Identification of extremely GC-rich micro RNAs for RT-qPCR data normalization in human plasma

Identification of extremely GC-rich micro RNAs for RT-qPCR data normalization in human plasma

We aimed at extending the repertoire of high-quality miRNA normalizers for reverse transcription-quantitative PCR (RT-qPCR) of human plasma with special emphasis on the extremely guanine-cytosine-rich portion of the miRNome. For high-throughput selection of stable candidates, microarray technology w...

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Bibliographic Details
Main Authors: Volker Baumann, Angelos-Theodoros Athanasiou, Omid R. Faridani, Andreas R. Schwerdtfeger, Bernard Wallner, Ralf Steinborn
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-01-01
Series:Frontiers in Genetics
Subjects:
miRNA expression microarray
small-RNA sequencing
stem-loop reverse-transcription quantitative PCR
human plasma miRNAs
miRNA reference genes
cognitive stress-coping
Online Access:https://www.frontiersin.org/articles/10.3389/fgene.2022.1058668/full

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https://www.frontiersin.org/articles/10.3389/fgene.2022.1058668/full

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