Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran
Background: The aim of this study was to apply the nested-PCR and bioassay methods in detection and genotyping of Toxoplasma gondii infection in provided sheep aborted fetus samples from Qazvin Province of Iran. Methods: Eighteen sheep aborted fetal samples were studied by nested-PCR-RFLP, histopath...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2012-09-01
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| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/254 |
| _version_ | 1819280581497716736 |
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| author | GR Habibi AR Imani MR Gholami MH Hablolvarid AM Behroozikhah M Lotfi M Kamalzade E Najjar K Esmaeil-Nia S Bozorgi |
| author_facet | GR Habibi AR Imani MR Gholami MH Hablolvarid AM Behroozikhah M Lotfi M Kamalzade E Najjar K Esmaeil-Nia S Bozorgi |
| author_sort | GR Habibi |
| collection | DOAJ |
| description | Background: The aim of this study was to apply the nested-PCR and bioassay methods in detection and genotyping of Toxoplasma gondii infection in provided sheep aborted fetus samples from Qazvin Province of Iran.
Methods: Eighteen sheep aborted fetal samples were studied by nested-PCR-RFLP, histopathological observation and microbiological assay. Bioassay in mice was carried out by inoculating the brain samples intraperitoneally.
Results: The results demonstrated the frequency of 66% infected sheep aborted fetal samples with T. gondii type one. Although we could not isolate any parasite from inoculated mice even after three passages, but it was confirmed histopathologically formation of cyst like bodies in prepared mice brain sections.
Conclusion: The results of the performed nested-PCR and formation of brain cyst in inoculated mice exhibited that T. gondii type one infection might be considered as one of the major causative agents for abortion in ewes. |
| first_indexed | 2024-12-24T00:46:04Z |
| format | Article |
| id | doaj.art-e92d166f66724cc8b0dbdad710d0dd63 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-24T00:46:04Z |
| publishDate | 2012-09-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-e92d166f66724cc8b0dbdad710d0dd632022-12-21T17:23:49ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2012-09-0173Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of IranGR Habibi0AR Imani1MR Gholami2MH Hablolvarid3AM Behroozikhah4M Lotfi5M Kamalzade6E Najjar7K Esmaeil-Nia8S Bozorgi9Parasite Vaccine Research and Production Department, Razi Vaccine and Serum Research Institute, Karaj, IranVeterinary College, Islamic Azad University, Karaj, IranPathology Department, Razi Vaccine and Serum Research Institute, Karaj, IranBrucellosis Vaccine Research and Production Department, Razi Vaccine and Serum Research Institute, Karaj, IranBrucellosis Vaccine Research and Production Department, Razi Vaccine and Serum Research Institute, Karaj, IranQuality Control of Biological Products group, Razi Vaccine and Serum Research Institute, Karaj, IranQuality Control of Biological Products group, Razi Vaccine and Serum Research Institute, Karaj, IranVeterinary Organization of Qazvin Province, Qazvin, IranParasite Vaccine Research and Production Department, Razi Vaccine and Serum Research Institute, Karaj, IranParasite Vaccine Research and Production Department, Razi Vaccine and Serum Research Institute, Karaj, IranBackground: The aim of this study was to apply the nested-PCR and bioassay methods in detection and genotyping of Toxoplasma gondii infection in provided sheep aborted fetus samples from Qazvin Province of Iran. Methods: Eighteen sheep aborted fetal samples were studied by nested-PCR-RFLP, histopathological observation and microbiological assay. Bioassay in mice was carried out by inoculating the brain samples intraperitoneally. Results: The results demonstrated the frequency of 66% infected sheep aborted fetal samples with T. gondii type one. Although we could not isolate any parasite from inoculated mice even after three passages, but it was confirmed histopathologically formation of cyst like bodies in prepared mice brain sections. Conclusion: The results of the performed nested-PCR and formation of brain cyst in inoculated mice exhibited that T. gondii type one infection might be considered as one of the major causative agents for abortion in ewes.https://ijpa.tums.ac.ir/index.php/ijpa/article/view/254Toxoplasma gondiiSheep abortionFetusPCRBioassayGenotyping |
| spellingShingle | GR Habibi AR Imani MR Gholami MH Hablolvarid AM Behroozikhah M Lotfi M Kamalzade E Najjar K Esmaeil-Nia S Bozorgi Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran Iranian Journal of Parasitology Toxoplasma gondii Sheep abortion Fetus PCR Bioassay Genotyping |
| title | Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran |
| title_full | Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran |
| title_fullStr | Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran |
| title_full_unstemmed | Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran |
| title_short | Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran |
| title_sort | detection and identification of toxoplasma gondii type one infection in sheep aborted fetuses in qazvin province of iran |
| topic | Toxoplasma gondii Sheep abortion Fetus PCR Bioassay Genotyping |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/254 |
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