AQP5 regulates the proliferation and differentiation of epidermal stem cells in skin aging

The epidermis, the outermost layer of the skin, is the first barrier that comes into contact with the external environment. It plays an important role in resisting the invasion of harmful substances and microbial infections. The skin changes with age and external environmental factors. This study ai...

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Main Authors: Jing Zhou, Yabing Dong, Jianlan Liu, Jie Ren, Jinyan Wu, Ningwen Zhu
Format: Article
Language:English
Published: Associação Brasileira de Divulgação Científica 2020-09-01
Series:Brazilian Journal of Medical and Biological Research
Subjects:
Online Access:http://www.scielo.br/pdf/bjmbr/v53n11/1414-431X-bjmbr-53-11-e10009.pdf
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author Jing Zhou
Yabing Dong
Jianlan Liu
Jie Ren
Jinyan Wu
Ningwen Zhu
author_facet Jing Zhou
Yabing Dong
Jianlan Liu
Jie Ren
Jinyan Wu
Ningwen Zhu
author_sort Jing Zhou
collection DOAJ
description The epidermis, the outermost layer of the skin, is the first barrier that comes into contact with the external environment. It plays an important role in resisting the invasion of harmful substances and microbial infections. The skin changes with age and external environmental factors. This study aimed to investigate epidermal stem cells during the process of aging. This study enrolled 9 volunteers with benign pigmented nevus for clinical dermatologic surgery. The phenotypes associated with skin aging changes such as skin wrinkles and elasticity of the unexposed/healthy parts near benign pigmented skin were measured, and epidermal stem cells from this region were isolated for transcriptome sequencing. The results showed that epidermal stem cells could be obtained by magnetic activated cell sorting (MACS) with high purity. Results of the transcriptome sequencing revealed that aquaporin (AQP)5 significantly decreased in the epidermal stem cells with age, and further functional experiments revealed that AQP5 could promote the proliferation and dedifferentiation of HaCaT, but did not influence cell apoptosis. In summary, AQP5 regulated the proliferation and differentiation of epidermal stem cells in skin aging, and it may play an important role in the balance of proliferation and differentiation. However, further studies are needed to determine the mechanism by which AQP5 regulates the proliferation and differentiation of epidermal skin cells in aging.
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spelling doaj.art-e95b76a4a10e47ccb35bd0b8c5747c2b2022-12-21T17:49:15ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2020-09-01531110.1590/1414-431x202010009AQP5 regulates the proliferation and differentiation of epidermal stem cells in skin agingJing Zhouhttps://orcid.org/0000-0002-0214-0870Yabing Donghttps://orcid.org/0000-0003-1008-4853Jianlan Liuhttps://orcid.org/0000-0003-0994-6401Jie Renhttps://orcid.org/0000-0001-8831-5899Jinyan Wuhttps://orcid.org/0000-0002-9133-2304Ningwen Zhuhttps://orcid.org/0000-0003-4224-4188The epidermis, the outermost layer of the skin, is the first barrier that comes into contact with the external environment. It plays an important role in resisting the invasion of harmful substances and microbial infections. The skin changes with age and external environmental factors. This study aimed to investigate epidermal stem cells during the process of aging. This study enrolled 9 volunteers with benign pigmented nevus for clinical dermatologic surgery. The phenotypes associated with skin aging changes such as skin wrinkles and elasticity of the unexposed/healthy parts near benign pigmented skin were measured, and epidermal stem cells from this region were isolated for transcriptome sequencing. The results showed that epidermal stem cells could be obtained by magnetic activated cell sorting (MACS) with high purity. Results of the transcriptome sequencing revealed that aquaporin (AQP)5 significantly decreased in the epidermal stem cells with age, and further functional experiments revealed that AQP5 could promote the proliferation and dedifferentiation of HaCaT, but did not influence cell apoptosis. In summary, AQP5 regulated the proliferation and differentiation of epidermal stem cells in skin aging, and it may play an important role in the balance of proliferation and differentiation. However, further studies are needed to determine the mechanism by which AQP5 regulates the proliferation and differentiation of epidermal skin cells in aging.http://www.scielo.br/pdf/bjmbr/v53n11/1414-431X-bjmbr-53-11-e10009.pdfSkin agingEpidermal stem cellAQP5ProliferationDifferentiation
spellingShingle Jing Zhou
Yabing Dong
Jianlan Liu
Jie Ren
Jinyan Wu
Ningwen Zhu
AQP5 regulates the proliferation and differentiation of epidermal stem cells in skin aging
Brazilian Journal of Medical and Biological Research
Skin aging
Epidermal stem cell
AQP5
Proliferation
Differentiation
title AQP5 regulates the proliferation and differentiation of epidermal stem cells in skin aging
title_full AQP5 regulates the proliferation and differentiation of epidermal stem cells in skin aging
title_fullStr AQP5 regulates the proliferation and differentiation of epidermal stem cells in skin aging
title_full_unstemmed AQP5 regulates the proliferation and differentiation of epidermal stem cells in skin aging
title_short AQP5 regulates the proliferation and differentiation of epidermal stem cells in skin aging
title_sort aqp5 regulates the proliferation and differentiation of epidermal stem cells in skin aging
topic Skin aging
Epidermal stem cell
AQP5
Proliferation
Differentiation
url http://www.scielo.br/pdf/bjmbr/v53n11/1414-431X-bjmbr-53-11-e10009.pdf
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