Application of microcalorimetry of Escherichia coli growth and discriminant analysis to the quality assessment of a Chinese herbal injection (Yinzhihuang)
This paper describes a novel approach to the quality control of a Chinese herbal injection based on microcalorimetric determination of its effect on the biothermal active fingerprint (BTAF) of Escherichia coli (E. coli) growth. Yinzhihuang Injection (YZHI) was selected for this proof of concept stud...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2012-06-01
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| Series: | Acta Pharmaceutica Sinica B |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211383512000524 |
| _version_ | 1818993484046008320 |
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| author | Yongshen Ren Ping Zhang Dan Yan Yan Yan Longhu Chen Lingling Qiu Zhinan Mei Xiaohe Xiao |
| author_facet | Yongshen Ren Ping Zhang Dan Yan Yan Yan Longhu Chen Lingling Qiu Zhinan Mei Xiaohe Xiao |
| author_sort | Yongshen Ren |
| collection | DOAJ |
| description | This paper describes a novel approach to the quality control of a Chinese herbal injection based on microcalorimetric determination of its effect on the biothermal active fingerprint (BTAF) of Escherichia coli (E. coli) growth. Yinzhihuang Injection (YZHI) was selected for this proof of concept study. Reference samples of YZHI were collected and compared with test (stressed) samples prepared under different stress conditions. The BTAF of E. coli growth was found to be affected by YZHI and the changes were analyzed on the basis of eleven biothermokinetic parameters. Similarity and multivariate statistical analysis were used to investigate the differences between reference and test samples and discriminant analysis was used to delineate the altered samples. Reference samples were found to have coincident BTAFs with similarity index >0.99. Stressed samples showed differences in the BTAF which increased in line with decreased quality. Discriminant formulae were developed based on a sensitivity parameter which could identify all altered samples. In conclusion, BTAF can be used to assess the quality of YZHI both qualitatively and quantitatively and has the potential to provide a sensitive method for quality control of Chinese herbal injections. |
| first_indexed | 2024-12-20T20:42:47Z |
| format | Article |
| id | doaj.art-e93ac7c9e8e0488f869650bf54812a1a |
| institution | Directory Open Access Journal |
| issn | 2211-3835 2211-3843 |
| language | English |
| last_indexed | 2024-12-20T20:42:47Z |
| publishDate | 2012-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Acta Pharmaceutica Sinica B |
| spelling | doaj.art-e93ac7c9e8e0488f869650bf54812a1a2022-12-21T19:27:07ZengElsevierActa Pharmaceutica Sinica B2211-38352211-38432012-06-012327828510.1016/j.apsb.2012.02.013Application of microcalorimetry of Escherichia coli growth and discriminant analysis to the quality assessment of a Chinese herbal injection (Yinzhihuang)Yongshen Ren0Ping Zhang1Dan Yan2Yan Yan3Longhu Chen4Lingling Qiu5Zhinan Mei6Xiaohe Xiao7China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, ChinaChina Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, ChinaChina Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, ChinaChina Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, ChinaChina Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, ChinaChina Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, ChinaPharmacy College, South-Central University for Nationalities, Wuhan 430074, ChinaChina Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, ChinaThis paper describes a novel approach to the quality control of a Chinese herbal injection based on microcalorimetric determination of its effect on the biothermal active fingerprint (BTAF) of Escherichia coli (E. coli) growth. Yinzhihuang Injection (YZHI) was selected for this proof of concept study. Reference samples of YZHI were collected and compared with test (stressed) samples prepared under different stress conditions. The BTAF of E. coli growth was found to be affected by YZHI and the changes were analyzed on the basis of eleven biothermokinetic parameters. Similarity and multivariate statistical analysis were used to investigate the differences between reference and test samples and discriminant analysis was used to delineate the altered samples. Reference samples were found to have coincident BTAFs with similarity index >0.99. Stressed samples showed differences in the BTAF which increased in line with decreased quality. Discriminant formulae were developed based on a sensitivity parameter which could identify all altered samples. In conclusion, BTAF can be used to assess the quality of YZHI both qualitatively and quantitatively and has the potential to provide a sensitive method for quality control of Chinese herbal injections.http://www.sciencedirect.com/science/article/pii/S2211383512000524Biothermal active fingerprintMicrocalorimetryQuality controlChinese herbal injectionEscherichia coli |
| spellingShingle | Yongshen Ren Ping Zhang Dan Yan Yan Yan Longhu Chen Lingling Qiu Zhinan Mei Xiaohe Xiao Application of microcalorimetry of Escherichia coli growth and discriminant analysis to the quality assessment of a Chinese herbal injection (Yinzhihuang) Acta Pharmaceutica Sinica B Biothermal active fingerprint Microcalorimetry Quality control Chinese herbal injection Escherichia coli |
| title | Application of microcalorimetry of Escherichia coli growth and discriminant analysis to the quality assessment of a Chinese herbal injection (Yinzhihuang) |
| title_full | Application of microcalorimetry of Escherichia coli growth and discriminant analysis to the quality assessment of a Chinese herbal injection (Yinzhihuang) |
| title_fullStr | Application of microcalorimetry of Escherichia coli growth and discriminant analysis to the quality assessment of a Chinese herbal injection (Yinzhihuang) |
| title_full_unstemmed | Application of microcalorimetry of Escherichia coli growth and discriminant analysis to the quality assessment of a Chinese herbal injection (Yinzhihuang) |
| title_short | Application of microcalorimetry of Escherichia coli growth and discriminant analysis to the quality assessment of a Chinese herbal injection (Yinzhihuang) |
| title_sort | application of microcalorimetry of escherichia coli growth and discriminant analysis to the quality assessment of a chinese herbal injection yinzhihuang |
| topic | Biothermal active fingerprint Microcalorimetry Quality control Chinese herbal injection Escherichia coli |
| url | http://www.sciencedirect.com/science/article/pii/S2211383512000524 |
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