Differential Proteomic Analysis of <em>Listeria monocytogenes</em> during High-Pressure Processing
High-pressure processing (HPP) is a prevailing non-thermal food preservation technology. The inactivation mechanisms of <i>Listeria monocytogenes</i> under HPP at 200 and 400 MPa for 3 min were investigated by label-free quantitative proteomic analysis and functional enrichment analysis...
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MDPI AG
2022-07-01
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| Online Access: | https://www.mdpi.com/2079-7737/11/8/1152 |
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| author | Yi-An Chen Guan-Wen Chen Hao-Hsiang Ku Tsui-Chin Huang Hsin-Yi Chang Cheng-I Wei Yung-Hsiang Tsai Tai-Yuan Chen |
| author_facet | Yi-An Chen Guan-Wen Chen Hao-Hsiang Ku Tsui-Chin Huang Hsin-Yi Chang Cheng-I Wei Yung-Hsiang Tsai Tai-Yuan Chen |
| author_sort | Yi-An Chen |
| collection | DOAJ |
| description | High-pressure processing (HPP) is a prevailing non-thermal food preservation technology. The inactivation mechanisms of <i>Listeria monocytogenes</i> under HPP at 200 and 400 MPa for 3 min were investigated by label-free quantitative proteomic analysis and functional enrichment analysis in the Kyoto Encyclopedia of Genes and Genomes. HPP treatment at 400 MPa exhibited significant effects on proteins involved in translation, carbon, carbohydrate, lipid and energy metabolism, and peptidoglycan biosynthesis. HPP increased most ribosomal subunits and initiation factors, suggesting it might shift ribosomal biogenesis to translation initiation. However, protein synthesis was impaired by the shortage of proteins responsible for elongation, termination and recycling. HPP stimulated several ATP-dependent Clp proteases, and the global transcriptional regulator Spx, associating with activation of the stress-activated sigma factor Sigma B (σ<sup>B</sup>) and the transcriptional activator positive regulatory factor A (PrfA) regulons. The quantitative proteomics approaches provide fundamental information on <i>L. monocytogenes</i> under different HPP pressures, and provide theoretical support for HPP against Listeriosis illness and for promotion of safer ready-to-eat foods. |
| first_indexed | 2024-03-09T10:02:01Z |
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| id | doaj.art-e94817ce0cd44ec39b0c58ee0d8075a3 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T10:02:01Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-e94817ce0cd44ec39b0c58ee0d8075a32023-12-01T23:26:06ZengMDPI AGBiology2079-77372022-07-01118115210.3390/biology11081152Differential Proteomic Analysis of <em>Listeria monocytogenes</em> during High-Pressure ProcessingYi-An Chen0Guan-Wen Chen1Hao-Hsiang Ku2Tsui-Chin Huang3Hsin-Yi Chang4Cheng-I Wei5Yung-Hsiang Tsai6Tai-Yuan Chen7Department of Food Science, National Taiwan Ocean University, Keelung 20224, TaiwanDepartment of Food Science, National Taiwan Ocean University, Keelung 20224, TaiwanInstitute of Food Safety and Risk Management, National Taiwan Ocean University, Keelung 20224, TaiwanGraduate Institute of Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, TaiwanGraduate Institute of Medical Sciences, Department of Research and Development, National Defense Medical Center, Taipei 11490, TaiwanDepartment of Nutrition &Food Science, University of Maryland, College Park, MD 20742, USADepartment of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung 811, TaiwanDepartment of Food Science, National Taiwan Ocean University, Keelung 20224, TaiwanHigh-pressure processing (HPP) is a prevailing non-thermal food preservation technology. The inactivation mechanisms of <i>Listeria monocytogenes</i> under HPP at 200 and 400 MPa for 3 min were investigated by label-free quantitative proteomic analysis and functional enrichment analysis in the Kyoto Encyclopedia of Genes and Genomes. HPP treatment at 400 MPa exhibited significant effects on proteins involved in translation, carbon, carbohydrate, lipid and energy metabolism, and peptidoglycan biosynthesis. HPP increased most ribosomal subunits and initiation factors, suggesting it might shift ribosomal biogenesis to translation initiation. However, protein synthesis was impaired by the shortage of proteins responsible for elongation, termination and recycling. HPP stimulated several ATP-dependent Clp proteases, and the global transcriptional regulator Spx, associating with activation of the stress-activated sigma factor Sigma B (σ<sup>B</sup>) and the transcriptional activator positive regulatory factor A (PrfA) regulons. The quantitative proteomics approaches provide fundamental information on <i>L. monocytogenes</i> under different HPP pressures, and provide theoretical support for HPP against Listeriosis illness and for promotion of safer ready-to-eat foods.https://www.mdpi.com/2079-7737/11/8/1152<i>Listeria monocytogenes</i>high-pressure processingquantitative proteomicstranslation initiationstress responses |
| spellingShingle | Yi-An Chen Guan-Wen Chen Hao-Hsiang Ku Tsui-Chin Huang Hsin-Yi Chang Cheng-I Wei Yung-Hsiang Tsai Tai-Yuan Chen Differential Proteomic Analysis of <em>Listeria monocytogenes</em> during High-Pressure Processing Biology <i>Listeria monocytogenes</i> high-pressure processing quantitative proteomics translation initiation stress responses |
| title | Differential Proteomic Analysis of <em>Listeria monocytogenes</em> during High-Pressure Processing |
| title_full | Differential Proteomic Analysis of <em>Listeria monocytogenes</em> during High-Pressure Processing |
| title_fullStr | Differential Proteomic Analysis of <em>Listeria monocytogenes</em> during High-Pressure Processing |
| title_full_unstemmed | Differential Proteomic Analysis of <em>Listeria monocytogenes</em> during High-Pressure Processing |
| title_short | Differential Proteomic Analysis of <em>Listeria monocytogenes</em> during High-Pressure Processing |
| title_sort | differential proteomic analysis of em listeria monocytogenes em during high pressure processing |
| topic | <i>Listeria monocytogenes</i> high-pressure processing quantitative proteomics translation initiation stress responses |
| url | https://www.mdpi.com/2079-7737/11/8/1152 |
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