Human tissue-resident peritoneal macrophages reveal resistance towards oxidative cell stress induced by non-invasive physical plasma
In the context of multimodal treatments for abdominal cancer, including procedures such as cytoreductive surgery and intraperitoneal chemotherapy, recurrence rates remain high, and long-term survival benefits are uncertain due to post-operative complications. Notably, treatment-limiting side effects...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1357340/full |
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| author | Laura Schultze-Rhonhof Julia Marzi Julia Marzi Daniel Alejandro Carvajal Berrio Myriam Holl Theresa Braun Theresa Braun Felix Schäfer-Ruoff Jürgen Andress Cornelia Bachmann Markus Templin Sara Y. Brucker Katja Schenke-Layland Katja Schenke-Layland Martin Weiss Martin Weiss |
| author_facet | Laura Schultze-Rhonhof Julia Marzi Julia Marzi Daniel Alejandro Carvajal Berrio Myriam Holl Theresa Braun Theresa Braun Felix Schäfer-Ruoff Jürgen Andress Cornelia Bachmann Markus Templin Sara Y. Brucker Katja Schenke-Layland Katja Schenke-Layland Martin Weiss Martin Weiss |
| author_sort | Laura Schultze-Rhonhof |
| collection | DOAJ |
| description | In the context of multimodal treatments for abdominal cancer, including procedures such as cytoreductive surgery and intraperitoneal chemotherapy, recurrence rates remain high, and long-term survival benefits are uncertain due to post-operative complications. Notably, treatment-limiting side effects often arise from an uncontrolled activation of the immune system, particularly peritoneally localized macrophages, leading to massive cytokine secretion and phenotype changes. Exploring alternatives, an increasing number of studies investigated the potential of plasma-activated liquids (PAL) for adjuvant peritoneal cancer treatment, aiming to mitigate side effects, preserve healthy tissue, and reduce cytotoxicity towards non-cancer cells. To assess the non-toxicity of PAL, we isolated primary human macrophages from the peritoneum and subjected them to PAL exposure. Employing an extensive methodological spectrum, including flow cytometry, Raman microspectroscopy, and DigiWest protein analysis, we observed a pronounced resistance of macrophages towards PAL. This resistance was characterized by an upregulation of proliferation and anti-oxidative pathways, countering PAL-derived oxidative stress-induced cell death. The observed cellular effects of PAL treatment on human tissue-resident peritoneal macrophages unveil a potential avenue for PAL-derived immunomodulatory effects within the human peritoneal cavity. Our findings contribute to understanding the intricate interplay between PAL and macrophages, shedding light on the promising prospects for PAL in the adjuvant treatment of peritoneal cancer. |
| first_indexed | 2024-03-07T15:34:11Z |
| format | Article |
| id | doaj.art-e92fadf743054b5e8b5e82f7dcd97a82 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-07T15:34:11Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-e92fadf743054b5e8b5e82f7dcd97a822024-03-05T13:50:46ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-03-011510.3389/fimmu.2024.13573401357340Human tissue-resident peritoneal macrophages reveal resistance towards oxidative cell stress induced by non-invasive physical plasmaLaura Schultze-Rhonhof0Julia Marzi1Julia Marzi2Daniel Alejandro Carvajal Berrio3Myriam Holl4Theresa Braun5Theresa Braun6Felix Schäfer-Ruoff7Jürgen Andress8Cornelia Bachmann9Markus Templin10Sara Y. Brucker11Katja Schenke-Layland12Katja Schenke-Layland13Martin Weiss14Martin Weiss15Department of Women’s Health Tübingen, University of Tübingen, Tübingen, GermanyInstitute of Biomedical Engineering, Department for Medical Technologies and Regenerative Medicine, University of Tübingen, Tübingen, GermanyNatural and Medical Sciences Institute (NMI) at the University of Tübingen, Reutlingen, GermanyInstitute of Biomedical Engineering, Department for Medical Technologies and Regenerative Medicine, University of Tübingen, Tübingen, GermanyDepartment of Women’s Health Tübingen, University of Tübingen, Tübingen, GermanyNatural and Medical Sciences Institute (NMI) at the University of Tübingen, Reutlingen, GermanyUniversity Development, Research and Transfer, University of Konstanz, Konstanz, GermanyNatural and Medical Sciences Institute (NMI) at the University of Tübingen, Reutlingen, GermanyDepartment