Foxtail millet supplementation improves glucose metabolism and gut microbiota in rats with high-fat diet/streptozotocin-induced diabetes
Foxtail millet (FM) whole grain has received special attention in recent years. To confirm the hypoglycemic effects of FM, we investigated the effects of FM supplementation on glucose metabolism and gut microbiota in rats with high-fat diet/streptozotocin (HFD/STZ)-induced diabetes. Specifically, we...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2022-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453021000793 |
| _version_ | 1797705223388528640 |
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| author | Xin Ren Linxuan Wang Zenglong Chen Min Zhang Dianzhi Hou Yong Xue Xianmin Diao Ruihai Liu Qun Shen |
| author_facet | Xin Ren Linxuan Wang Zenglong Chen Min Zhang Dianzhi Hou Yong Xue Xianmin Diao Ruihai Liu Qun Shen |
| author_sort | Xin Ren |
| collection | DOAJ |
| description | Foxtail millet (FM) whole grain has received special attention in recent years. To confirm the hypoglycemic effects of FM, we investigated the effects of FM supplementation on glucose metabolism and gut microbiota in rats with high-fat diet/streptozotocin (HFD/STZ)-induced diabetes. Specifically, we fully assessed the blood biochemical profiles, pancreatic histopathology, insulin-glucagon immunofluorescence, short-chain fatty acids, and gut microbiota composition of rats with HFD/STZ-induced diabetes before and after FM supplementation. Results showed that both 30% and 48% FM supplementation significantly decreased concentrations of fasting blood glucose, 60-min blood glucose, and blood triglycerides (P < 0.05); additionally, 48% FM supplementation significantly improved blood glucose tolerance and insulin resistance (P < 0.05). However, FM supplementation could not effectively repair damage to β-cells over a short period of time. In addition, 4 weeks of 48% FM supplementation siginificantly increased the relative abundance of Bifidobacterium and concentration of butyrate, suggesting that the hypoglycemic effects of FM supplementation might be partially mediated by gut microbiota. Collectively, we found a dose-dependent relationship between FM supplementation and improvement of blood glucose metabolism, but did not find a synergistic effect between FM supplementation and metformin (Met) treatment. Our findings provide further support that consuming more whole-grain FM might be beneficial to individuals suffering from type 2 diabetes. |
| first_indexed | 2024-03-12T05:32:58Z |
| format | Article |
| id | doaj.art-e9356080dcf44373a2d929479fc3367c |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T05:32:58Z |
| publishDate | 2022-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj.art-e9356080dcf44373a2d929479fc3367c2023-09-03T06:43:16ZengTsinghua University PressFood Science and Human Wellness2213-45302022-01-01111119128Foxtail millet supplementation improves glucose metabolism and gut microbiota in rats with high-fat diet/streptozotocin-induced diabetesXin Ren0Linxuan Wang1Zenglong Chen2Min Zhang3Dianzhi Hou4Yong Xue5Xianmin Diao6Ruihai Liu7Qun Shen8Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, China; Key Laboratory of Plant Protein and Grain processing, National Engineering and Technology Research Center for Fruits and Vegetables, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, ChinaState Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, ChinaKey Laboratory of Plant Protein and Grain processing, National Engineering and Technology Research Center for Fruits and Vegetables, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaKey Laboratory of Plant Protein and Grain processing, National Engineering and Technology Research Center for Fruits and Vegetables, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaChinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Comparative and Environmental Toxicology, Department of Food Science, Cornell University, Ithaca, NY, United StatesKey Laboratory of Plant Protein and Grain processing, National Engineering and Technology Research Center for Fruits and Vegetables, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; Corresponding author at: College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.Foxtail millet (FM) whole grain has received special attention in recent years. To confirm the hypoglycemic effects of FM, we investigated the effects of FM supplementation on glucose metabolism and gut microbiota in rats with high-fat diet/streptozotocin (HFD/STZ)-induced diabetes. Specifically, we fully assessed the blood biochemical profiles, pancreatic histopathology, insulin-glucagon immunofluorescence, short-chain fatty acids, and gut microbiota composition of rats with HFD/STZ-induced diabetes before and after FM supplementation. Results showed that both 30% and 48% FM supplementation significantly decreased concentrations of fasting blood glucose, 60-min blood glucose, and blood triglycerides (P < 0.05); additionally, 48% FM supplementation significantly improved blood glucose tolerance and insulin resistance (P < 0.05). However, FM supplementation could not effectively repair damage to β-cells over a short period of time. In addition, 4 weeks of 48% FM supplementation siginificantly increased the relative abundance of Bifidobacterium and concentration of butyrate, suggesting that the hypoglycemic effects of FM supplementation might be partially mediated by gut microbiota. Collectively, we found a dose-dependent relationship between FM supplementation and improvement of blood glucose metabolism, but did not find a synergistic effect between FM supplementation and metformin (Met) treatment. Our findings provide further support that consuming more whole-grain FM might be beneficial to individuals suffering from type 2 diabetes.http://www.sciencedirect.com/science/article/pii/S2213453021000793Foxtail milletGlucose metabolismGut microbiotaShort-chain fatty acids |
| spellingShingle | Xin Ren Linxuan Wang Zenglong Chen Min Zhang Dianzhi Hou Yong Xue Xianmin Diao Ruihai Liu Qun Shen Foxtail millet supplementation improves glucose metabolism and gut microbiota in rats with high-fat diet/streptozotocin-induced diabetes Food Science and Human Wellness Foxtail millet Glucose metabolism Gut microbiota Short-chain fatty acids |
| title | Foxtail millet supplementation improves glucose metabolism and gut microbiota in rats with high-fat diet/streptozotocin-induced diabetes |
| title_full | Foxtail millet supplementation improves glucose metabolism and gut microbiota in rats with high-fat diet/streptozotocin-induced diabetes |
| title_fullStr | Foxtail millet supplementation improves glucose metabolism and gut microbiota in rats with high-fat diet/streptozotocin-induced diabetes |
| title_full_unstemmed | Foxtail millet supplementation improves glucose metabolism and gut microbiota in rats with high-fat diet/streptozotocin-induced diabetes |
| title_short | Foxtail millet supplementation improves glucose metabolism and gut microbiota in rats with high-fat diet/streptozotocin-induced diabetes |
| title_sort | foxtail millet supplementation improves glucose metabolism and gut microbiota in rats with high fat diet streptozotocin induced diabetes |
| topic | Foxtail millet Glucose metabolism Gut microbiota Short-chain fatty acids |
| url | http://www.sciencedirect.com/science/article/pii/S2213453021000793 |
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