Taxonomic and Biochemical Characterization of Microalga <i>Graesiella emersonii</i> GEGS21 for Its Potential to Become Feedstock for Biofuels and Bioproducts
<i>Graesiella emersonii</i> is a commercially exploitable source of bioactive compounds and biofuels with potential applications in microalgae-based industries. Despite this, little taxonomical information is available. Therefore, proper identification and characterization are needed for...
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MDPI AG
2022-11-01
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| Series: | Energies |
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| Online Access: | https://www.mdpi.com/1996-1073/15/22/8725 |
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| author | Nam Seon Kang Kichul Cho Sung Min An Eun Song Kim Hyunji Ki Chung Hyeon Lee Grace Choi Ji Won Hong |
| author_facet | Nam Seon Kang Kichul Cho Sung Min An Eun Song Kim Hyunji Ki Chung Hyeon Lee Grace Choi Ji Won Hong |
| author_sort | Nam Seon Kang |
| collection | DOAJ |
| description | <i>Graesiella emersonii</i> is a commercially exploitable source of bioactive compounds and biofuels with potential applications in microalgae-based industries. Despite this, little taxonomical information is available. Therefore, proper identification and characterization are needed for the sustainable utilization of isolated microalgae. In this study, an axenically isolated unicellular green alga from the Geumgang Estuary, Korea was investigated for its morphological, molecular, and biochemical characteristics. The morphological characteristics were typical of <i>G. emersonii.</i> Molecular phylogenetic analysis of the 18S rDNA sequence verified that the isolate belonged to <i>G. emersonii</i> and was subsequently named <i>G. emersonii</i> GEGS21. It was isolated from brackish water, and its optimal growth temperature, salinity, and light intensity were at 28–32 °C, 0 M NaCl, and 130–160 µmol m<sup>−2</sup> s<sup>−1</sup>, respectively. The strain thrived over a range of temperatures (5–40 °C) and withstood up to 0.5 M NaCl. The isolate was rich in omega-6 linoleic acid (C<sub>18:2</sub> n-6, 26.3%) and palmitic acid (C<sub>16:0</sub>, 27.5%). The fuel quality properties were determined, and biodiesel from GEGS21 could be used as a biodiesel blend. Value-added carotenoids lutein (1.5 mg g<sup>−1</sup> dry cell weight, DCW) and neoxanthin (1.2 mg g<sup>−1</sup> DCW) were biosynthesized as accessory pigments by this microalga. The biomass of this microalga may serve as feedstock for biodiesel production as well as producing valuable ω-6 and carotenoids. |
| first_indexed | 2024-03-09T18:22:02Z |
| format | Article |
| id | doaj.art-e93958ec08934c9380966b5475c29a89 |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-03-09T18:22:02Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-e93958ec08934c9380966b5475c29a892023-11-24T08:17:34ZengMDPI AGEnergies1996-10732022-11-011522872510.3390/en15228725Taxonomic and Biochemical Characterization of Microalga <i>Graesiella emersonii</i> GEGS21 for Its Potential to Become Feedstock for Biofuels and BioproductsNam Seon Kang0Kichul Cho1Sung Min An2Eun Song Kim3Hyunji Ki4Chung Hyeon Lee5Grace Choi6Ji Won Hong7Department of Microbial Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of KoreaDepartment of Microbial Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of KoreaDepartment of Microbial Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of KoreaDepartment of Microbial Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of KoreaDepartment of Microbial Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of KoreaDepartment of Microbial Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of KoreaDepartment of Microbial Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of KoreaDepartment of Hydrogen and Renewable Energy, Kyungpook National University, Daegu 41566, Republic of Korea<i>Graesiella emersonii</i> is a commercially exploitable source of bioactive compounds and biofuels with potential applications in microalgae-based industries. Despite this, little taxonomical information is available. Therefore, proper identification and characterization are needed for the sustainable utilization of isolated microalgae. In this study, an axenically isolated unicellular green alga from the Geumgang Estuary, Korea was investigated for its morphological, molecular, and biochemical characteristics. The morphological characteristics were typical of <i>G. emersonii.</i> Molecular phylogenetic analysis of the 18S rDNA sequence verified that the isolate belonged to <i>G. emersonii</i> and was subsequently named <i>G. emersonii</i> GEGS21. It was isolated from brackish water, and its optimal growth temperature, salinity, and light intensity were at 28–32 °C, 0 M NaCl, and 130–160 µmol m<sup>−2</sup> s<sup>−1</sup>, respectively. The strain thrived over a range of temperatures (5–40 °C) and withstood up to 0.5 M NaCl. The isolate was rich in omega-6 linoleic acid (C<sub>18:2</sub> n-6, 26.3%) and palmitic acid (C<sub>16:0</sub>, 27.5%). The fuel quality properties were determined, and biodiesel from GEGS21 could be used as a biodiesel blend. Value-added carotenoids lutein (1.5 mg g<sup>−1</sup> dry cell weight, DCW) and neoxanthin (1.2 mg g<sup>−1</sup> DCW) were biosynthesized as accessory pigments by this microalga. The biomass of this microalga may serve as feedstock for biodiesel production as well as producing valuable ω-6 and carotenoids.https://www.mdpi.com/1996-1073/15/22/8725<i>Graesiella emersonii</i>luteintaxonomypigmentsfatty acidsomega-6 |
| spellingShingle | Nam Seon Kang Kichul Cho Sung Min An Eun Song Kim Hyunji Ki Chung Hyeon Lee Grace Choi Ji Won Hong Taxonomic and Biochemical Characterization of Microalga <i>Graesiella emersonii</i> GEGS21 for Its Potential to Become Feedstock for Biofuels and Bioproducts Energies <i>Graesiella emersonii</i> lutein taxonomy pigments fatty acids omega-6 |
| title | Taxonomic and Biochemical Characterization of Microalga <i>Graesiella emersonii</i> GEGS21 for Its Potential to Become Feedstock for Biofuels and Bioproducts |
| title_full | Taxonomic and Biochemical Characterization of Microalga <i>Graesiella emersonii</i> GEGS21 for Its Potential to Become Feedstock for Biofuels and Bioproducts |
| title_fullStr | Taxonomic and Biochemical Characterization of Microalga <i>Graesiella emersonii</i> GEGS21 for Its Potential to Become Feedstock for Biofuels and Bioproducts |
| title_full_unstemmed | Taxonomic and Biochemical Characterization of Microalga <i>Graesiella emersonii</i> GEGS21 for Its Potential to Become Feedstock for Biofuels and Bioproducts |
| title_short | Taxonomic and Biochemical Characterization of Microalga <i>Graesiella emersonii</i> GEGS21 for Its Potential to Become Feedstock for Biofuels and Bioproducts |
| title_sort | taxonomic and biochemical characterization of microalga i graesiella emersonii i gegs21 for its potential to become feedstock for biofuels and bioproducts |
| topic | <i>Graesiella emersonii</i> lutein taxonomy pigments fatty acids omega-6 |
| url | https://www.mdpi.com/1996-1073/15/22/8725 |
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