Cornelian Cherry (<i>Cornus mas</i> L.) Extracts Exert Cytotoxicity in Two Selected Melanoma Cell Lines—A Factorial Analysis of Time-Dependent Alterations in Values Obtained with SRB and MTT Assays
Despite the fact that phytochemicals of <i>Cornaceae</i> species have long been discussed as possible auxiliary agents in contemporary treatment, the insights on their properties remain relatively scarce. This study focuses on <i>Cornus mas</i> L. (Cornelian cherry), the extr...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/13/4193 |
| _version_ | 1827598865070555136 |
|---|---|
| author | Łukasz Lewandowski Iwona Bednarz-Misa Alicja Z. Kucharska Agnieszka Kubiak Patrycja Kasprzyk Tomasz Sozański Dominika Przybylska Narcyz Piórecki Małgorzata Krzystek-Korpacka |
| author_facet | Łukasz Lewandowski Iwona Bednarz-Misa Alicja Z. Kucharska Agnieszka Kubiak Patrycja Kasprzyk Tomasz Sozański Dominika Przybylska Narcyz Piórecki Małgorzata Krzystek-Korpacka |
| author_sort | Łukasz Lewandowski |
| collection | DOAJ |
| description | Despite the fact that phytochemicals of <i>Cornaceae</i> species have long been discussed as possible auxiliary agents in contemporary treatment, the insights on their properties remain relatively scarce. This study focuses on <i>Cornus mas</i> L. (Cornelian cherry), the extracts of which are reported to exert a pleiotropic effect shown in both in vivo and in vitro studies. This study aimed to explore the cytotoxic effect of extracts from fruits of red (<i>Cornus mas</i> L. ‘Podolski’) and yellow (<i>Cornus mas</i> L. ‘Yantarnyi’ and ‘Flava’) Cornelian cherries on two melanoma cell lines (A375 and MeWo). The extracts were characterized in the context of the concentration of bioactive compounds of antioxidative properties. Cytotoxicity was investigated with the use of the following two assays: SRB and MTT. An additional, alternative protocol for the SRB assay was used in this study so as to account for possible bias. Cytotoxicity was assessed as a difference in the whole time series of cell viability, instead of analyzing differences in raw values (often found in the literature). Both extracts from <i>Cornus mas</i> L. induced cytotoxicity in both A375 and MeWo cell lines, although the response of these cells was different. Moreover, based on this study, there is no evidence for claiming a different magnitude of cytotoxicity between these two extracts. |
| first_indexed | 2024-03-09T04:00:56Z |
| format | Article |
| id | doaj.art-e94ad85a021a4900b4ac1168484280c5 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T04:00:56Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e94ad85a021a4900b4ac1168484280c52023-12-03T14:13:44ZengMDPI AGMolecules1420-30492022-06-012713419310.3390/molecules27134193Cornelian Cherry (<i>Cornus mas</i> L.) Extracts Exert Cytotoxicity in Two Selected Melanoma Cell Lines—A Factorial Analysis of Time-Dependent Alterations in Values Obtained with SRB and MTT AssaysŁukasz Lewandowski0Iwona Bednarz-Misa1Alicja Z. Kucharska2Agnieszka Kubiak3Patrycja Kasprzyk4Tomasz Sozański5Dominika Przybylska6Narcyz Piórecki7Małgorzata Krzystek-Korpacka8Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, PolandDepartment of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, PolandDepartment of Fruit, Vegetable and Plant Nutraceutical Technology, Wroclaw University of Environmental and Life Sciences, J. Chelmonskiego 37, 51-630 Wroclaw, PolandDepartment of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, PolandDepartment of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, PolandDepartment of Pharmacology, Wroclaw Medical University, J. Mikulicza-Radeckiego 2, 50-345 Wroclaw, PolandDepartment of Fruit, Vegetable and Plant Nutraceutical Technology, Wroclaw University of Environmental and Life Sciences, J. Chelmonskiego 37, 51-630 Wroclaw, PolandBolestraszyce Arboretum and Institute of Physiography, Bolestraszyce 130, 37-722 Wyszatyce, PolandDepartment of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, PolandDespite the fact that phytochemicals of <i>Cornaceae</i> species have long been discussed as possible auxiliary agents in contemporary treatment, the insights on their properties remain relatively scarce. This study focuses on <i>Cornus mas</i> L. (Cornelian cherry), the extracts of which are reported to exert a pleiotropic effect shown in both in vivo and in vitro studies. This study aimed to explore the cytotoxic effect of extracts from fruits of red (<i>Cornus mas</i> L. ‘Podolski’) and yellow (<i>Cornus mas</i> L. ‘Yantarnyi’ and ‘Flava’) Cornelian