Valorization of Waste Glycerol to Dihydroxyacetone with Biocatalysts Obtained from <i>Gluconobacter oxydans</i>
Waste glycerol is the main by-product generated during biodiesel production, in an amount reaching up to 10% of the produced biofuel. Is there any method which allows changing this waste into industrial valuable compounds? This manuscript describes a method for valorization of crude glycerol via mic...
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MDPI AG
2018-12-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/8/12/2517 |
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| author | Lidia Stasiak-Różańska Anna Berthold-Pluta Pritam Kumar Dikshit |
| author_facet | Lidia Stasiak-Różańska Anna Berthold-Pluta Pritam Kumar Dikshit |
| author_sort | Lidia Stasiak-Różańska |
| collection | DOAJ |
| description | Waste glycerol is the main by-product generated during biodiesel production, in an amount reaching up to 10% of the produced biofuel. Is there any method which allows changing this waste into industrial valuable compounds? This manuscript describes a method for valorization of crude glycerol via microbial bioconversion. It has been shown that the use of free and immobilized biocatalysts obtained from <i>Gluconobacter oxydans</i> can enable beneficial valorization of crude glycerol to industrially valuable dihydroxyacetone. The highest concentration of this compound, reaching over 20 g·L<sup>−1</sup>, was obtained after 72 h of biotransformation with free <i>G. oxydans</i> cells, in a medium containing 30 or 50 g·L<sup>−1</sup> of waste glycerol. Using a free cell extract resulted in higher concentrations of dihydroxyacetone and a higher valorization efficiency (up to 98%) compared to the reaction with an immobilized cell extract. Increasing waste glycerol concentration to 50 g·L<sup>−1</sup> causes neither a faster nor higher increase in product yield and reaction efficiency compared to its initial concentration of 30 g·L<sup>−1</sup>. The proposed method could be an alternative for utilization of a petrochemical waste into industry applicated chemicals. |
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| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-12-13T22:30:15Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj.art-e94c757be335466b99073a82e2bf6b292022-12-21T23:29:07ZengMDPI AGApplied Sciences2076-34172018-12-01812251710.3390/app8122517app8122517Valorization of Waste Glycerol to Dihydroxyacetone with Biocatalysts Obtained from <i>Gluconobacter oxydans</i>Lidia Stasiak-Różańska0Anna Berthold-Pluta1Pritam Kumar Dikshit2Department of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences-SGGW, Nowoursynowska St. 166, 02-787 Warsaw, PolandDepartment of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences-SGGW, Nowoursynowska St. 166, 02-787 Warsaw, PolandThe Energy and Resources Institute (TERI), Darbari Seth Block, IHC Complex, Lodhi Road, New Delhi 110003, IndiaWaste glycerol is the main by-product generated during biodiesel production, in an amount reaching up to 10% of the produced biofuel. Is there any method which allows changing this waste into industrial valuable compounds? This manuscript describes a method for valorization of crude glycerol via microbial bioconversion. It has been shown that the use of free and immobilized biocatalysts obtained from <i>Gluconobacter oxydans</i> can enable beneficial valorization of crude glycerol to industrially valuable dihydroxyacetone. The highest concentration of this compound, reaching over 20 g·L<sup>−1</sup>, was obtained after 72 h of biotransformation with free <i>G. oxydans</i> cells, in a medium containing 30 or 50 g·L<sup>−1</sup> of waste glycerol. Using a free cell extract resulted in higher concentrations of dihydroxyacetone and a higher valorization efficiency (up to 98%) compared to the reaction with an immobilized cell extract. Increasing waste glycerol concentration to 50 g·L<sup>−1</sup> causes neither a faster nor higher increase in product yield and reaction efficiency compared to its initial concentration of 30 g·L<sup>−1</sup>. The proposed method could be an alternative for utilization of a petrochemical waste into industry applicated chemicals.https://www.mdpi.com/2076-3417/8/12/2517waste/crude glyceroldihydroxyacetonecell extractimmobilization<i>Gluconobacter oxydans</i>glycerol dehydrogenase |
| spellingShingle | Lidia Stasiak-Różańska Anna Berthold-Pluta Pritam Kumar Dikshit Valorization of Waste Glycerol to Dihydroxyacetone with Biocatalysts Obtained from <i>Gluconobacter oxydans</i> Applied Sciences waste/crude glycerol dihydroxyacetone cell extract immobilization <i>Gluconobacter oxydans</i> glycerol dehydrogenase |
| title | Valorization of Waste Glycerol to Dihydroxyacetone with Biocatalysts Obtained from <i>Gluconobacter oxydans</i> |
| title_full | Valorization of Waste Glycerol to Dihydroxyacetone with Biocatalysts Obtained from <i>Gluconobacter oxydans</i> |
| title_fullStr | Valorization of Waste Glycerol to Dihydroxyacetone with Biocatalysts Obtained from <i>Gluconobacter oxydans</i> |
| title_full_unstemmed | Valorization of Waste Glycerol to Dihydroxyacetone with Biocatalysts Obtained from <i>Gluconobacter oxydans</i> |
| title_short | Valorization of Waste Glycerol to Dihydroxyacetone with Biocatalysts Obtained from <i>Gluconobacter oxydans</i> |
| title_sort | valorization of waste glycerol to dihydroxyacetone with biocatalysts obtained from i gluconobacter oxydans i |
| topic | waste/crude glycerol dihydroxyacetone cell extract immobilization <i>Gluconobacter oxydans</i> glycerol dehydrogenase |
| url | https://www.mdpi.com/2076-3417/8/12/2517 |
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