of Women’s Health Tübingen, University of Tübingen, Tübingen, GermanyDepartment of Women’s Health Tübingen, University of Tübingen, Tübingen, GermanyNatural and Medical Sciences Institute (NMI) at the University of Tübingen, Reutlingen, GermanyDepartment of Women’s Health Tübingen, University of Tübingen, Tübingen, GermanyInstitute of Biomedical Engineering, Department for Medical Technologies and Regenerative Medicine, University of Tübingen, Tübingen, GermanyNatural and Medical Sciences Institute (NMI) at the University of Tübingen, Reutlingen, GermanyDepartment of Women’s Health Tübingen, University of Tübingen, Tübingen, GermanyNatural and Medical Sciences Institute (NMI) at the University of Tübingen, Reutlingen, GermanyIn the context of multimodal treatments for abdominal cancer, including procedures such as cytoreductive surgery and intraperitoneal chemotherapy, recurrence rates remain high, and long-term survival benefits are uncertain due to post-operative complications. Notably, treatment-limiting side effects often arise from an uncontrolled activation of the immune system, particularly peritoneally localized macrophages, leading to massive cytokine secretion and phenotype changes. Exploring alternatives, an increasing number of studies investigated the potential of plasma-activated liquids (PAL) for adjuvant peritoneal cancer treatment, aiming to mitigate side effects, preserve healthy tissue, and reduce cytotoxicity towards non-cancer cells. To assess the non-toxicity of PAL, we isolated primary human macrophages from the peritoneum and subjected them to PAL exposure. Employing an extensive methodological spectrum, including flow cytometry, Raman microspectroscopy, and DigiWest protein analysis, we observed a pronounced resistance of macrophages towards PAL. This resistance was characterized by an upregulation of proliferation and anti-oxidative pathways, countering PAL-derived oxidative stress-induced cell death. The observed cellular effects of PAL treatment on human tissue-resident peritoneal macrophages unveil a potential avenue for PAL-derived immunomodulatory effects within the human peritoneal cavity. Our findings contribute to understanding the intricate interplay between PAL and macrophages, shedding light on the promising prospects for PAL in the adjuvant treatment of peritoneal cancer.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1357340/fullnon-invasive physical plasma (NIPP)cold atmospheric plasma (CAP)plasma-activated media (PAM)plasma-treated solutions (PTS)human primary macrophagesimmune response |
| spellingShingle | Laura Schultze-Rhonhof Julia Marzi Julia Marzi Daniel Alejandro Carvajal Berrio Myriam Holl Theresa Braun Theresa Braun Felix Schäfer-Ruoff Jürgen Andress Cornelia Bachmann Markus Templin Sara Y. Brucker Katja Schenke-Layland Katja Schenke-Layland Martin Weiss Martin Weiss Human tissue-resident peritoneal macrophages reveal resistance towards oxidative cell stress induced by non-invasive physical plasma Frontiers in Immunology non-invasive physical plasma (NIPP) cold atmospheric plasma (CAP) plasma-activated media (PAM) plasma-treated solutions (PTS) human primary macrophages immune response |
| title | Human tissue-resident peritoneal macrophages reveal resistance towards oxidative cell stress induced by non-invasive physical plasma |
| title_full | Human tissue-resident peritoneal macrophages reveal resistance towards oxidative cell stress induced by non-invasive physical plasma |
| title_fullStr | Human tissue-resident peritoneal macrophages reveal resistance towards oxidative cell stress induced by non-invasive physical plasma |
| title_full_unstemmed | Human tissue-resident peritoneal macrophages reveal resistance towards oxidative cell stress induced by non-invasive physical plasma |
| title_short | Human tissue-resident peritoneal macrophages reveal resistance towards oxidative cell stress induced by non-invasive physical plasma |
| title_sort | human tissue resident peritoneal macrophages reveal resistance towards oxidative cell stress induced by non invasive physical plasma |
| topic | non-invasive physical plasma (NIPP) cold atmospheric plasma (CAP) plasma-activated media (PAM) plasma-treated solutions (PTS) human primary macrophages immune response |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1357340/full |
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