The value of biofilm testing to guide antimicrobial stewardship in chronic respiratory diseases
IntroductionBiofilm production is an important yet currently overlooked aspect of diagnostic microbiology that has implications for antimicrobial stewardship. In this study, we aimed to validate and identify additional applications of the BioFilm Ring Test® (BRT) for Pseudomonas aeruginosa (PA) isol...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1142274/full |
| _version_ | 1797835063973380096 |
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| author | Laia Fernández-Barat Laia Fernández-Barat Nil Vázquez Burgos Nil Vázquez Burgos Victoria Alcaraz Victoria Alcaraz Leticia Bueno-Freire Leticia Bueno-Freire Ruben López-Aladid Ruben López-Aladid Roberto Cabrera Roberto Cabrera Albert Gabarrús Albert Gabarrús Andrea Palomeque Andrea Palomeque Patricia Oscanoa Patricia Oscanoa Adrian Ceccato Adrian Ceccato Ana Motos Ana Motos Rosanel Amaro Rosanel Amaro Thierry Bernardi Thierry Bernardi Christian Provot Christian Provot Alba Soler-Comas Alba Soler-Comas Laura Muñoz Jordi Vila Antoni Torres Antoni Torres |
| author_facet | Laia Fernández-Barat Laia Fernández-Barat Nil Vázquez Burgos Nil Vázquez Burgos Victoria Alcaraz Victoria Alcaraz Leticia Bueno-Freire Leticia Bueno-Freire Ruben López-Aladid Ruben López-Aladid Roberto Cabrera Roberto Cabrera Albert Gabarrús Albert Gabarrús Andrea Palomeque Andrea Palomeque Patricia Oscanoa Patricia Oscanoa Adrian Ceccato Adrian Ceccato Ana Motos Ana Motos Rosanel Amaro Rosanel Amaro Thierry Bernardi Thierry Bernardi Christian Provot Christian Provot Alba Soler-Comas Alba Soler-Comas Laura Muñoz Jordi Vila Antoni Torres Antoni Torres |
| author_sort | Laia Fernández-Barat |
| collection | DOAJ |
| description | IntroductionBiofilm production is an important yet currently overlooked aspect of diagnostic microbiology that has implications for antimicrobial stewardship. In this study, we aimed to validate and identify additional applications of the BioFilm Ring Test® (BRT) for Pseudomonas aeruginosa (PA) isolates from patients with bronchiectasis (BE).Materials and methodsSputa were collected from BE patients who had at least one PA positive culture in the previous year. We processed the sputa to isolate both mucoid and non-mucoid PA, and determined their susceptibility pattern, mucA gene status, and presence of ciprofloxacin mutations in QRDR genes. The Biofilm production index (BPI) was obtained at 5 and 24 hours. Biofilms were imaged using Gram staining.ResultsWe collected 69 PA isolates, including 33 mucoid and 36 non-mucoid. A BPI value below 14.75 at 5 hours predicted the mucoid PA phenotype with 64% sensitivity and 72% specificity.ConclusionOverall, our findings suggest that the fitness-cost associated with the mucoid phenotype or ciprofloxacin resistance is shown through a time-dependent BPI profile. The BRT has the potential to reveal biofilm features with clinical implications. |
| first_indexed | 2024-04-09T14:47:13Z |
| format | Article |
| id | doaj.art-e94722d381f84baaa509c3f5521789fc |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-09T14:47:13Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-e94722d381f84baaa509c3f5521789fc2023-05-02T13:32:26ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-05-011310.3389/fcimb.2023.11422741142274The value of biofilm testing to guide antimicrobial stewardship in chronic respiratory diseasesLaia Fernández-Barat0Laia Fernández-Barat1Nil Vázquez Burgos2Nil Vázquez Burgos3Victoria Alcaraz4Victoria Alcaraz5Leticia Bueno-Freire6Leticia Bueno-Freire7Ruben