Isolation, Structural Elucidation, Antioxidant and Hypoglycemic Activity of Polysaccharides of <i>Brassica rapa</i> L.
The aim of this study was to investigate the effects of microwave ultrasonic-assisted extraction (MUAE) on the content, structure, and biological functions of <i>Brassica rapa</i> L. polysaccharide (BRP). Response surface methodology (RSM) was used to optimize the parameters of MUAE, and...
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MDPI AG
2022-05-01
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| author | Wenyang Cao Chenxi Wang Xiayidan Mayhesumu Le Pan Yan Dang Abulimiti Yili Aytursun Abuduwaili Sanawar Mansur |
| author_facet | Wenyang Cao Chenxi Wang Xiayidan Mayhesumu Le Pan Yan Dang Abulimiti Yili Aytursun Abuduwaili Sanawar Mansur |
| author_sort | Wenyang Cao |
| collection | DOAJ |
| description | The aim of this study was to investigate the effects of microwave ultrasonic-assisted extraction (MUAE) on the content, structure, and biological functions of <i>Brassica rapa</i> L. polysaccharide (BRP). Response surface methodology (RSM) was used to optimize the parameters of MUAE, and it obtained a polysaccharide with yield of 21.802%. Then, a neutral polysaccharide named BRP-1-1 with a molecular weight of 31.378 kDa was isolated and purified from BRP using DEAE-650 M and Sephadex G-100. The structures of the BRP-1-1 were elucidated through a combination of FT-IR, GC-MS, NMR, and methylation analysis. The results showed that BRP-1 consisted of mannose (Man) and glucose (Glu) in a molar ratio of 7.62:1. The backbone of BRP-1-1 mainly consisted of →6)-α-D-Glup-(1→4-β-D-Glup-(1→2)-α-D-Manp-(1→2)-α-D-Glup-(1→, the branch was [T-α-D-Manp-(1]<sub>n</sub>→. BRP-1-1 intervention significantly inhibited α-glucosidase activity; an inhibition rate of 44.623% was achieved at a concentration of 0.5 mg/mL. The results of the in vitro biological activity showed that BRP-1-1 has good antioxidant and hypoglycemic activity, suggesting that BRP-1-1 could be developed as a functional medicine. |
| first_indexed | 2024-03-10T03:53:11Z |
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| id | doaj.art-e93f9d6d192049259aa4ab466279c24e |
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| last_indexed | 2024-03-10T03:53:11Z |
| publishDate | 2022-05-01 |
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| series | Molecules |
| spelling | doaj.art-e93f9d6d192049259aa4ab466279c24e2023-11-23T08:52:46ZengMDPI AGMolecules1420-30492022-05-01279300210.3390/molecules27093002Isolation, Structural Elucidation, Antioxidant and Hypoglycemic Activity of Polysaccharides of <i>Brassica rapa</i> L.Wenyang Cao0Chenxi Wang1Xiayidan Mayhesumu2Le Pan3Yan Dang4Abulimiti Yili5Aytursun Abuduwaili6Sanawar Mansur7College of Chemistry and Chemical Engineering, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Chemistry and Chemical Engineering, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Chemistry and Chemical Engineering, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Chemistry and Chemical Engineering, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Chemistry and Chemical Engineering, Xinjiang Agricultural University, Urumqi 830052, ChinaKey Laboratory of Plants Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, ChinaCollege of Chemistry and Chemical Engineering, Xinjiang Agricultural University, Urumqi 830052, ChinaCollege of Chemistry and Chemical Engineering, Xinjiang Agricultural University, Urumqi 830052, ChinaThe aim of this study was to investigate the effects of microwave ultrasonic-assisted extraction (MUAE) on the content, structure, and biological functions of <i>Brassica rapa</i> L. polysaccharide (BRP). Response surface methodology (RSM) was used to optimize the parameters of MUAE, and it obtained a polysaccharide with yield of 21.802%. Then, a neutral polysaccharide named BRP-1-1 with a molecular weight of 31.378 kDa was isolated and purified from BRP using DEAE-650 M and Sephadex G-100. The structures of the BRP-1-1 were elucidated through a combination of FT-IR, GC-MS, NMR, and methylation analysis. The results showed that BRP-1 consisted of mannose (Man) and glucose (Glu) in a molar ratio of 7.62:1. The backbone of BRP-1-1 mainly consisted of →6)-α-D-Glup-(1→4-β-D-Glup-(1→2)-α-D-Manp-(1→2)-α-D-Glup-(1→, the branch was [T-α-D-Manp-(1]<sub>n</sub>→. BRP-1-1 intervention significantly inhibited α-glucosidase activity; an inhibition rate of 44.623% was achieved at a concentration of 0.5 mg/mL. The results of the in vitro biological activity showed that BRP-1-1 has good antioxidant and hypoglycemic activity, suggesting that BRP-1-1 could be developed as a functional medicine.https://www.mdpi.com/1420-3049/27/9/3002<i>Brassica rapa</i> L.MUAEpolysaccharidesantioxidant and hypoglycemic activitiesDPPHα-glucosidase |
| spellingShingle | Wenyang Cao Chenxi Wang Xiayidan Mayhesumu Le Pan Yan Dang Abulimiti Yili Aytursun Abuduwaili Sanawar Mansur Isolation, Structural Elucidation, Antioxidant and Hypoglycemic Activity of Polysaccharides of <i>Brassica rapa</i> L. Molecules <i>Brassica rapa</i> L. MUAE polysaccharides antioxidant and hypoglycemic activities DPPH α-glucosidase |
| title | Isolation, Structural Elucidation, Antioxidant and Hypoglycemic Activity of Polysaccharides of <i>Brassica rapa</i> L. |
| title_full | Isolation, Structural Elucidation, Antioxidant and Hypoglycemic Activity of Polysaccharides of <i>Brassica rapa</i> L. |
| title_fullStr | Isolation, Structural Elucidation, Antioxidant and Hypoglycemic Activity of Polysaccharides of <i>Brassica rapa</i> L. |
| title_full_unstemmed | Isolation, Structural Elucidation, Antioxidant and Hypoglycemic Activity of Polysaccharides of <i>Brassica rapa</i> L. |
| title_short | Isolation, Structural Elucidation, Antioxidant and Hypoglycemic Activity of Polysaccharides of <i>Brassica rapa</i> L. |
| title_sort | isolation structural elucidation antioxidant and hypoglycemic activity of polysaccharides of i brassica rapa i l |
| topic | <i>Brassica rapa</i> L. MUAE polysaccharides antioxidant and hypoglycemic activities DPPH α-glucosidase |
| url | https://www.mdpi.com/1420-3049/27/9/3002 |
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