Generation of induced pluripotent stem cell line (IPTi001-A) from a 62-year old sporadic Alzheimer's disease patient with APOE3 (ε3/ε3) genotype
A 62-year old sporadic Alzheimer's disease (sAD) male patient with APOE3 (ε3/ε3) genotype donated his peripheral blood mononuclear cells (PBMCs). The induced pluripotent stem cell (iPSC) line was established by episomal vector system with the four factors OCT4, SOX2, C-MYC and KLF4. EB differen...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-12-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506119302193 |
| _version_ | 1818024390170771456 |
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| author | Lin Zhang Mei Xu Gang Liu Rongrong Wu Shulin Meng Kang Xiahou Ning Jiang Yongxiang Zhang Wenxia Zhou |
| author_facet | Lin Zhang Mei Xu Gang Liu Rongrong Wu Shulin Meng Kang Xiahou Ning Jiang Yongxiang Zhang Wenxia Zhou |
| author_sort | Lin Zhang |
| collection | DOAJ |
| description | A 62-year old sporadic Alzheimer's disease (sAD) male patient with APOE3 (ε3/ε3) genotype donated his peripheral blood mononuclear cells (PBMCs). The induced pluripotent stem cell (iPSC) line was established by episomal vector system with the four factors OCT4, SOX2, C-MYC and KLF4. EB differentiation in vitro showed that iPSC line had a potential to differentiate into three germ layers. More than 90% cells expressed NANOG, OCT4 and SSEA4 detected by flow cytometry. In addition, the iPSC line was karyotypically normal. The iPSC line may provide new valuable tools for studying pathogenesis of sAD and screening candidate drugs for the disease. |
| first_indexed | 2024-12-10T03:59:27Z |
| format | Article |
| id | doaj.art-e94688660045465f9a32e3ecd6ca865b |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-10T03:59:27Z |
| publishDate | 2019-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-e94688660045465f9a32e3ecd6ca865b2022-12-22T02:03:01ZengElsevierStem Cell Research1873-50612019-12-0141Generation of induced pluripotent stem cell line (IPTi001-A) from a 62-year old sporadic Alzheimer's disease patient with APOE3 (ε3/ε3) genotypeLin Zhang0Mei Xu1Gang Liu2Rongrong Wu3Shulin Meng4Kang Xiahou5Ning Jiang6Yongxiang Zhang7Wenxia Zhou8Beijing Institute of Pharmacology and Toxicology, Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China; School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, ChinaBeijing Institute of Pharmacology and Toxicology, Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, ChinaBeijing Institute of Pharmacology and Toxicology, Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, ChinaBeijing 302 Hospital, Beijing, ChinaIxCell BIOTECHNOLOGY Co.,Ltd, Shanghai, ChinaIxCell BIOTECHNOLOGY Co.,Ltd, Shanghai, ChinaBeijing Institute of Pharmacology and Toxicology, Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China; Correspondence authors at: Beijing Institute of Pharmacology and Toxicology; 27 Taiping Road, 100850, Beijing, China.Beijing Institute of Pharmacology and Toxicology, Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China; School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, China; Correspondence authors at: Beijing Institute of Pharmacology and Toxicology; 27 Taiping Road, 100850, Beijing, China.Beijing Institute of Pharmacology and Toxicology, Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China; Correspondence authors at: Beijing Institute of Pharmacology and Toxicology; 27 Taiping Road, 100850, Beijing, China.A 62-year old sporadic Alzheimer's disease (sAD) male patient with APOE3 (ε3/ε3) genotype donated his peripheral blood mononuclear cells (PBMCs). The induced pluripotent stem cell (iPSC) line was established by episomal vector system with the four factors OCT4, SOX2, C-MYC and KLF4. EB differentiation in vitro showed that iPSC line had a potential to differentiate into three germ layers. More than 90% cells expressed NANOG, OCT4 and SSEA4 detected by flow cytometry. In addition, the iPSC line was karyotypically normal. The iPSC line may provide new valuable tools for studying pathogenesis of sAD and screening candidate drugs for the disease.http://www.sciencedirect.com/science/article/pii/S1873506119302193 |
| spellingShingle | Lin Zhang Mei Xu Gang Liu Rongrong Wu Shulin Meng Kang Xiahou Ning Jiang Yongxiang Zhang Wenxia Zhou Generation of induced pluripotent stem cell line (IPTi001-A) from a 62-year old sporadic Alzheimer's disease patient with APOE3 (ε3/ε3) genotype Stem Cell Research |
| title | Generation of induced pluripotent stem cell line (IPTi001-A) from a 62-year old sporadic Alzheimer's disease patient with APOE3 (ε3/ε3) genotype |
| title_full | Generation of induced pluripotent stem cell line (IPTi001-A) from a 62-year old sporadic Alzheimer's disease patient with APOE3 (ε3/ε3) genotype |
| title_fullStr | Generation of induced pluripotent stem cell line (IPTi001-A) from a 62-year old sporadic Alzheimer's disease patient with APOE3 (ε3/ε3) genotype |
| title_full_unstemmed | Generation of induced pluripotent stem cell line (IPTi001-A) from a 62-year old sporadic Alzheimer's disease patient with APOE3 (ε3/ε3) genotype |
| title_short | Generation of induced pluripotent stem cell line (IPTi001-A) from a 62-year old sporadic Alzheimer's disease patient with APOE3 (ε3/ε3) genotype |
| title_sort | generation of induced pluripotent stem cell line ipti001 a from a 62 year old sporadic alzheimer s disease patient with apoe3 ε3 ε3 genotype |
| url | http://www.sciencedirect.com/science/article/pii/S1873506119302193 |
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