López-Aladid8Ruben López-Aladid9Roberto Cabrera10Roberto Cabrera11Albert Gabarrús12Albert Gabarrús13Andrea Palomeque14Andrea Palomeque15Patricia Oscanoa16Patricia Oscanoa17Adrian Ceccato18Adrian Ceccato19Ana Motos20Ana Motos21Rosanel Amaro22Rosanel Amaro23Thierry Bernardi24Thierry Bernardi25Christian Provot26Christian Provot27Alba Soler-Comas28Alba Soler-Comas29Laura Muñoz30Jordi Vila31Antoni Torres32Antoni Torres33Cellex Laboratory, CibeRes (CB06/06/0028)-Instituto de Investigaciones Biomédicas August Pi i Sunyer (IDIBAPS), School of Medicine, University of Barcelona, Barcelona, SpainPneumology Service, Respiratory Institute, Hospital Clinic, Barcelona, SpainCellex Laboratory, CibeRes (CB06/06/0028)-Instituto de Investigaciones Biomédicas August Pi i Sunyer (IDIBAPS), School of Medicine, University of Barcelona, Barcelona, SpainPneumology Service, Respiratory Institute, Hospital Clinic, Barcelona, SpainCellex Laboratory, CibeRes (CB06/06/0028)-Instituto de Investigaciones Biomédicas August Pi i Sunyer (IDIBAPS), School of Medicine, University of Barcelona, Barcelona, SpainPneumology Service, Respiratory Institute, Hospital Clinic, Barcelona, SpainCellex Laboratory, CibeRes (CB06/06/0028)-Instituto de Investigaciones Biomédicas August Pi i Sunyer (IDIBAPS), School of Medicine, University of Barcelona, Barcelona, SpainPneumology Service, Respiratory Institute, Hospital Clinic, Barcelona, SpainCellex Laboratory, CibeRes (CB06/06/0028)-Instituto de Investigaciones Biomédicas August Pi i Sunyer (IDIBAPS), School of Medicine, University of Barcelona, Barcelona, SpainPneumology Service, Respiratory Institute, Hospital Clinic, Barcelona, SpainCellex Laboratory, CibeRes (CB06/06/0028)-Instituto de Investigaciones Biomédicas August Pi i Sunyer (IDIBAPS), School of Medicine, University of Barcelona, Barcelona, SpainPneumology Service, Respiratory Institute, Hospital Clinic, Barcelona, SpainCellex Laboratory, CibeRes (CB06/06/0028)-Instituto de Investigaciones Biomédicas August Pi i Sunyer (IDIBAPS), School of Medicine, University of Barcelona, Barcelona, SpainPneumology Service, Respiratory Institute, Hospital Clinic, Barcelona, SpainCellex Laboratory, CibeRes (CB06/06/0028)-Instituto de Investigaciones Biomédicas August Pi i Sunyer (IDIBAPS), School of Medicine, University of Barcelona, Barcelona, SpainPneumology Service, Respiratory Institute, Hospital Clinic, Barcelona, SpainCellex Laboratory, CibeRes (CB06/06/0028)-Instituto de Investigaciones Biomédicas August Pi i Sunyer (IDIBAPS), School of Medicine, University of Barcelona, Barcelona, SpainPneumology Service, Respiratory Institute, Hospital Clinic, Barcelona, SpainCellex Laboratory, CibeRes (CB06/06/0028)-Instituto de Investigaciones Biomédicas August Pi i Sunyer (IDIBAPS), School of Medicine, University of Barcelona, Barcelona, SpainPneumology Service, Respiratory Institute, Hospital Clinic, Barcelona, SpainCellex Laboratory, CibeRes (CB06/06/0028)-Instituto de Investigaciones Biomédicas August Pi i Sunyer (IDIBAPS), School of Medicine, University of Barcelona, Barcelona, SpainPneumology Service, Respiratory Institute, Hospital Clinic, Barcelona, SpainCellex Laboratory, CibeRes (CB06/06/0028)-Instituto de Investigaciones Biomédicas August Pi i Sunyer (IDIBAPS), School of Medicine, University of Barcelona, Barcelona, SpainPneumology Service, Respiratory Institute, Hospital Clinic, Barcelona, SpainBioFilm Pharma SAS, Lyon, FranceBioFilm Control SAS, Saint Beauzire, FranceBioFilm Pharma SAS, Lyon, FranceBioFilm Control SAS, Saint Beauzire, FranceCellex Laboratory, CibeRes (CB06/06/0028)-Instituto de Investigaciones