Oxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell Membrane
Abstract Modulating biomolecular networks in cells with peptides and proteins has become a promising therapeutic strategy and effective biological tools. A simple and effective reagent that can bring functional proteins into cells can increase efficacy and allow more investigations. Here we show tha...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2018-02-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-018-20650-4 |
| _version_ | 1818998852637687808 |
|---|---|
| author | Kittima Amornwachirabodee Nattapol Tantimekin Porntip Pan-In Tanapat Palaga Prompong Pienpinijtham Chonlatip Pipattanaboon Thanyada Sukmanee Patcharee Ritprajak Promchat Charoenpat Pannamthip Pitaksajjakul Pongrama Ramasoota Supason Wanichwecharungruang |
| author_facet | Kittima Amornwachirabodee Nattapol Tantimekin Porntip Pan-In Tanapat Palaga Prompong Pienpinijtham Chonlatip Pipattanaboon Thanyada Sukmanee Patcharee Ritprajak Promchat Charoenpat Pannamthip Pitaksajjakul Pongrama Ramasoota Supason Wanichwecharungruang |
| author_sort | Kittima Amornwachirabodee |
| collection | DOAJ |
| description | Abstract Modulating biomolecular networks in cells with peptides and proteins has become a promising therapeutic strategy and effective biological tools. A simple and effective reagent that can bring functional proteins into cells can increase efficacy and allow more investigations. Here we show that the relatively non-toxic and non-immunogenic oxidized carbon black particles (OCBs) prepared from commercially available carbon black can deliver a 300 kDa protein directly into cells, without an involvement of a cellular endocytosis. Experiments with cell-sized liposomes indicate that OCBs directly interact with phospholipids and induce membrane leakages. Delivery of human monoclonal antibodies (HuMAbs, 150 kDa) with specific affinity towards dengue viruses (DENV) into DENV-infected Vero cells by OCBs results in HuMAbs distribution all over cells’ interior and effective viral neutralization. An ability of OCBs to deliver big functional/therapeutic proteins into cells should open doors for more protein drug investigations and new levels of antibody therapies and biological studies. |
| first_indexed | 2024-12-20T22:08:07Z |
| format | Article |
| id | doaj.art-e95249bfc79b41049602cc37c7a66f0a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T22:08:07Z |
| publishDate | 2018-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e95249bfc79b41049602cc37c7a66f0a2022-12-21T19:25:13ZengNature PortfolioScientific Reports2045-23222018-02-018111110.1038/s41598-018-20650-4Oxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell MembraneKittima Amornwachirabodee0Nattapol Tantimekin1Porntip Pan-In2Tanapat Palaga3Prompong Pienpinijtham4Chonlatip Pipattanaboon5Thanyada Sukmanee6Patcharee Ritprajak7Promchat Charoenpat8Pannamthip Pitaksajjakul9Pongrama Ramasoota10Supason Wanichwecharungruang11Department of Chemistry, Faculty of Science, Chulalongkorn UniversityDepartment of Chemistry, Faculty of Science, Chulalongkorn UniversityDepartment of Chemistry, Faculty of Science, Chulalongkorn UniversityDepartment of Microbiology, Faculty of Science, Chulalongkorn UniversityDepartment of Chemistry, Faculty of Science, Chulalongkorn UniversityCenter of Excellence for Antibody Research, Faculty of Tropical Medicine, Mahidol UniversityDepartment of Chemistry, Faculty of Science, Chulalongkorn UniversityDepartment of Microbiology, and RU in Oral Microbiology, Faculty of Dentistry, Chulalongkorn UniversityDepartment of Microbiology, and RU in Oral Microbiology, Faculty of Dentistry, Chulalongkorn UniversityCenter of Excellence for Antibody Research, and Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol UniversityCenter of Excellence for Antibody Research, and Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol UniversityDepartment of Chemistry, Faculty of Science, Chulalongkorn UniversityAbstract Modulating biomolecular networks in cells with peptides and proteins has become a promising therapeutic strategy and effective biological tools. A simple and effective reagent that can bring functional proteins into cells can increase efficacy and allow more investigations. Here we show that the relatively non-toxic and non-immunogenic oxidized carbon black particles (OCBs) prepared from commercially available carbon black can deliver a 300 kDa protein directly into cells, without an involvement of a cellular endocytosis. Experiments with cell-sized liposomes indicate that OCBs directly interact with phospholipids and induce membrane leakages. Delivery of human monoclonal antibodies (HuMAbs, 150 kDa) with specific affinity towards dengue viruses (DENV) into DENV-infected Vero cells by OCBs results in HuMAbs distribution all over cells’ interior and effective viral neutralization. An ability of OCBs to deliver big functional/therapeutic proteins into cells should open doors for more protein drug investigations and new levels of antibody therapies and biological studies.https://doi.org/10.1038/s41598-018-20650-4 |
| spellingShingle | Kittima Amornwachirabodee Nattapol Tantimekin Porntip Pan-In Tanapat Palaga Prompong Pienpinijtham Chonlatip Pipattanaboon Thanyada Sukmanee Patcharee Ritprajak Promchat Charoenpat Pannamthip Pitaksajjakul Pongrama Ramasoota Supason Wanichwecharungruang Oxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell Membrane Scientific Reports |
| title | Oxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell Membrane |
| title_full | Oxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell Membrane |
| title_fullStr | Oxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell Membrane |
| title_full_unstemmed | Oxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell Membrane |
| title_short | Oxidized Carbon Black: Preparation, Characterization and Application in Antibody Delivery across Cell Membrane |
| title_sort | oxidized carbon black preparation characterization and application in antibody delivery across cell membrane |
| url | https://doi.org/10.1038/s41598-018-20650-4 |
| work_keys_str_mv | AT kittimaamornwachirabodee oxidizedcarbonblackpreparationcharacterizationandapplicationinantibodydeliveryacrosscellmembrane AT nattapoltantimekin oxidizedcarbonblackpreparationcharacterizationandapplicationinantibodydeliveryacrosscellmembrane AT porntippanin oxidizedcarbonblackpreparationcharacterizationandapplicationinantibodydeliveryacrosscellmembrane AT tanapatpalaga oxidizedcarbonblackpreparationcharacterizationandapplicationinantibodydeliveryacrosscellmembrane AT prompongpienpinijtham oxidizedcarbonblackpreparationcharacterizationandapplicationinantibodydeliveryacrosscellmembrane AT chonlatippipattanaboon oxidizedcarbonblackpreparationcharacterizationandapplicationinantibodydeliveryacrosscellmembrane AT thanyadasukmanee oxidizedcarbonblackpreparationcharacterizationandapplicationinantibodydeliveryacrosscellmembrane AT patchareeritprajak oxidizedcarbonblackpreparationcharacterizationandapplicationinantibodydeliveryacrosscellmembrane AT promchatcharoenpat oxidizedcarbonblackpreparationcharacterizationandapplicationinantibodydeliveryacrosscellmembrane AT pannamthippitaksajjakul oxidizedcarbonblackpreparationcharacterizationandapplicationinantibodydeliveryacrosscellmembrane AT pongramaramasoota oxidizedcarbonblackpreparationcharacterizationandapplicationinantibodydeliveryacrosscellmembrane AT supasonwanichwecharungruang oxidizedcarbonblackpreparationcharacterizationandapplicationinantibodydeliveryacrosscellmembrane |