Bisphenol analogues induced metabolic effects through eliciting intestinal cell heterogeneous response
The metabolic effects of endocrine-disrupting chemicals, such as bisphenol analogues, have drawn increasing attention. Bisphenol A (BPA) usage is associated with the occurrence of many metabolic diseases. With the restricted use of BPA, alternatives like bisphenol F (BPF) and bisphenol AF (BPAF) hav...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | Environment International |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0160412022002148 |
| _version_ | 1828317920423313408 |
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| author | Xiyan Mu Suzhen Qi Hui Wang Lilai Yuan Chengju Wang Yingren Li Jing Qiu |
| author_facet | Xiyan Mu Suzhen Qi Hui Wang Lilai Yuan Chengju Wang Yingren Li Jing Qiu |
| author_sort | Xiyan Mu |
| collection | DOAJ |
| description | The metabolic effects of endocrine-disrupting chemicals, such as bisphenol analogues, have drawn increasing attention. Bisphenol A (BPA) usage is associated with the occurrence of many metabolic diseases. With the restricted use of BPA, alternatives like bisphenol F (BPF) and bisphenol AF (BPAF) have been greatly introduced for industrial manufacture, and brings new hazard to public health. To understand how bisphenol analogues induced metabolic effects, zebrafish are continuous exposed to environmental level (0.5 μg/L) of BPA, BPF and BPAF since embryonic stage, and identified hepatic steatosis and insulin resistance at 60-day post fertilization. Hepatic transcriptional profile indicated that pancreatic disease pathways were activated by BPA, but were inhibited by BPF. At the same time, increased lipid secretion and gluconeogenesis pathways in zebrafish liver was found post BPAF exposure. Significant inflammatory response, histological injury and increased mucus secretion was detected in zebrafish intestine post exposure of three bisphenol analogues. Single-cell RNA sequencing of zebrafish intestinal cells revealed activation of lipid uptake and absorption pathways in enterocyte lineages, which well explained the hepatic steatosis induced by BPA and BPF. Besides, genes related to carbohydrate metabolism, diabetes and insulin resistance were activated in intestinal immune cell types by three bisphenol analogues. These findings indicated that BPA and its alternatives could lead to abnormal lipid and carbohydrate metabolism of zebrafish through inducing cell heterogeneous changes in gut, and revealed both molecular and cellular mechanism in mediating this effect. |
| first_indexed | 2024-04-13T17:35:33Z |
| format | Article |
| id | doaj.art-e930d8fb0ac845da9150f001e95fae24 |
| institution | Directory Open Access Journal |
| issn | 0160-4120 |
| language | English |
| last_indexed | 2024-04-13T17:35:33Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environment International |
| spelling | doaj.art-e930d8fb0ac845da9150f001e95fae242022-12-22T02:37:25ZengElsevierEnvironment International0160-41202022-07-01165107287Bisphenol analogues induced metabolic effects through eliciting intestinal cell heterogeneous responseXiyan Mu0Suzhen Qi1Hui Wang2Lilai Yuan3Chengju Wang4Yingren Li5Jing Qiu6Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China; Fishery Resource and Environment Research Center, Chinese Academy of Fishery Sciences, Beijing, People’s Republic of China; Corresponding authors.Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of ChinaFishery Resource and Environment Research Center, Chinese Academy of Fishery Sciences, Beijing, People’s Republic of ChinaFishery Resource and Environment Research Center, Chinese Academy of Fishery Sciences, Beijing, People’s Republic of ChinaCollege of Sciences, China Agricultural University, Beijing, People’s Republic of ChinaFishery Resource and Environment Research Center, Chinese Academy of Fishery Sciences, Beijing, People’s Republic of ChinaInstitute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China; Corresponding authors.The metabolic effects of endocrine-disrupting chemicals, such as bisphenol analogues, have drawn increasing attention. Bisphenol A (BPA) usage is associated with the occurrence of many metabolic diseases. With the restricted use of BPA, alternatives like bisphenol F (BPF) and bisphenol AF (BPAF) have been greatly introduced for industrial manufacture, and brings new hazard to public health. To understand how bisphenol analogues induced metabolic effects, zebrafish are continuous exposed to environmental level (0.5 μg/L) of BPA, BPF and BPAF since embryonic stage, and identified hepatic steatosis and insulin resistance at 60-day post fertilization. Hepatic transcriptional profile indicated that pancreatic disease pathways were activated by BPA, but were inhibited by BPF. At the same time, increased lipid secretion and gluconeogenesis pathways in zebrafish liver was found post BPAF exposure. Significant inflammatory response, histological injury and increased mucus secretion was detected in zebrafish intestine post exposure of three bisphenol analogues. Single-cell RNA sequencing of zebrafish intestinal cells revealed activation of lipid uptake and absorption pathways in enterocyte lineages, which well explained the hepatic steatosis induced by BPA and BPF. Besides, genes related to carbohydrate metabolism, diabetes and insulin resistance were activated in intestinal immune cell types by three bisphenol analogues. These findings indicated that BPA and its alternatives could lead to abnormal lipid and carbohydrate metabolism of zebrafish through inducing cell heterogeneous changes in gut, and revealed both molecular and cellular mechanism in mediating this effect.http://www.sciencedirect.com/science/article/pii/S0160412022002148Bisphenol analoguesIntestinal cell heterogeneityMetabolic effectHepatic steatosisInsulin resistanceZebrafish |
| spellingShingle | Xiyan Mu Suzhen Qi Hui Wang Lilai Yuan Chengju Wang Yingren Li Jing Qiu Bisphenol analogues induced metabolic effects through eliciting intestinal cell heterogeneous response Environment International Bisphenol analogues Intestinal cell heterogeneity Metabolic effect Hepatic steatosis Insulin resistance Zebrafish |
| title | Bisphenol analogues induced metabolic effects through eliciting intestinal cell heterogeneous response |
| title_full | Bisphenol analogues induced metabolic effects through eliciting intestinal cell heterogeneous response |
| title_fullStr | Bisphenol analogues induced metabolic effects through eliciting intestinal cell heterogeneous response |
| title_full_unstemmed | Bisphenol analogues induced metabolic effects through eliciting intestinal cell heterogeneous response |
| title_short | Bisphenol analogues induced metabolic effects through eliciting intestinal cell heterogeneous response |
| title_sort | bisphenol analogues induced metabolic effects through eliciting intestinal cell heterogeneous response |
| topic | Bisphenol analogues Intestinal cell heterogeneity Metabolic effect Hepatic steatosis Insulin resistance Zebrafish |
| url | http://www.sciencedirect.com/science/article/pii/S0160412022002148 |
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