Electroacupuncture at ST36 modulates the intestinal microecology and may help repair the intestinal barrier in the rat model of severe acute pancreatitis
Abstract Severe acute pancreatitis (SAP) onset and development are closely associated with intestinal barrier injury. Evidence from clinical practice and research has shown that electroacupuncture (EA) at the Zusanli (ST36) acupoint can improve intestinal barrier function and abdominal symptoms in p...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-02-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.14401 |
| _version_ | 1797294808346132480 |
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| author | Huimin Xu Qian Wen Hangqi Hu Sihao Yang Lingyun Lu Xiangyun Hu Hao Li Xianhao Huang Ning Li |
| author_facet | Huimin Xu Qian Wen Hangqi Hu Sihao Yang Lingyun Lu Xiangyun Hu Hao Li Xianhao Huang Ning Li |
| author_sort | Huimin Xu |
| collection | DOAJ |
| description | Abstract Severe acute pancreatitis (SAP) onset and development are closely associated with intestinal barrier injury. Evidence from clinical practice and research has shown that electroacupuncture (EA) at the Zusanli (ST36) acupoint can improve intestinal barrier function and abdominal symptoms in patients with SAP; however, the specific mechanisms of action remain unclear. This study aimed to observe the changes in the intestinal microbiota and metabolites in SAP rats and to explore the effect of EA at ST36 on intestinal barrier injury in SAP rats. 16S rRNA gene sequencing combined with microbial diversity analysis, short‐chain fatty acids (SCFAs)‐targeted metabolomics, immunohistochemistry, immunofluorescence, western blotting, and other techniques were used to explore the mechanism of EA at bilateral ST36 acupoints on SAP‐related intestinal barrier injury. Our results showed that EA at ST36 could repair intestinal barrier injury by modulating intestinal microecology, thereby reducing intestinal inflammation, restoring intestinal function, and ultimately alleviating the prognosis of SAP. Our study provides new insights into the mechanisms and treatment of intestinal barrier injury in patients with SAP from the perspectives of microbiota and SCFAs regulation. |
| first_indexed | 2024-03-07T21:37:04Z |
| format | Article |
| id | doaj.art-e945e1b06d364091b69d0900504116fb |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-03-07T21:37:04Z |
| publishDate | 2024-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-e945e1b06d364091b69d0900504116fb2024-02-26T12:12:42ZengWileyMicrobial Biotechnology1751-79152024-02-01172n/an/a10.1111/1751-7915.14401Electroacupuncture at ST36 modulates the intestinal microecology and may help repair the intestinal barrier in the rat model of severe acute pancreatitisHuimin Xu0Qian Wen1Hangqi Hu2Sihao Yang3Lingyun Lu4Xiangyun Hu5Hao Li6Xianhao Huang7Ning Li8Institute of Integrated Traditional Chinese and Western Medicine West China Hospital, Sichuan University Chengdu Sichuan ChinaInstitute of Integrated Traditional Chinese and Western Medicine West China Hospital, Sichuan University Chengdu Sichuan ChinaInstitute of Integrated Traditional Chinese and Western Medicine West China Hospital, Sichuan University Chengdu Sichuan ChinaDepartment of Integrated Traditional Chinese and Western Medicine Yibin Second People's Hospital Yibin Sichuan ChinaInstitute of Integrated Traditional Chinese and Western Medicine West China Hospital, Sichuan University Chengdu Sichuan ChinaChengdu University of Traditional Chinese Medicine Chengdu Sichuan ChinaInstitute of Integrated Traditional Chinese and Western Medicine West China Hospital, Sichuan University Chengdu Sichuan ChinaInstitute of Integrated Traditional Chinese and Western Medicine West China Hospital, Sichuan University Chengdu Sichuan ChinaInstitute of Integrated Traditional Chinese and Western Medicine West China Hospital, Sichuan University Chengdu Sichuan ChinaAbstract Severe acute pancreatitis (SAP) onset and development are closely associated with intestinal barrier injury. Evidence from clinical practice and research has shown that electroacupuncture (EA) at the Zusanli (ST36) acupoint can improve intestinal barrier function and abdominal symptoms in patients with SAP; however, the specific mechanisms of action remain unclear. This study aimed to observe the changes in the intestinal microbiota and metabolites in SAP rats and to explore the effect of EA at ST36 on intestinal barrier injury in SAP rats. 16S rRNA gene sequencing combined with microbial diversity analysis, short‐chain fatty acids (SCFAs)‐targeted metabolomics, immunohistochemistry, immunofluorescence, western blotting, and other techniques were used to explore the mechanism of EA at bilateral ST36 acupoints on SAP‐related intestinal barrier injury. Our results showed that EA at ST36 could repair intestinal barrier injury by modulating intestinal microecology, thereby reducing intestinal inflammation, restoring intestinal function, and ultimately alleviating the prognosis of SAP. Our study provides new insights into the mechanisms and treatment of intestinal barrier injury in patients with SAP from the perspectives of microbiota and SCFAs regulation.https://doi.org/10.1111/1751-7915.14401 |
| spellingShingle | Huimin Xu Qian Wen Hangqi Hu Sihao Yang Lingyun Lu Xiangyun Hu Hao Li Xianhao Huang Ning Li Electroacupuncture at ST36 modulates the intestinal microecology and may help repair the intestinal barrier in the rat model of severe acute pancreatitis Microbial Biotechnology |
| title | Electroacupuncture at ST36 modulates the intestinal microecology and may help repair the intestinal barrier in the rat model of severe acute pancreatitis |
| title_full | Electroacupuncture at ST36 modulates the intestinal microecology and may help repair the intestinal barrier in the rat model of severe acute pancreatitis |
| title_fullStr | Electroacupuncture at ST36 modulates the intestinal microecology and may help repair the intestinal barrier in the rat model of severe acute pancreatitis |
| title_full_unstemmed | Electroacupuncture at ST36 modulates the intestinal microecology and may help repair the intestinal barrier in the rat model of severe acute pancreatitis |
| title_short | Electroacupuncture at ST36 modulates the intestinal microecology and may help repair the intestinal barrier in the rat model of severe acute pancreatitis |
| title_sort | electroacupuncture at st36 modulates the intestinal microecology and may help repair the intestinal barrier in the rat model of severe acute pancreatitis |
| url | https://doi.org/10.1111/1751-7915.14401 |
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