“Smart” Polylactic Acid Films with Ceftriaxone Loaded Microchamber Arrays for Personalized Antibiotic Therapy
Bacterial infections are a severe medical problem, especially in traumatology, orthopedics, and surgery. The local use of antibiotics-elution materials has made it possible to increase the effectiveness of acute infections treatment. However, the infection prevention problem remains unresolved. Here...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/1/42 |
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| author | Ekaterina A. Mordovina Valentina O. Plastun Arkady S. Abdurashitov Pavel I. Proshin Svetlana V. Raikova Daniil N. Bratashov Olga A. Inozemtseva Irina Yu. Goryacheva Gleb B. Sukhorukov Olga A. Sindeeva |
| author_facet | Ekaterina A. Mordovina Valentina O. Plastun Arkady S. Abdurashitov Pavel I. Proshin Svetlana V. Raikova Daniil N. Bratashov Olga A. Inozemtseva Irina Yu. Goryacheva Gleb B. Sukhorukov Olga A. Sindeeva |
| author_sort | Ekaterina A. Mordovina |
| collection | DOAJ |
| description | Bacterial infections are a severe medical problem, especially in traumatology, orthopedics, and surgery. The local use of antibiotics-elution materials has made it possible to increase the effectiveness of acute infections treatment. However, the infection prevention problem remains unresolved. Here, we demonstrate the fabrication of polylactic acid (PLA) “smart” films with microchamber arrays. These microchambers contain ceftriaxone as a payload in concentrations ranging from 12 ± 1 μg/cm<sup>2</sup> to 38 ± 8 μg/cm<sup>2</sup>, depending on the patterned film thickness formed by the different PLA concentrations in chloroform. In addition, the release profile of the antibiotic can be prolonged up to 72 h in saline. At the same time, on the surface of agar plates, the antibiotic release time increases up to 96 h, which has been confirmed by the growth suppression of the <i>Staphylococcus aureus</i> bacteria. The efficient loading and optimal release rate are obtained for patterned films formed by the 1.5 wt % PLA in chloroform. The films produced from 1.5 and 2 wt % PLA solutions (thickness—0.42 ± 0.12 and 0.68 ± 0.16 µm, respectively) show an accelerated ceftriaxone release upon the trigger of the therapeutic ultrasound, which impacted as an expansion of the bacterial growth inhibition zone around the samples. Combining prolonged drug elution with the on-demand release ability of large cargo amount opens up new approaches for personalized and custom-tunable antibacterial therapy. |
| first_indexed | 2024-03-10T00:44:37Z |
| format | Article |
| id | doaj.art-e933382b82da46109ff2ed9e3cdf5657 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T00:44:37Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-e933382b82da46109ff2ed9e3cdf56572023-11-23T15:02:56ZengMDPI AGPharmaceutics1999-49232021-12-011414210.3390/pharmaceutics14010042“Smart” Polylactic Acid Films with Ceftriaxone Loaded Microchamber Arrays for Personalized Antibiotic TherapyEkaterina A. Mordovina0Valentina O. Plastun1Arkady S. Abdurashitov2Pavel I. Proshin3Svetlana V. Raikova4Daniil N. Bratashov5Olga A. Inozemtseva6Irina Yu. Goryacheva7Gleb B. Sukhorukov8Olga A. Sindeeva9Science Medical Center, Saratov State University, 83 Astrakhanskaya Str., 410012 Saratov, RussiaScience Medical Center, Saratov State University, 83 Astrakhanskaya Str., 410012 Saratov, RussiaCenter for Neurobiology and Brain Restoration, Skolkovo Institute of Science and Technology, 3 Nobel Str., 143005 Moscow, RussiaCenter for Neurobiology and Brain Restoration, Skolkovo Institute of Science and Technology, 3 Nobel Str., 143005 Moscow, RussiaSaratov Hygiene Medical Research Center of the