In Situ Bioremediation in Mixed-culture Inoculated Biological Permeable Reactive Barrier Systems
Cr(VI) reducing bacteria was isolated from dried sludge collected from sand drying beds at a local wastewater treatment plant in Brits (South Africa). The plant received high periodic loadings of Cr(VI) contaminated effluent from an abandoned chrome processing foundry within the chrome mining town o...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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AIDIC Servizi S.r.l.
2023-11-01
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| Series: | Chemical Engineering Transactions |
| Online Access: | http://www.cetjournal.it/index.php/cet/article/view/13804 |
| _version_ | 1827622819117137920 |
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| author | Evans M.N. Chirwa Karisa Naidu Buyisile Kholisa Mpumelello T. Matsena Boitumelo F. Mashangoane Pulane E. Molokwane |
| author_facet | Evans M.N. Chirwa Karisa Naidu Buyisile Kholisa Mpumelello T. Matsena Boitumelo F. Mashangoane Pulane E. Molokwane |
| author_sort | Evans M.N. Chirwa |
| collection | DOAJ |
| description | Cr(VI) reducing bacteria was isolated from dried sludge collected from sand drying beds at a local wastewater treatment plant in Brits (South Africa). The plant received high periodic loadings of Cr(VI) contaminated effluent from an abandoned chrome processing foundry within the chrome mining town of Brits. The isolated bacteria were tolerant to high Cr(VI) loadings with significant chromium removal activity at loadings up to 80 mg(L-1 under continuous flow conditions. The active species in the sludge culture were determined to be predominated by Bacillus thirungiensis, Bacillus cereus, Lycinobacillus sphaerococcus. The isolated consortium culture was introduced as a biocatalyst in a microbial permeable bioreactive barrier for treatment of Cr(VI) contaminated water through soil medium. When tested in continuous flow bench-scale systems, the steady-state condition was attained after the reactor's operation for 25 days. Time course concentration profiles in batch tests fitted well with first- and second-order exponential rate equations yielding first-order rate constants in the range of 0.615 h-1 and 0.0532 L(mg-1(h-1 for Cr(VI) loadings ranging from 50 to 400 mg/L. The laboratory scale studies showed that the biological permeable reactive barrier technology using indigenous microbes has potential application for hexavalent chromium remediation in contaminated environments. Finally, the technology using bacteria in-situ shows that Cr(VI) can be remediated in the environment using a passive system at a low cost with minimum intervention. |
| first_indexed | 2024-03-09T11:33:05Z |
| format | Article |
| id | doaj.art-e933b4611f054da08a85beb15feef10c |
| institution | Directory Open Access Journal |
| issn | 2283-9216 |
| language | English |
| last_indexed | 2024-03-09T11:33:05Z |
| publishDate | 2023-11-01 |
| publisher | AIDIC Servizi S.r.l. |
| record_format | Article |
| series | Chemical Engineering Transactions |
| spelling | doaj.art-e933b4611f054da08a85beb15feef10c2023-11-30T23:48:55ZengAIDIC Servizi S.r.l.Chemical Engineering Transactions2283-92162023-11-0110510.3303/CET23105066In Situ Bioremediation in Mixed-culture Inoculated Biological Permeable Reactive Barrier SystemsEvans M.N. ChirwaKarisa NaiduBuyisile KholisaMpumelello T. MatsenaBoitumelo F. MashangoanePulane E. MolokwaneCr(VI) reducing bacteria was isolated from dried sludge collected from sand drying beds at a local wastewater treatment plant in Brits (South Africa). The plant received high periodic loadings of Cr(VI) contaminated effluent from an abandoned chrome processing foundry within the chrome mining town of Brits. The isolated bacteria were tolerant to high Cr(VI) loadings with significant chromium removal activity at loadings up to 80 mg(L-1 under continuous flow conditions. The active species in the sludge culture were determined to be predominated by Bacillus thirungiensis, Bacillus cereus, Lycinobacillus sphaerococcus. The isolated consortium culture was introduced as a biocatalyst in a microbial permeable bioreactive barrier for treatment of Cr(VI) contaminated water through soil medium. When tested in continuous flow bench-scale systems, the steady-state condition was attained after the reactor's operation for 25 days. Time course concentration profiles in batch tests fitted well with first- and second-order exponential rate equations yielding first-order rate constants in the range of 0.615 h-1 and 0.0532 L(mg-1(h-1 for Cr(VI) loadings ranging from 50 to 400 mg/L. The laboratory scale studies showed that the biological permeable reactive barrier technology using indigenous microbes has potential application for hexavalent chromium remediation in contaminated environments. Finally, the technology using bacteria in-situ shows that Cr(VI) can be remediated in the environment using a passive system at a low cost with minimum intervention.http://www.cetjournal.it/index.php/cet/article/view/13804 |
| spellingShingle | Evans M.N. Chirwa Karisa Naidu Buyisile Kholisa Mpumelello T. Matsena Boitumelo F. Mashangoane Pulane E. Molokwane In Situ Bioremediation in Mixed-culture Inoculated Biological Permeable Reactive Barrier Systems Chemical Engineering Transactions |
| title | In Situ Bioremediation in Mixed-culture Inoculated Biological Permeable Reactive Barrier Systems |
| title_full | In Situ Bioremediation in Mixed-culture Inoculated Biological Permeable Reactive Barrier Systems |
| title_fullStr | In Situ Bioremediation in Mixed-culture Inoculated Biological Permeable Reactive Barrier Systems |
| title_full_unstemmed | In Situ Bioremediation in Mixed-culture Inoculated Biological Permeable Reactive Barrier Systems |
| title_short | In Situ Bioremediation in Mixed-culture Inoculated Biological Permeable Reactive Barrier Systems |
| title_sort | in situ bioremediation in mixed culture inoculated biological permeable reactive barrier systems |
| url | http://www.cetjournal.it/index.php/cet/article/view/13804 |
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