Methylation and polymorphism in CDH1 gene promoter among patients with diffuse gastric cancer
Background: The promoter methylation and single nucleotide polymorphisms (SNPs) affect the transcription activity of cancer-related genes in several cancers including diffuse gastric cancer (DGC). Here we aimed to evaluate the promoter methylation status and the rs16260 at the promoter region of the...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | International Journal of Preventive Medicine |
| Subjects: | |
| Online Access: | http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2022;volume=13;issue=1;spage=44;epage=44;aulast=Vishteh |
| _version_ | 1819062087922483200 |
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| author | Mohadeseh Naghi Vishteh Mehrdad Zeinalian Majid Kheirollahi Amirreza Javadi Mamaghani Mohammad Ali Zolfaghari Aliyar Mirzapour Meisam Barati Seyed Javad Seyed Tabaei |
| author_facet | Mohadeseh Naghi Vishteh Mehrdad Zeinalian Majid Kheirollahi Amirreza Javadi Mamaghani Mohammad Ali Zolfaghari Aliyar Mirzapour Meisam Barati Seyed Javad Seyed Tabaei |
| author_sort | Mohadeseh Naghi Vishteh |
| collection | DOAJ |
| description | Background: The promoter methylation and single nucleotide polymorphisms (SNPs) affect the transcription activity of cancer-related genes in several cancers including diffuse gastric cancer (DGC). Here we aimed to evaluate the promoter methylation status and the rs16260 at the promoter region of the CDH1 gene in DGC. Methods: This case-control study was performed of 48 formalin-fixed paraffin-embedded (FFPE) blocks of DGC patients and 41 fresh frozen tissue samples of healthy individuals. Methylation status was evaluated using methylation-specific polymerase chain reaction (PCR) and the rs16260 at the promoter region of the CDH1 gene was assessed using PCR and sequencing method. Results: The occurrence of methylation at the promoter region of the CDH1 gene in DGC patients was significantly higher than control samples (P < 0.0001). The methylated status was significantly associated with the poor differentiated histological type of DGC (P = 0.0428). The frequency of AC genotype and the A allele in DGC patients was significantly higher than the control subjects (P = 0.006 and 0.003, respectively). Conclusions: Here we showed that methylation at the CDH1 promoter may contribute to the DGC development, and also the AC genotype was associated with the risk of DGC. |
| first_indexed | 2024-12-21T14:53:13Z |
| format | Article |
| id | doaj.art-e945d18d26524b6193723d77f8030c47 |
| institution | Directory Open Access Journal |
| issn | 2008-7802 2008-8213 |
| language | English |
| last_indexed | 2024-12-21T14:53:13Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | International Journal of Preventive Medicine |
| spelling | doaj.art-e945d18d26524b6193723d77f8030c472022-12-21T18:59:49ZengWolters Kluwer Medknow PublicationsInternational Journal of Preventive Medicine2008-78022008-82132022-01-01131444410.4103/ijpvm.IJPVM_288_20Methylation and polymorphism in CDH1 gene promoter among patients with diffuse gastric cancerMohadeseh Naghi VishtehMehrdad ZeinalianMajid KheirollahiAmirreza Javadi MamaghaniMohammad Ali ZolfaghariAliyar MirzapourMeisam BaratiSeyed Javad Seyed TabaeiBackground: The promoter methylation and single nucleotide polymorphisms (SNPs) affect the transcription activity of cancer-related genes in several cancers including diffuse gastric cancer (DGC). Here we aimed to evaluate the promoter methylation status and the rs16260 at the promoter region of the CDH1 gene in DGC. Methods: This case-control study was performed of 48 formalin-fixed paraffin-embedded (FFPE) blocks of DGC patients and 41 fresh frozen tissue samples of healthy individuals. Methylation status was evaluated using methylation-specific polymerase chain reaction (PCR) and the rs16260 at the promoter region of the CDH1 gene was assessed using PCR and sequencing method. Results: The occurrence of methylation at the promoter region of the CDH1 gene in DGC patients was significantly higher than control samples (P < 0.0001). The methylated status was significantly associated with the poor differentiated histological type of DGC (P = 0.0428). The frequency of AC genotype and the A allele in DGC patients was significantly higher than the control subjects (P = 0.006 and 0.003, respectively). Conclusions: Here we showed that methylation at the CDH1 promoter may contribute to the DGC development, and also the AC genotype was associated with the risk of DGC.http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2022;volume=13;issue=1;spage=44;epage=44;aulast=Vishtehcadherin 1methylationpolymerase chain reactionsequence analysisstomach neoplasms |
| spellingShingle | Mohadeseh Naghi Vishteh Mehrdad Zeinalian Majid Kheirollahi Amirreza Javadi Mamaghani Mohammad Ali Zolfaghari Aliyar Mirzapour Meisam Barati Seyed Javad Seyed Tabaei Methylation and polymorphism in CDH1 gene promoter among patients with diffuse gastric cancer International Journal of Preventive Medicine cadherin 1 methylation polymerase chain reaction sequence analysis stomach neoplasms |
| title | Methylation and polymorphism in CDH1 gene promoter among patients with diffuse gastric cancer |
| title_full | Methylation and polymorphism in CDH1 gene promoter among patients with diffuse gastric cancer |
| title_fullStr | Methylation and polymorphism in CDH1 gene promoter among patients with diffuse gastric cancer |
| title_full_unstemmed | Methylation and polymorphism in CDH1 gene promoter among patients with diffuse gastric cancer |
| title_short | Methylation and polymorphism in CDH1 gene promoter among patients with diffuse gastric cancer |
| title_sort | methylation and polymorphism in cdh1 gene promoter among patients with diffuse gastric cancer |
| topic | cadherin 1 methylation polymerase chain reaction sequence analysis stomach neoplasms |
| url | http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2022;volume=13;issue=1;spage=44;epage=44;aulast=Vishteh |
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