cherries on two melanoma cell lines (A375 and MeWo). The extracts were characterized in the context of the concentration of bioactive compounds of antioxidative properties. Cytotoxicity was investigated with the use of the following two assays: SRB and MTT. An additional, alternative protocol for the SRB assay was used in this study so as to account for possible bias. Cytotoxicity was assessed as a difference in the whole time series of cell viability, instead of analyzing differences in raw values (often found in the literature). Both extracts from <i>Cornus mas</i> L. induced cytotoxicity in both A375 and MeWo cell lines, although the response of these cells was different. Moreover, based on this study, there is no evidence for claiming a different magnitude of cytotoxicity between these two extracts.https://www.mdpi.com/1420-3049/27/13/4193A375 cell linecell culturecell viabilitycontrast analysiscornelian cherry<i>Cornus mas</i> L. |
| spellingShingle | Łukasz Lewandowski Iwona Bednarz-Misa Alicja Z. Kucharska Agnieszka Kubiak Patrycja Kasprzyk Tomasz Sozański Dominika Przybylska Narcyz Piórecki Małgorzata Krzystek-Korpacka Cornelian Cherry (<i>Cornus mas</i> L.) Extracts Exert Cytotoxicity in Two Selected Melanoma Cell Lines—A Factorial Analysis of Time-Dependent Alterations in Values Obtained with SRB and MTT Assays Molecules A375 cell line cell culture cell viability contrast analysis cornelian cherry <i>Cornus mas</i> L. |
| title | Cornelian Cherry (<i>Cornus mas</i> L.) Extracts Exert Cytotoxicity in Two Selected Melanoma Cell Lines—A Factorial Analysis of Time-Dependent Alterations in Values Obtained with SRB and MTT Assays |
| title_full | Cornelian Cherry (<i>Cornus mas</i> L.) Extracts Exert Cytotoxicity in Two Selected Melanoma Cell Lines—A Factorial Analysis of Time-Dependent Alterations in Values Obtained with SRB and MTT Assays |
| title_fullStr | Cornelian Cherry (<i>Cornus mas</i> L.) Extracts Exert Cytotoxicity in Two Selected Melanoma Cell Lines—A Factorial Analysis of Time-Dependent Alterations in Values Obtained with SRB and MTT Assays |
| title_full_unstemmed | Cornelian Cherry (<i>Cornus mas</i> L.) Extracts Exert Cytotoxicity in Two Selected Melanoma Cell Lines—A Factorial Analysis of Time-Dependent Alterations in Values Obtained with SRB and MTT Assays |
| title_short | Cornelian Cherry (<i>Cornus mas</i> L.) Extracts Exert Cytotoxicity in Two Selected Melanoma Cell Lines—A Factorial Analysis of Time-Dependent Alterations in Values Obtained with SRB and MTT Assays |
| title_sort | cornelian cherry i cornus mas i l extracts exert cytotoxicity in two selected melanoma cell lines a factorial analysis of time dependent alterations in values obtained with srb and mtt assays |
| topic | A375 cell line cell culture cell viability contrast analysis cornelian cherry <i>Cornus mas</i> L. |
| url | https://www.mdpi.com/1420-3049/27/13/4193 |
| work_keys_str_mv | AT łukaszlewandowski corneliancherryicornusmasilextractsexertcytotoxicityintwoselectedmelanomacelllinesafactorialanalysisoftimedependentalterationsinvaluesobtainedwithsrbandmttassays AT iwonabednarzmisa corneliancherryicornusmasilextractsexertcytotoxicityintwoselectedmelanomacelllinesafactorialanalysisoftimedependentalterationsinvaluesobtainedwithsrbandmttassays AT alicjazkucharska corneliancherryicornusmasilextractsexertcytotoxicityintwoselectedmelanomacelllinesafactorialanalysisoftimedependentalterationsinvaluesobtainedwithsrbandmttassays AT agnieszkakubiak corneliancherryicornusmasilextractsexertcytotoxicityintwoselectedmelanomacelllinesafactorialanalysisoftimedependentalterationsinvaluesobtainedwithsrbandmttassays AT patrycjakasprzyk corneliancherryicornusmasilextractsexertcytotoxicityintwoselectedmelanomacelllinesafactorialanalysisoftimedependentalterationsinvaluesobtainedwithsrbandmttassays AT tomaszsozanski corneliancherryicornusmasilextractsexertcytotoxicityintwoselectedmelanomacelllinesafactorialanalysisoftimedependentalterationsinvaluesobtainedwithsrbandmttassays AT dominikaprzybylska corneliancherryicornusmasilextractsexertcytotoxicityintwoselectedmelanomacelllinesafactorialanalysisoftimedependentalterationsinvaluesobtainedwithsrbandmttassays AT narcyzpiorecki corneliancherryicornusmasilextractsexertcytotoxicityintwoselectedmelanomacelllinesafactorialanalysisoftimedependentalterationsinvaluesobtainedwithsrbandmttassays AT małgorzatakrzystekkorpacka corneliancherryicornusmasilextractsexertcytotoxicityintwoselectedmelanomacelllinesafactorialanalysisoftimedependentalterationsinvaluesobtainedwithsrbandmttassays |