Genome-wide analysis reveals regulatory mechanisms and expression patterns of TGA genes in peanut under abiotic stress and hormone treatments
IntroductionThe TGA transcription factors, plays a crucial role in regulating gene expression. In cultivated peanut (Arachis hypogaea), which faces abiotic stress challenges, understanding the role of TGAs is important.MethodsIn this study, we conducted a comprehensive in analysis of the TGA gene fa...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1269200/full |
| _version_ | 1797491041230651392 |
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| author | Chao Zhong Yu Liu Zhao Li Xiaoguang Wang Chunji Jiang Xinhua Zhao Shuli Kang Xibo Liu Shuli Zhao Jing Wang He Zhang Yuning Huang Yuning Huang Haiqiu Yu Renfeng Xue Renfeng Xue |
| author_facet | Chao Zhong Yu Liu Zhao Li Xiaoguang Wang Chunji Jiang Xinhua Zhao Shuli Kang Xibo Liu Shuli Zhao Jing Wang He Zhang Yuning Huang Yuning Huang Haiqiu Yu Renfeng Xue Renfeng Xue |
| author_sort | Chao Zhong |
| collection | DOAJ |
| description | IntroductionThe TGA transcription factors, plays a crucial role in regulating gene expression. In cultivated peanut (Arachis hypogaea), which faces abiotic stress challenges, understanding the role of TGAs is important.MethodsIn this study, we conducted a comprehensive in analysis of the TGA gene family in peanut to elucidate their regulatory mechanisms and expression patterns under abiotic stress and hormone treatments. Furthermore, functional studies on the representative AhTGA gene in peanut cultivars were conducted using transgenic Arabidopsis and soybean hair roots.ResultsThe genome-wide analysis revealed that a total of 20 AhTGA genes were identified and classified into five subfamilies. Collinearity analysis revealed that AhTGA genes lack tandem duplication, and their amplification in the cultivated peanut genome primarily relies on the whole-genome duplication of the diploid wild peanut to form tetraploid cultivated peanut, as well as segment duplication between the A and B subgenomes. Promoter and Protein-protein interaction analysis identified a wide range of cis-acting elements and potential interacting proteins associated with growth and development, hormones, and stress responses. Expression patterns of AhTGA genes in different tissues, under abiotic stress conditions for low temperature and drought, and in response to hormonal stimuli revealed that seven AhTGA genes from groups I (AhTGA04, AhTGA14 and AhTGA20) and II (AhTGA07, AhTGA11, AhTGA16 and AhTGA18) are involved in the response to abiotic stress and hormonal stimuli. The hormone treatment results indicate that these AhTGA genes primarily respond to the regulation of jasmonic acid and salicylic acid. Overexpressing AhTGA11 in Arabidopsis enhances resistance to cold and drought stress by increasing antioxidant activities and altering endogenous hormone levels, particularly ABA, SA and JA.DiscussionThe AhTGA genes plays a crucial role in hormone regulation and stress response during peanut growth and development. The findings provide insights into peanut's abiotic stress tolerance mechanisms and pave the way for future functional studies. |
| first_indexed | 2024-03-10T00:41:44Z |
| format | Article |
| id | doaj.art-e92b68edc6b2455daca00aeea403b7b1 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-10T00:41:44Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-e92b68edc6b2455daca00aeea403b7b12023-11-23T15:06:42ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-11-011410.3389/fpls.2023.12692001269200Genome-wide analysis reveals regulatory mechanisms and expression patterns of TGA genes in peanut under abiotic stress and hormone treatmentsChao Zhong0Yu Liu1Zhao Li2Xiaoguang Wang3Chunji Jiang4Xinhua Zhao5Shuli Kang6Xibo Liu7Shuli Zhao8Jing Wang9He Zhang10Yuning Huang11Yuning Huang12Haiqiu Yu13Renfeng Xue14Renfeng Xue15College of Agronomy, Shenyang Agricultural University, Shenyang, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, ChinaCrop Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang, ChinaLiaoning Provincial Key Laboratory of Miscellaneous Grain Germplasm Innovation and Genetic Breeding, Liaoning Academy of Agricultural