Optimization and Characterization of Biosurfactant Production from Marine Vibrio sp. strain 3B-2
A biosurfactant-producing bacterium, designated 3B-2, was isolated from marine sediment and identified as Vibrio sp. by 16S rRNA gene sequencing. The culture medium composition was optimized to increase the capability of 3B-2 for producing biosurfactant. The produced biosurfactant was characterized...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-09-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00976/full |
| _version_ | 1818973523118391296 |
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| author | Xiaoke eHu Caixia eWang Peng eWang |
| author_facet | Xiaoke eHu Caixia eWang Peng eWang |
| author_sort | Xiaoke eHu |
| collection | DOAJ |
| description | A biosurfactant-producing bacterium, designated 3B-2, was isolated from marine sediment and identified as Vibrio sp. by 16S rRNA gene sequencing. The culture medium composition was optimized to increase the capability of 3B-2 for producing biosurfactant. The produced biosurfactant was characterized in terms of protein concentration, surface tension and oil-displacement efficiency. The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride and 0.1% disodium hydrogen phosphate. Under optimal conditions (28°C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm. Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation. |
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| format | Article |
| id | doaj.art-e921baa730854f23a2e044da97c59257 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-20T15:25:31Z |
| publishDate | 2015-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-e921baa730854f23a2e044da97c592572022-12-21T19:35:52ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-09-01610.3389/fmicb.2015.00976156592Optimization and Characterization of Biosurfactant Production from Marine Vibrio sp. strain 3B-2Xiaoke eHu0Caixia eWang1Peng eWang2Yantai Institute of Coastal Zone Research, Chinese Academy of SciencesYantai Institute of Coastal Zone Research, Chinese Academy of Sciences,University of Chinese Academy of SciencesOcean University of ChinaA biosurfactant-producing bacterium, designated 3B-2, was isolated from marine sediment and identified as Vibrio sp. by 16S rRNA gene sequencing. The culture medium composition was optimized to increase the capability of 3B-2 for producing biosurfactant. The produced biosurfactant was characterized in terms of protein concentration, surface tension and oil-displacement efficiency. The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride and 0.1% disodium hydrogen phosphate. Under optimal conditions (28°C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm. Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00976/fullGlycoproteinsSurface TensionVibrio alginolyticusoptimal productionmicrobial biosurfactant |
| spellingShingle | Xiaoke eHu Caixia eWang Peng eWang Optimization and Characterization of Biosurfactant Production from Marine Vibrio sp. strain 3B-2 Frontiers in Microbiology Glycoproteins Surface Tension Vibrio alginolyticus optimal production microbial biosurfactant |
| title | Optimization and Characterization of Biosurfactant Production from Marine Vibrio sp. strain 3B-2 |
| title_full | Optimization and Characterization of Biosurfactant Production from Marine Vibrio sp. strain 3B-2 |
| title_fullStr | Optimization and Characterization of Biosurfactant Production from Marine Vibrio sp. strain 3B-2 |
| title_full_unstemmed | Optimization and Characterization of Biosurfactant Production from Marine Vibrio sp. strain 3B-2 |
| title_short | Optimization and Characterization of Biosurfactant Production from Marine Vibrio sp. strain 3B-2 |
| title_sort | optimization and characterization of biosurfactant production from marine vibrio sp strain 3b 2 |
| topic | Glycoproteins Surface Tension Vibrio alginolyticus optimal production microbial biosurfactant |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00976/full |
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