Cortical dynein pulling mechanism is regulated by differentially targeted attachment molecule Num1
Cortical dynein generates pulling forces via microtubule (MT) end capture-shrinkage and lateral MT sliding mechanisms. In Saccharomyces cerevisiae, the dynein attachment molecule Num1 interacts with endoplasmic reticulum (ER) and mitochondria to facilitate spindle positioning across the mother-bud n...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-08-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/36745 |
| _version_ | 1811202737860247552 |
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| author | Safia Omer Samuel R Greenberg Wei-Lih Lee |
| author_facet | Safia Omer Samuel R Greenberg Wei-Lih Lee |
| author_sort | Safia Omer |
| collection | DOAJ |
| description | Cortical dynein generates pulling forces via microtubule (MT) end capture-shrinkage and lateral MT sliding mechanisms. In Saccharomyces cerevisiae, the dynein attachment molecule Num1 interacts with endoplasmic reticulum (ER) and mitochondria to facilitate spindle positioning across the mother-bud neck, but direct evidence for how these cortical contacts regulate dynein-dependent pulling forces is lacking. We show that loss of Scs2/Scs22, ER tethering proteins, resulted in defective Num1 distribution and loss of dynein-dependent MT sliding, the hallmark of dynein function. Cells lacking Scs2/Scs22 performed spindle positioning via MT end capture-shrinkage mechanism, requiring dynein anchorage to an ER- and mitochondria-independent population of Num1, dynein motor activity, and CAP-Gly domain of dynactin Nip100/p150Glued subunit. Additionally, a CAAX-targeted Num1 rescued loss of lateral patches and MT sliding in the absence of Scs2/Scs22. These results reveal distinct populations of Num1 and underline the importance of their spatial distribution as a critical factor for regulating dynein pulling force. |
| first_indexed | 2024-04-12T02:43:41Z |
| format | Article |
| id | doaj.art-e924560c68244b31a7a1c6f464c18fd7 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:43:41Z |
| publishDate | 2018-08-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-e924560c68244b31a7a1c6f464c18fd72022-12-22T03:51:15ZengeLife Sciences Publications LtdeLife2050-084X2018-08-01710.7554/eLife.36745Cortical dynein pulling mechanism is regulated by differentially targeted attachment molecule Num1Safia Omer0https://orcid.org/0000-0002-3009-4496Samuel R Greenberg1https://orcid.org/0000-0002-4176-3958Wei-Lih Lee2https://orcid.org/0000-0002-5606-4754Molecular and Cellular Biology Graduate Program, University of Massachusetts, Amherst, United StatesDepartment of Biological Sciences, Dartmouth College, Hanover, United StatesDepartment of Biological Sciences, Dartmouth College, Hanover, United StatesCortical dynein generates pulling forces via microtubule (MT) end capture-shrinkage and lateral MT sliding mechanisms. In Saccharomyces cerevisiae, the dynein attachment molecule Num1 interacts with endoplasmic reticulum (ER) and mitochondria to facilitate spindle positioning across the mother-bud neck, but direct evidence for how these cortical contacts regulate dynein-dependent pulling forces is lacking. We show that loss of Scs2/Scs22, ER tethering proteins, resulted in defective Num1 distribution and loss of dynein-dependent MT sliding, the hallmark of dynein function. Cells lacking Scs2/Scs22 performed spindle positioning via MT end capture-shrinkage mechanism, requiring dynein anchorage to an ER- and mitochondria-independent population of Num1, dynein motor activity, and CAP-Gly domain of dynactin Nip100/p150Glued subunit. Additionally, a CAAX-targeted Num1 rescued loss of lateral patches and MT sliding in the absence of Scs2/Scs22. These results reveal distinct populations of Num1 and underline the importance of their spatial distribution as a critical factor for regulating dynein pulling force.https://elifesciences.org/articles/36745nuclear migrationspindle positioningdyneinyeastdynactin |
| spellingShingle | Safia Omer Samuel R Greenberg Wei-Lih Lee Cortical dynein pulling mechanism is regulated by differentially targeted attachment molecule Num1 eLife nuclear migration spindle positioning dynein yeast dynactin |
| title | Cortical dynein pulling mechanism is regulated by differentially targeted attachment molecule Num1 |
| title_full | Cortical dynein pulling mechanism is regulated by differentially targeted attachment molecule Num1 |
| title_fullStr | Cortical dynein pulling mechanism is regulated by differentially targeted attachment molecule Num1 |
| title_full_unstemmed | Cortical dynein pulling mechanism is regulated by differentially targeted attachment molecule Num1 |
| title_short | Cortical dynein pulling mechanism is regulated by differentially targeted attachment molecule Num1 |
| title_sort | cortical dynein pulling mechanism is regulated by differentially targeted attachment molecule num1 |
| topic | nuclear migration spindle positioning dynein yeast dynactin |
| url | https://elifesciences.org/articles/36745 |
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