Determination of HLA-A, -B and -DRB1 polymorphism in brain dead organ donors representative of the Colombian general population, 2007-2014
Introduction: Genes encoding for human leukocyte antigens (HLA) are highly polymorphic and of great importance in organ transplantation procedures, as determining allelic frequencies in defined populations is taken into account among the scientific criteria for organ allocation. Objective: The objec...
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| Format: | Article |
| Language: | English |
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Instituto Nacional de Salud
2017-06-01
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| Series: | Biomédica: revista del Instituto Nacional de Salud |
| Subjects: | |
| Online Access: | https://www.revistabiomedica.org/index.php/biomedica/article/view/3263 |
| _version_ | 1818012500900184064 |
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| author | Yazmin Rocío Arias Karime Osorio-Arango Brayan Bayona Guadalupe Ercilla Mauricio Beltrán-Durán |
| author_facet | Yazmin Rocío Arias Karime Osorio-Arango Brayan Bayona Guadalupe Ercilla Mauricio Beltrán-Durán |
| author_sort | Yazmin Rocío Arias |
| collection | DOAJ |
| description | Introduction: Genes encoding for human leukocyte antigens (HLA) are highly polymorphic and of great importance in organ transplantation procedures, as determining allelic frequencies in defined populations is taken into account among the scientific criteria for organ allocation.
Objective: The objective of this study was to establish the antigen HLA-A, -B, and -DRB1 haplotype frequencies in organ donors representative of the Colombian population after brain death.
Materials and methods: We conducted a descriptive retrospective study involving 2,506 cadaveric organ donors including an allelic and haplotype analysis of HLA-A, -B and -DRB1; we also determined the Hardy-Weinberg equilibrium.
Results: We identified 21, 43 and 15 allelic loci for groups A*, B* and DRB1*, respectively. We detected 1,268 HLA-A, -B and -DR, 409 HLA-A-B, 383 HLA-DR-B, and 218 HLA-A-DR haplotypes. The three loci were found to be in Hardy-Weinberg equilibrium between the number of heterozygotes observed and the expected number, with p values of <0.05.
Conclusions: This study provides information on the allelic distribution of HLA class I and II in organ donors from the six regions in which Colombia is structurally divided to provide transplant services. |
| first_indexed | 2024-04-14T06:20:59Z |
| format | Article |
| id | doaj.art-e95a10e9de904d10a5c0aed033321c46 |
| institution | Directory Open Access Journal |
| issn | 0120-4157 0120-4157 |
| language | English |
| last_indexed | 2024-04-14T06:20:59Z |
| publishDate | 2017-06-01 |
| publisher | Instituto Nacional de Salud |
| record_format | Article |
| series | Biomédica: revista del Instituto Nacional de Salud |
| spelling | doaj.art-e95a10e9de904d10a5c0aed033321c462022-12-22T02:08:01ZengInstituto Nacional de SaludBiomédica: revista del Instituto Nacional de Salud0120-41570120-41572017-06-0137218419010.7705/biomedica.v37i2.32631959Determination of HLA-A, -B and -DRB1 polymorphism in brain dead organ donors representative of the Colombian general population, 2007-2014Yazmin Rocío Arias0Karime Osorio-Arango1Brayan Bayona2Guadalupe Ercilla3Mauricio Beltrán-Durán4Coordinación Red Nacional de Donación y Trasplantes, Dirección Redes en Salud Pública, Instituto Nacional de Salud, Bogotá, D.C., ColombiaSubdirección Red Nacional de Trasplantes y Bancos de Sangre, Dirección Redes en Salud Pública, Instituto Nacional de Salud, Bogotá, D.C., ColombiaCentro de Diagnóstico Especializado, Universidad del Norte, Barranquilla, ColombiaServicio de Inmunología, Hospital Clinic de Barcelona, Barcelona, EspañaDirección Redes en Salud Pública, Instituto Nacional de Salud, Bogotá, D.C., ColombiaIntroduction: Genes encoding for human leukocyte antigens (HLA) are highly polymorphic and of great importance in organ transplantation procedures, as determining allelic frequencies in defined populations is taken into account among the scientific criteria for organ allocation. Objective: The objective of this study was to establish the antigen HLA-A, -B, and -DRB1 haplotype frequencies in organ donors representative of the Colombian population after brain death. Materials and methods: We conducted a descriptive retrospective study involving 2,506 cadaveric organ donors including an allelic and haplotype analysis of HLA-A, -B and -DRB1; we also determined the Hardy-Weinberg equilibrium. Results: We identified 21, 43 and 15 allelic loci for groups A*, B* and DRB1*, respectively. We detected 1,268 HLA-A, -B and -DR, 409 HLA-A-B, 383 HLA-DR-B, and 218 HLA-A-DR haplotypes. The three loci were found to be in Hardy-Weinberg equilibrium between the number of heterozygotes observed and the expected number, with p values of <0.05. Conclusions: This study provides information on the allelic distribution of HLA class I and II in organ donors from the six regions in which Colombia is structurally divided to provide transplant services.https://www.revistabiomedica.org/index.php/biomedica/article/view/3263HLA antigensalleleshaplotypestransplantationtissue donor, Colombia |
| spellingShingle | Yazmin Rocío Arias Karime Osorio-Arango Brayan Bayona Guadalupe Ercilla Mauricio Beltrán-Durán Determination of HLA-A, -B and -DRB1 polymorphism in brain dead organ donors representative of the Colombian general population, 2007-2014 Biomédica: revista del Instituto Nacional de Salud HLA antigens alleles haplotypes transplantation tissue donor, Colombia |
| title | Determination of HLA-A, -B and -DRB1 polymorphism in brain dead organ donors representative of the Colombian general population, 2007-2014 |
| title_full | Determination of HLA-A, -B and -DRB1 polymorphism in brain dead organ donors representative of the Colombian general population, 2007-2014 |
| title_fullStr | Determination of HLA-A, -B and -DRB1 polymorphism in brain dead organ donors representative of the Colombian general population, 2007-2014 |
| title_full_unstemmed | Determination of HLA-A, -B and -DRB1 polymorphism in brain dead organ donors representative of the Colombian general population, 2007-2014 |
| title_short | Determination of HLA-A, -B and -DRB1 polymorphism in brain dead organ donors representative of the Colombian general population, 2007-2014 |
| title_sort | determination of hla a b and drb1 polymorphism in brain dead organ donors representative of the colombian general population 2007 2014 |
| topic | HLA antigens alleles haplotypes transplantation tissue donor, Colombia |
| url | https://www.revistabiomedica.org/index.php/biomedica/article/view/3263 |
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