Anti-angiogenic potential of novel 31kDa protein of Zanthoxylum rhesta is mediated by inhibition of HIF-1α nuclear translocation in vivo
A novel anticancer protein was isolated and purified from the pericarp of Zanthoxylum rhesta, a plant of culinary and ethnomedicinal importance from the Asian subcontinent. The partial purification process was followed by the fast protein liquid chromatography, gel permeation chromatography. This pu...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Advances in Cancer Biology - Metastasis |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667394022000375 |
| _version_ | 1818049600077955072 |
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| author | Priyanka Dattaraj Naik Parrikar K.S. Balaji K.K. Dharmappa A.D. Sathisha Shankar Jayarama |
| author_facet | Priyanka Dattaraj Naik Parrikar K.S. Balaji K.K. Dharmappa A.D. Sathisha Shankar Jayarama |
| author_sort | Priyanka Dattaraj Naik Parrikar |
| collection | DOAJ |
| description | A novel anticancer protein was isolated and purified from the pericarp of Zanthoxylum rhesta, a plant of culinary and ethnomedicinal importance from the Asian subcontinent. The partial purification process was followed by the fast protein liquid chromatography, gel permeation chromatography. This purified protein was obtained as a single band in the SDS-PAGE with molecular mass of 31 kDa and was named as ZRP. It showed anti-angiogenic activity in vivo in Swiss albino mice inoculated with EAC cells indicated by the reduced peritoneal angiogenesis. In ovo CAM assay also showed reduced neoangiogenesis. ZRP accompanied in VEGF reduction due to hindered nuclear translocation of HIF-1α which is a clear evidence of anti-angiogenic potential of the ZRP protein. |
| first_indexed | 2024-12-10T10:40:09Z |
| format | Article |
| id | doaj.art-e9433644b51f45c7a87a9e1e0b2b892a |
| institution | Directory Open Access Journal |
| issn | 2667-3940 |
| language | English |
| last_indexed | 2024-12-10T10:40:09Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Advances in Cancer Biology - Metastasis |
| spelling | doaj.art-e9433644b51f45c7a87a9e1e0b2b892a2022-12-22T01:52:20ZengElsevierAdvances in Cancer Biology - Metastasis2667-39402022-10-015100063Anti-angiogenic potential of novel 31kDa protein of Zanthoxylum rhesta is mediated by inhibition of HIF-1α nuclear translocation in vivoPriyanka Dattaraj Naik Parrikar0K.S. Balaji1K.K. Dharmappa2A.D. Sathisha3Shankar Jayarama4Department of Biotechnology, DCT's Dhempe College of Arts and Science, Miramar, Goa, 403501, India; Post Graduate Department of Biotechnology, Teresian College, Siddharthanagar, Mysore, IndiaDepartment of Physiology, Eastern Virginia Medical School, Norfolk, VA, USADepartment of Biochemistry, Chika Aluvara PG Center, Mangalore University, Mangalore, Karnataka, IndiaDepartment of Medical Biochemistry and Molecular Biology, College of Health Sciences, Mekelle University, EthiopiaDepartment of Studies in Food Technology, Davengere University, Davengere, Karnataka, 57700, India; Corresponding author.A novel anticancer protein was isolated and purified from the pericarp of Zanthoxylum rhesta, a plant of culinary and ethnomedicinal importance from the Asian subcontinent. The partial purification process was followed by the fast protein liquid chromatography, gel permeation chromatography. This purified protein was obtained as a single band in the SDS-PAGE with molecular mass of 31 kDa and was named as ZRP. It showed anti-angiogenic activity in vivo in Swiss albino mice inoculated with EAC cells indicated by the reduced peritoneal angiogenesis. In ovo CAM assay also showed reduced neoangiogenesis. ZRP accompanied in VEGF reduction due to hindered nuclear translocation of HIF-1α which is a clear evidence of anti-angiogenic potential of the ZRP protein.http://www.sciencedirect.com/science/article/pii/S2667394022000375Anti-angiogenicProteinZ. rhestaEthnomedicinalVEGFEAC |
| spellingShingle | Priyanka Dattaraj Naik Parrikar K.S. Balaji K.K. Dharmappa A.D. Sathisha Shankar Jayarama Anti-angiogenic potential of novel 31kDa protein of Zanthoxylum rhesta is mediated by inhibition of HIF-1α nuclear translocation in vivo Advances in Cancer Biology - Metastasis Anti-angiogenic Protein Z. rhesta Ethnomedicinal VEGF EAC |
| title | Anti-angiogenic potential of novel 31kDa protein of Zanthoxylum rhesta is mediated by inhibition of HIF-1α nuclear translocation in vivo |
| title_full | Anti-angiogenic potential of novel 31kDa protein of Zanthoxylum rhesta is mediated by inhibition of HIF-1α nuclear translocation in vivo |
| title_fullStr | Anti-angiogenic potential of novel 31kDa protein of Zanthoxylum rhesta is mediated by inhibition of HIF-1α nuclear translocation in vivo |
| title_full_unstemmed | Anti-angiogenic potential of novel 31kDa protein of Zanthoxylum rhesta is mediated by inhibition of HIF-1α nuclear translocation in vivo |
| title_short | Anti-angiogenic potential of novel 31kDa protein of Zanthoxylum rhesta is mediated by inhibition of HIF-1α nuclear translocation in vivo |
| title_sort | anti angiogenic potential of novel 31kda protein of zanthoxylum rhesta is mediated by inhibition of hif 1α nuclear translocation in vivo |
| topic | Anti-angiogenic Protein Z. rhesta Ethnomedicinal VEGF EAC |
| url | http://www.sciencedirect.com/science/article/pii/S2667394022000375 |
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