Hsa_circRNA_0084043 promoting tumorigenesis in glioma through miR-577 sponging
Circular RNAs (circRNAs) are important non-coding RNAs (ncRNAs) involved in the development of multiple human diseases, especially cancers. circRNA_0084043 is significantly involved in the progression of melanoma. However, whether circRNA_0084043 is associated with glioma remains unknown. In this st...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-09-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023064277 |
| _version_ | 1797669936694296576 |
|---|---|
| author | Jin Qian Chunyan Chen Changping Zhao Xing Xu Yingna Xu Yong Zhang Chunfa Qian |
| author_facet | Jin Qian Chunyan Chen Changping Zhao Xing Xu Yingna Xu Yong Zhang Chunfa Qian |
| author_sort | Jin Qian |
| collection | DOAJ |
| description | Circular RNAs (circRNAs) are important non-coding RNAs (ncRNAs) involved in the development of multiple human diseases, especially cancers. circRNA_0084043 is significantly involved in the progression of melanoma. However, whether circRNA_0084043 is associated with glioma remains unknown. In this study, the upregulation of circRNA_0084043 in glioma and the association between circRNA_0084043 and glioma grade were identified. Our results showed that circRNA_0084043 is significantly involved in the proliferative, migratory, and invasive capacities of glioma cells. The results obtained from starBase, luciferase reporter assays, RNA immunoprecipitation assays, and RNA pull-down assays demonstrated that circRNA_0084043 acts as a direct sponge for miR-577. TargetScan algorithm was used to identify potential miR-577 targets, it was found that sorting nexin 5 (SNX5) is a candidate bound to miR-577. Finally, cell experiments testified that circRNA_0084043 enhanced growth, migration and invasion of glioma through the regulation of miR-577-mediated SNX5. Taken together, we concluded that circRNA_0084043 in the miR-577/SNX5 axis can be used as a candidate target for glioma therapy. |
| first_indexed | 2024-03-11T20:52:14Z |
| format | Article |
| id | doaj.art-e940ebb00cdc4a18b36cfe3b17db37d2 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-11T20:52:14Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-e940ebb00cdc4a18b36cfe3b17db37d22023-10-01T05:58:53ZengElsevierHeliyon2405-84402023-09-0199e19219Hsa_circRNA_0084043 promoting tumorigenesis in glioma through miR-577 spongingJin Qian0Chunyan Chen1Changping Zhao2Xing Xu3Yingna Xu4Yong Zhang5Chunfa Qian6Department of Neurosurgery, Xuancheng People's Hospital, The Affiliated Xuancheng Hospital of Wannan Medical College, Xuancheng, Anhui, China; Corresponding author. Department of Neurosurgery, Xuancheng People's Hospital, NO.51 Dabatang Road, 242099, Xuancheng, Anhui, China.Department of Gastroenterology and Hepatology, Jinling Hospital, Southern Medical University, Nanjing, ChinaDepartment of Neurosurgery, Xuancheng People's Hospital, The Affiliated Xuancheng Hospital of Wannan Medical College, Xuancheng, Anhui, ChinaDepartment of Neurosurgery, Xuancheng People's Hospital, The Affiliated Xuancheng Hospital of Wannan Medical College, Xuancheng, Anhui, ChinaDepartment of Neurosurgery, Xuancheng People's Hospital, The Affiliated Xuancheng Hospital of Wannan Medical College, Xuancheng, Anhui, ChinaDepartment of Neurosurgery, Xuancheng People's Hospital, The Affiliated Xuancheng Hospital of Wannan Medical College, Xuancheng, Anhui, ChinaDepartment of Neurosurgery, Affiliated Nanjing Brain Hospital, Nanjing Medical University, Nanjing, Jiangsu, China; Corresponding author. Department of Neurosurgery, Affiliated Nanjing Brain Hospital, Nanjing Medical University, NO.264 Guangzhou Road, 210029, Nanjing, Jiangsu, China.Circular RNAs (circRNAs) are important non-coding RNAs (ncRNAs) involved in the development of multiple human diseases, especially cancers. circRNA_0084043 is significantly involved in the progression of melanoma. However, whether circRNA_0084043 is associated with glioma remains unknown. In this study, the upregulation of circRNA_0084043 in glioma and the association between circRNA_0084043 and glioma grade were identified. Our results showed that circRNA_0084043 is significantly involved in the proliferative, migratory, and invasive capacities of glioma cells. The results obtained from starBase, luciferase reporter assays, RNA immunoprecipitation assays, and RNA pull-down assays demonstrated that circRNA_0084043 acts as a direct sponge for miR-577. TargetScan algorithm was used to identify potential miR-577 targets, it was found that sorting nexin 5 (SNX5) is a candidate bound to miR-577. Finally, cell experiments testified that circRNA_0084043 enhanced growth, migration and invasion of glioma through the regulation of miR-577-mediated SNX5. Taken together, we concluded that circRNA_0084043 in the miR-577/SNX5 axis can be used as a candidate target for glioma therapy.http://www.sciencedirect.com/science/article/pii/S2405844023064277GliomacircRNA_0084043miR-577SNX5 |
| spellingShingle | Jin Qian Chunyan Chen Changping Zhao Xing Xu Yingna Xu Yong Zhang Chunfa Qian Hsa_circRNA_0084043 promoting tumorigenesis in glioma through miR-577 sponging Heliyon Glioma circRNA_0084043 miR-577 SNX5 |
| title | Hsa_circRNA_0084043 promoting tumorigenesis in glioma through miR-577 sponging |
| title_full | Hsa_circRNA_0084043 promoting tumorigenesis in glioma through miR-577 sponging |
| title_fullStr | Hsa_circRNA_0084043 promoting tumorigenesis in glioma through miR-577 sponging |
| title_full_unstemmed | Hsa_circRNA_0084043 promoting tumorigenesis in glioma through miR-577 sponging |
| title_short | Hsa_circRNA_0084043 promoting tumorigenesis in glioma through miR-577 sponging |
| title_sort | hsa circrna 0084043 promoting tumorigenesis in glioma through mir 577 sponging |
| topic | Glioma circRNA_0084043 miR-577 SNX5 |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023064277 |
| work_keys_str_mv | AT jinqian hsacircrna0084043promotingtumorigenesisingliomathroughmir577sponging AT chunyanchen hsacircrna0084043promotingtumorigenesisingliomathroughmir577sponging AT changpingzhao hsacircrna0084043promotingtumorigenesisingliomathroughmir577sponging AT xingxu hsacircrna0084043promotingtumorigenesisingliomathroughmir577sponging AT yingnaxu hsacircrna0084043promotingtumorigenesisingliomathroughmir577sponging AT yongzhang hsacircrna0084043promotingtumorigenesisingliomathroughmir577sponging AT chunfaqian hsacircrna0084043promotingtumorigenesisingliomathroughmir577sponging |