Sciences, Shenyang, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, ChinaCrop Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang, ChinaLiaoning Provincial Key Laboratory of Miscellaneous Grain Germplasm Innovation and Genetic Breeding, Liaoning Academy of Agricultural Sciences, Shenyang, ChinaIntroductionThe TGA transcription factors, plays a crucial role in regulating gene expression. In cultivated peanut (Arachis hypogaea), which faces abiotic stress challenges, understanding the role of TGAs is important.MethodsIn this study, we conducted a comprehensive in analysis of the TGA gene family in peanut to elucidate their regulatory mechanisms and expression patterns under abiotic stress and hormone treatments. Furthermore, functional studies on the representative AhTGA gene in peanut cultivars were conducted using transgenic Arabidopsis and soybean hair roots.ResultsThe genome-wide analysis revealed that a total of 20 AhTGA genes were identified and classified into five subfamilies. Collinearity analysis revealed that AhTGA genes lack tandem duplication, and their amplification in the cultivated peanut genome primarily relies on the whole-genome duplication of the diploid wild peanut to form tetraploid cultivated peanut, as well as segment duplication between the A and B subgenomes. Promoter and Protein-protein interaction analysis identified a wide range of cis-acting elements and potential interacting proteins associated with growth and development, hormones, and stress responses. Expression patterns of AhTGA genes in different tissues, under abiotic stress conditions for low temperature and drought, and in response to hormonal stimuli revealed that seven AhTGA genes from groups I (AhTGA04, AhTGA14 and AhTGA20) and II (AhTGA07, AhTGA11, AhTGA16 and AhTGA18) are involved in the response to abiotic stress and hormonal stimuli. The hormone treatment results indicate that these AhTGA genes primarily respond to the regulation of jasmonic acid and salicylic acid. Overexpressing AhTGA11 in Arabidopsis enhances resistance to cold and drought stress by increasing antioxidant activities and altering endogenous hormone levels, particularly ABA, SA and JA.DiscussionThe AhTGA genes plays a crucial role in hormone regulation and stress response during peanut growth and development. The findings provide insights into peanut's abiotic stress tolerance mechanisms and pave the way for future functional studies.https://www.frontiersin.org/articles/10.3389/fpls.2023.1269200/fullTGA genespeanut (Arachis hypogaea)abiotic stresshormone signalingtranscription factors |
| spellingShingle | Chao Zhong Yu Liu Zhao Li Xiaoguang Wang Chunji Jiang Xinhua Zhao Shuli Kang Xibo Liu Shuli Zhao Jing Wang He Zhang Yuning Huang Yuning Huang Haiqiu Yu Renfeng Xue Renfeng Xue Genome-wide analysis reveals regulatory mechanisms and expression patterns of TGA genes in peanut under abiotic stress and hormone treatments Frontiers in Plant Science TGA genes peanut (Arachis hypogaea) abiotic stress hormone signaling transcription factors |
| title | Genome-wide analysis reveals regulatory mechanisms and expression patterns of TGA genes in peanut under abiotic stress and hormone treatments |
| title_full | Genome-wide analysis reveals regulatory mechanisms and expression patterns of TGA genes in peanut under abiotic stress and hormone treatments |
| title_fullStr | Genome-wide analysis reveals regulatory mechanisms and expression patterns of TGA genes in peanut under abiotic stress and hormone treatments |
| title_full_unstemmed | Genome-wide analysis reveals regulatory mechanisms and expression patterns of TGA genes in peanut under abiotic stress and hormone treatments |
| title_short | Genome-wide analysis reveals regulatory mechanisms and expression patterns of TGA genes in peanut under abiotic stress and hormone treatments |
| title_sort | genome wide analysis reveals regulatory mechanisms and expression patterns of tga genes in peanut under abiotic stress and hormone treatments |
| topic | TGA genes peanut (Arachis hypogaea) abiotic stress hormone signaling transcription factors |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1269200/full |
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