ASIC3 plays a protective role in delayed-onset muscle soreness (DOMS) through muscle acid sensation during exercise
Immediate exercise-induced pain (IEIP) and DOMS are two types of exercise-induced muscle pain and can act as barriers to exercise. The burning sensation of IEIP occurs during and immediately after intensive exercise, whereas the soreness of DOMS occurs later. Acid-sensing ion channels (ASICs) within...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-09-01
|
| Series: | Frontiers in Pain Research |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpain.2023.1215197/full |
| _version_ | 1797680863413010432 |
|---|---|
| author | Tahsin Khataei Tahsin Khataei Tahsin Khataei Christopher J. Benson Christopher J. Benson |
| author_facet | Tahsin Khataei Tahsin Khataei Tahsin Khataei Christopher J. Benson Christopher J. Benson |
| author_sort | Tahsin Khataei |
| collection | DOAJ |
| description | Immediate exercise-induced pain (IEIP) and DOMS are two types of exercise-induced muscle pain and can act as barriers to exercise. The burning sensation of IEIP occurs during and immediately after intensive exercise, whereas the soreness of DOMS occurs later. Acid-sensing ion channels (ASICs) within muscle afferents are activated by H+ and other chemicals and have been shown to play a role in various chronic muscle pain conditions. Here, we further defined the role of ASICs in IEIP, and also tested if ASIC3 is required for DOMS. After undergoing exhaustive treadmill exercise, exercise-induced muscle pain was assessed in wild-type (WT) and ASIC3−/− mice at baseline via muscle withdrawal threshold (MWT), immediately, and 24 h after exercise. Locomotor movement, grip strength, and repeat exercise performance were tested at baseline and 24 h after exercise to evaluate DOMS. We found that ASIC3−/− had similar baseline muscle pain, locomotor activity, grip strength, and exercise performance as WT mice. WT showed diminished MWT immediately after exercise indicating they developed IEIP, but ASIC3−/− mice did not. At 24 h after baseline exercise, both ASIC3−/− and WT had similarly lower MWT and grip strength, however, ASIC3−/− displayed significantly lower locomotor activity and repeat exercise performance at 24 h time points compared to WT. In addition, ASIC3−/− mice had higher muscle injury as measured by serum lactate dehydrogenase and creatine kinase levels at 24 h after exercise. These results show that ASIC3 is required for IEIP, but not DOMS, and in fact might play a protective role to prevent muscle injury associated with strenuous exercise. |
| first_indexed | 2024-03-11T23:36:26Z |
| format | Article |
| id | doaj.art-e938f154b2ea4ff39fe9f41bcdca3dfc |
| institution | Directory Open Access Journal |
| issn | 2673-561X |
| language | English |
| last_indexed | 2024-03-11T23:36:26Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pain Research |
| spelling | doaj.art-e938f154b2ea4ff39fe9f41bcdca3dfc2023-09-19T23:03:46ZengFrontiers Media S.A.Frontiers in Pain Research2673-561X2023-09-01410.3389/fpain.2023.12151971215197ASIC3 plays a protective role in delayed-onset muscle soreness (DOMS) through muscle acid sensation during exerciseTahsin Khataei0Tahsin Khataei1Tahsin Khataei2Christopher J. Benson3Christopher J. Benson4Department of Internal Medicine, Roy J and Lucile A. Carver College of Medicine, University of Iowa, Iowa City, IA, United StatesIowa City VA Healthcare System, Iowa City, IA, United StatesDepartment of Health and Human Physiology, University of Iowa, Iowa City, IA, United StatesDepartment of Internal Medicine, Roy J and Lucile A. Carver College of Medicine, University of Iowa, Iowa City, IA, United StatesIowa City VA Healthcare System, Iowa City, IA, United StatesImmediate exercise-induced pain (IEIP) and DOMS are two types of exercise-induced muscle pain and can act as barriers to exercise. The burning sensation of IEIP occurs during and immediately after intensive exercise, whereas the soreness of DOMS occurs later. Acid-sensing ion channels (ASICs) within muscle afferents are activated by H+ and other chemicals and have been shown to play a role in various chronic muscle pain conditions. Here, we further defined the role of ASICs in IEIP, and also tested if ASIC3 is required for DOMS. After undergoing exhaustive treadmill exercise, exercise-induced muscle pain was assessed in wild-type (WT) and ASIC3−/− mice at baseline via muscle withdrawal threshold (MWT), immediately, and 24 h after exercise. Locomotor movement, grip strength, and repeat exercise performance were tested at baseline and 24 h after exercise to evaluate DOMS. We found that ASIC3−/− had similar baseline muscle pain, locomotor activity, grip strength, and exercise performance as WT mice. WT showed diminished MWT immediately after exercise indicating they developed IEIP, but ASIC3−/− mice did not. At 24 h after baseline exercise, both ASIC3−/− and WT had similarly lower MWT and grip strength, however, ASIC3−/− displayed significantly lower locomotor activity and repeat exercise performance at 24 h time points compared to WT. In addition, ASIC3−/− mice had higher muscle injury as measured by serum lactate dehydrogenase and creatine kinase levels at 24 h after exercise. These results show that ASIC3 is required for IEIP, but not DOMS, and in fact might play a protective role to prevent muscle injury associated with strenuous exercise.https://www.frontiersin.org/articles/10.3389/fpain.2023.1215197/fullASICsmuscle afferentsensory neuronexercisepaindelayed-onset muscle soreness |
| spellingShingle | Tahsin Khataei Tahsin Khataei Tahsin Khataei Christopher J. Benson Christopher J. Benson ASIC3 plays a protective role in delayed-onset muscle soreness (DOMS) through muscle acid sensation during exercise Frontiers in Pain Research ASICs muscle afferent sensory neuron exercise pain delayed-onset muscle soreness |
| title | ASIC3 plays a protective role in delayed-onset muscle soreness (DOMS) through muscle acid sensation during exercise |
| title_full | ASIC3 plays a protective role in delayed-onset muscle soreness (DOMS) through muscle acid sensation during exercise |
| title_fullStr | ASIC3 plays a protective role in delayed-onset muscle soreness (DOMS) through muscle acid sensation during exercise |
| title_full_unstemmed | ASIC3 plays a protective role in delayed-onset muscle soreness (DOMS) through muscle acid sensation during exercise |
| title_short | ASIC3 plays a protective role in delayed-onset muscle soreness (DOMS) through muscle acid sensation during exercise |
| title_sort | asic3 plays a protective role in delayed onset muscle soreness doms through muscle acid sensation during exercise |
| topic | ASICs muscle afferent sensory neuron exercise pain delayed-onset muscle soreness |
| url | https://www.frontiersin.org/articles/10.3389/fpain.2023.1215197/full |
| work_keys_str_mv | AT tahsinkhataei asic3playsaprotectiveroleindelayedonsetmusclesorenessdomsthroughmuscleacidsensationduringexercise AT tahsinkhataei asic3playsaprotectiveroleindelayedonsetmusclesorenessdomsthroughmuscleacidsensationduringexercise AT tahsinkhataei asic3playsaprotectiveroleindelayedonsetmusclesorenessdomsthroughmuscleacidsensationduringexercise AT christopherjbenson asic3playsaprotectiveroleindelayedonsetmusclesorenessdomsthroughmuscleacidsensationduringexercise AT christopherjbenson asic3playsaprotectiveroleindelayedonsetmusclesorenessdomsthroughmuscleacidsensationduringexercise |