Biomédicas August Pi i Sunyer (IDIBAPS), School of Medicine, University of Barcelona, Barcelona, SpainPneumology Service, Respiratory Institute, Hospital Clinic, Barcelona, SpainMicrobiology Service, Hospital Clinic, Barcelona, SpainMicrobiology Service, Hospital Clinic, Barcelona, SpainCellex Laboratory, CibeRes (CB06/06/0028)-Instituto de Investigaciones Biomédicas August Pi i Sunyer (IDIBAPS), School of Medicine, University of Barcelona, Barcelona, SpainPneumology Service, Respiratory Institute, Hospital Clinic, Barcelona, SpainIntroductionBiofilm production is an important yet currently overlooked aspect of diagnostic microbiology that has implications for antimicrobial stewardship. In this study, we aimed to validate and identify additional applications of the BioFilm Ring Test® (BRT) for Pseudomonas aeruginosa (PA) isolates from patients with bronchiectasis (BE).Materials and methodsSputa were collected from BE patients who had at least one PA positive culture in the previous year. We processed the sputa to isolate both mucoid and non-mucoid PA, and determined their susceptibility pattern, mucA gene status, and presence of ciprofloxacin mutations in QRDR genes. The Biofilm production index (BPI) was obtained at 5 and 24 hours. Biofilms were imaged using Gram staining.ResultsWe collected 69 PA isolates, including 33 mucoid and 36 non-mucoid. A BPI value below 14.75 at 5 hours predicted the mucoid PA phenotype with 64% sensitivity and 72% specificity.ConclusionOverall, our findings suggest that the fitness-cost associated with the mucoid phenotype or ciprofloxacin resistance is shown through a time-dependent BPI profile. The BRT has the potential to reveal biofilm features with clinical implications.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1142274/fullPseudomonas aeruginosaantimicrobial agentsantimicrobial resistancesbiofilm diagnosebiofilm |
| spellingShingle | Laia Fernández-Barat Laia Fernández-Barat Nil Vázquez Burgos Nil Vázquez Burgos Victoria Alcaraz Victoria Alcaraz Leticia Bueno-Freire Leticia Bueno-Freire Ruben López-Aladid Ruben López-Aladid Roberto Cabrera Roberto Cabrera Albert Gabarrús Albert Gabarrús Andrea Palomeque Andrea Palomeque Patricia Oscanoa Patricia Oscanoa Adrian Ceccato Adrian Ceccato Ana Motos Ana Motos Rosanel Amaro Rosanel Amaro Thierry Bernardi Thierry Bernardi Christian Provot Christian Provot Alba Soler-Comas Alba Soler-Comas Laura Muñoz Jordi Vila Antoni Torres Antoni Torres The value of biofilm testing to guide antimicrobial stewardship in chronic respiratory diseases Frontiers in Cellular and Infection Microbiology Pseudomonas aeruginosa antimicrobial agents antimicrobial resistances biofilm diagnose biofilm |
| title | The value of biofilm testing to guide antimicrobial stewardship in chronic respiratory diseases |
| title_full | The value of biofilm testing to guide antimicrobial stewardship in chronic respiratory diseases |
| title_fullStr | The value of biofilm testing to guide antimicrobial stewardship in chronic respiratory diseases |
| title_full_unstemmed | The value of biofilm testing to guide antimicrobial stewardship in chronic respiratory diseases |
| title_short | The value of biofilm testing to guide antimicrobial stewardship in chronic respiratory diseases |
| title_sort | value of biofilm testing to guide antimicrobial stewardship in chronic respiratory diseases |
| topic | Pseudomonas aeruginosa antimicrobial agents antimicrobial resistances biofilm diagnose biofilm |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1142274/full |
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