Tony McGhie6Mareike Knäbel7Toshi Foster8Janice Turner9David Chagné10Richard Espley11The New Zealand Institute for Plant and Food Research Limited, Motueka, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Lincoln, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Palmerston North, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Ruakura, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Te Puke, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Auckland, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Palmerston North, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Palmerston North, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Motueka, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Motueka, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Palmerston North, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Auckland, New ZealandHighbush blueberry (Vaccinium corymbosum, 2n = 4x = 48) is the most cultivated type of blueberry, both in New Zealand and overseas. Its perceived nutritional value is conferred by phytonutrients, particularly anthocyanins. Identifying the genetic mechanisms that control the biosynthesis of these metabolites would enable faster development of cultivars with improved fruit qualities. Here, we used recently released tools for genetic mapping in autotetraploids to build a high-density linkage map in highbush blueberry and to detect quantitative trait loci (QTLs) for fruit anthocyanin content. Genotyping was performed by target sequencing, with ∼18,000 single nucleotide polymorphism (SNP) markers being mapped into 12 phased linkage groups (LGs). Fruits were harvested when ripe for two seasons and analyzed with high-performance liquid chromatography-mass spectrometry (HPLC-MS): 25 different anthocyanin compounds were identified and quantified. Two major QTLs that were stable across years were discovered, one on LG2 and one on LG4, and the underlying candidate genes were identified. Interestingly, the presence of anthocyanins containing acylated sugars appeared to be under strong genetic control. Information gained in this study will enable the design of molecular markers for marker-assisted selection and will help build a better understanding of the genetic control of anthocyanin biosynthesis in this crop.https://www.frontiersin.org/articles/10.3389/fpls.2022.965397/fullVaccinium corymbosumfruit qualityflavonoidsSNP markersgenetic mapcandidate genes |
| spellingShingle | Sara Montanari Susan Thomson Sarah Cordiner Catrin S. Günther Poppy Miller Cecilia H. Deng Tony McGhie Mareike Knäbel Toshi Foster Janice Turner David Chagné Richard Espley High-density linkage map construction in an autotetraploid blueberry population and detection of quantitative trait loci for anthocyanin content Frontiers in Plant Science Vaccinium corymbosum fruit quality flavonoids SNP markers genetic map candidate genes |
| title | High-density linkage map construction in an autotetraploid blueberry population and detection of quantitative trait loci for anthocyanin content |
| title_full | High-density linkage map construction in an autotetraploid blueberry population and detection of quantitative trait loci for anthocyanin content |
| title_fullStr | High-density linkage map construction in an autotetraploid blueberry population and detection of quantitative trait loci for anthocyanin content |
| title_full_unstemmed | High-density linkage map construction in an autotetraploid blueberry population and detection of quantitative trait loci for anthocyanin content |
| title_short | High-density linkage map construction in an autotetraploid blueberry population and detection of quantitative trait loci for anthocyanin content |
| title_sort | high density linkage map construction in an autotetraploid blueberry population and detection of quantitative trait loci for anthocyanin content |
| topic | Vaccinium corymbosum fruit quality flavonoids SNP markers genetic map candidate genes |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.965397/full |
| work_keys_str_mv | AT saramontanari highdensitylinkagemapconstructioninanautotetraploidblueberrypopulationanddetectionofquantitativetraitlociforanthocyanincontent AT susanthomson highdensitylinkagemapconstructioninanautotetraploidblueberrypopulationanddetectionofquantitativetraitlociforanthocyanincontent AT sarahcordiner highdensitylinkagemapconstructioninanautotetraploidblueberrypopulationanddetectionofquantitativetraitlociforanthocyanincontent AT catrinsgunther highdensitylinkagemapconstructioninanautotetraploidblueberrypopulationanddetectionofquantitativetraitlociforanthocyanincontent AT poppymiller highdensitylinkagemapconstructioninanautotetraploidblueberrypopulationanddetectionofquantitativetraitlociforanthocyanincontent AT ceciliahdeng highdensitylinkagemapconstructioninanautotetraploidblueberrypopulationanddetectionofquantitativetraitlociforanthocyanincontent AT tonymcghie highdensitylinkagemapconstructioninanautotetraploidblueberrypopulationanddetectionofquantitativetraitlociforanthocyanincontent AT mareikeknabel highdensitylinkagemapconstructioninanautotetraploidblueberrypopulationanddetectionofquantitativetraitlociforanthocyanincontent AT toshifoster highdensitylinkagemapconstructioninanautotetraploidblueberrypopulationanddetectionofquantitativetraitlociforanthocyanincontent AT janiceturner highdensitylinkagemapconstructioninanautotetraploidblueberrypopulationanddetectionofquantitativetraitlociforanthocyanincontent AT davidchagne highdensitylinkagemapconstructioninanautotetraploidblueberrypopulationanddetectionofquantitativetraitlociforanthocyanincontent AT richardespley highdensitylinkagemapconstructioninanautotetraploidblueberrypopulationanddetectionofquantitativetraitlociforanthocyanincontent |