FBSI «FSC Medical and Preventive Health Risk Management Technologies», 1A Zarechnaya Str., 410022 Saratov, RussiaScience Medical Center, Saratov State University, 83 Astrakhanskaya Str., 410012 Saratov, RussiaScience Medical Center, Saratov State University, 83 Astrakhanskaya Str., 410012 Saratov, RussiaScience Medical Center, Saratov State University, 83 Astrakhanskaya Str., 410012 Saratov, RussiaCenter for Neurobiology and Brain Restoration, Skolkovo Institute of Science and Technology, 3 Nobel Str., 143005 Moscow, RussiaCenter for Neurobiology and Brain Restoration, Skolkovo Institute of Science and Technology, 3 Nobel Str., 143005 Moscow, RussiaBacterial infections are a severe medical problem, especially in traumatology, orthopedics, and surgery. The local use of antibiotics-elution materials has made it possible to increase the effectiveness of acute infections treatment. However, the infection prevention problem remains unresolved. Here, we demonstrate the fabrication of polylactic acid (PLA) “smart” films with microchamber arrays. These microchambers contain ceftriaxone as a payload in concentrations ranging from 12 ± 1 μg/cm<sup>2</sup> to 38 ± 8 μg/cm<sup>2</sup>, depending on the patterned film thickness formed by the different PLA concentrations in chloroform. In addition, the release profile of the antibiotic can be prolonged up to 72 h in saline. At the same time, on the surface of agar plates, the antibiotic release time increases up to 96 h, which has been confirmed by the growth suppression of the <i>Staphylococcus aureus</i> bacteria. The efficient loading and optimal release rate are obtained for patterned films formed by the 1.5 wt % PLA in chloroform. The films produced from 1.5 and 2 wt % PLA solutions (thickness—0.42 ± 0.12 and 0.68 ± 0.16 µm, respectively) show an accelerated ceftriaxone release upon the trigger of the therapeutic ultrasound, which impacted as an expansion of the bacterial growth inhibition zone around the samples. Combining prolonged drug elution with the on-demand release ability of large cargo amount opens up new approaches for personalized and custom-tunable antibacterial therapy.https://www.mdpi.com/1999-4923/14/1/42prolonged releaseultrasound-induced releasedrug deliverymicrochamber arraysPLAantibiotics |
| spellingShingle | Ekaterina A. Mordovina Valentina O. Plastun Arkady S. Abdurashitov Pavel I. Proshin Svetlana V. Raikova Daniil N. Bratashov Olga A. Inozemtseva Irina Yu. Goryacheva Gleb B. Sukhorukov Olga A. Sindeeva “Smart” Polylactic Acid Films with Ceftriaxone Loaded Microchamber Arrays for Personalized Antibiotic Therapy Pharmaceutics prolonged release ultrasound-induced release drug delivery microchamber arrays PLA antibiotics |
| title | “Smart” Polylactic Acid Films with Ceftriaxone Loaded Microchamber Arrays for Personalized Antibiotic Therapy |
| title_full | “Smart” Polylactic Acid Films with Ceftriaxone Loaded Microchamber Arrays for Personalized Antibiotic Therapy |
| title_fullStr | “Smart” Polylactic Acid Films with Ceftriaxone Loaded Microchamber Arrays for Personalized Antibiotic Therapy |
| title_full_unstemmed | “Smart” Polylactic Acid Films with Ceftriaxone Loaded Microchamber Arrays for Personalized Antibiotic Therapy |
| title_short | “Smart” Polylactic Acid Films with Ceftriaxone Loaded Microchamber Arrays for Personalized Antibiotic Therapy |
| title_sort | smart polylactic acid films with ceftriaxone loaded microchamber arrays for personalized antibiotic therapy |
| topic | prolonged release ultrasound-induced release drug delivery microchamber arrays PLA antibiotics |
| url | https://www.mdpi.com/1999-4923/14/1/42 |
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