AQP5 regulates the proliferation and differentiation of epidermal stem cells in skin aging
The epidermis, the outermost layer of the skin, is the first barrier that comes into contact with the external environment. It plays an important role in resisting the invasion of harmful substances and microbial infections. The skin changes with age and external environmental factors. This study ai...
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Format: | Article |
Language: | English |
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Associação Brasileira de Divulgação Científica
2020-09-01
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Series: | Brazilian Journal of Medical and Biological Research |
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Online Access: | http://www.scielo.br/pdf/bjmbr/v53n11/1414-431X-bjmbr-53-11-e10009.pdf |
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author | Jing Zhou Yabing Dong Jianlan Liu Jie Ren Jinyan Wu Ningwen Zhu |
author_facet | Jing Zhou Yabing Dong Jianlan Liu Jie Ren Jinyan Wu Ningwen Zhu |
author_sort | Jing Zhou |
collection | DOAJ |
description | The epidermis, the outermost layer of the skin, is the first barrier that comes into contact with the external environment. It plays an important role in resisting the invasion of harmful substances and microbial infections. The skin changes with age and external environmental factors. This study aimed to investigate epidermal stem cells during the process of aging. This study enrolled 9 volunteers with benign pigmented nevus for clinical dermatologic surgery. The phenotypes associated with skin aging changes such as skin wrinkles and elasticity of the unexposed/healthy parts near benign pigmented skin were measured, and epidermal stem cells from this region were isolated for transcriptome sequencing. The results showed that epidermal stem cells could be obtained by magnetic activated cell sorting (MACS) with high purity. Results of the transcriptome sequencing revealed that aquaporin (AQP)5 significantly decreased in the epidermal stem cells with age, and further functional experiments revealed that AQP5 could promote the proliferation and dedifferentiation of HaCaT, but did not influence cell apoptosis. In summary, AQP5 regulated the proliferation and differentiation of epidermal stem cells in skin aging, and it may play an important role in the balance of proliferation and differentiation. However, further studies are needed to determine the mechanism by which AQP5 regulates the proliferation and differentiation of epidermal skin cells in aging. |
first_indexed | 2024-12-23T11:14:45Z |
format | Article |
id | doaj.art-e95b76a4a10e47ccb35bd0b8c5747c2b |
institution | Directory Open Access Journal |
issn | 1414-431X |
language | English |
last_indexed | 2024-12-23T11:14:45Z |
publishDate | 2020-09-01 |
publisher | Associação Brasileira de Divulgação Científica |
record_format | Article |
series | Brazilian Journal of Medical and Biological Research |
spelling | doaj.art-e95b76a4a10e47ccb35bd0b8c5747c2b2022-12-21T17:49:15ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2020-09-01531110.1590/1414-431x202010009AQP5 regulates the proliferation and differentiation of epidermal stem cells in skin agingJing Zhouhttps://orcid.org/0000-0002-0214-0870Yabing Donghttps://orcid.org/0000-0003-1008-4853Jianlan Liuhttps://orcid.org/0000-0003-0994-6401Jie Renhttps://orcid.org/0000-0001-8831-5899Jinyan Wuhttps://orcid.org/0000-0002-9133-2304Ningwen Zhuhttps://orcid.org/0000-0003-4224-4188The epidermis, the outermost layer of the skin, is the first barrier that comes into contact with the external environment. It plays an important role in resisting the invasion of harmful substances and microbial infections. The skin changes with age and external environmental factors. This study aimed to investigate epidermal stem cells during the process of aging. This study enrolled 9 volunteers with benign pigmented nevus for clinical dermatologic surgery. The phenotypes associated with skin aging changes such as skin wrinkles and elasticity of the unexposed/healthy parts near benign pigmented skin were measured, and epidermal stem cells from this region were isolated for transcriptome sequencing. The results showed that epidermal stem cells could be obtained by magnetic activated cell sorting (MACS) with high purity. Results of the transcriptome sequencing revealed that aquaporin (AQP)5 significantly decreased in the epidermal stem cells with age, and further functional experiments revealed that AQP5 could promote the proliferation and dedifferentiation of HaCaT, but did not influence cell apoptosis. In summary, AQP5 regulated the proliferation and differentiation of epidermal stem cells in skin aging, and it may play an important role in the balance of proliferation and differentiation. However, further studies are needed to determine the mechanism by which AQP5 regulates the proliferation and differentiation of epidermal skin cells in aging.http://www.scielo.br/pdf/bjmbr/v53n11/1414-431X-bjmbr-53-11-e10009.pdfSkin agingEpidermal stem cellAQP5ProliferationDifferentiation |
spellingShingle | Jing Zhou Yabing Dong Jianlan Liu Jie Ren Jinyan Wu Ningwen Zhu AQP5 regulates the proliferation and differentiation of epidermal stem cells in skin aging Brazilian Journal of Medical and Biological Research Skin aging Epidermal stem cell AQP5 Proliferation Differentiation |
title | AQP5 regulates the proliferation and differentiation of epidermal stem cells in skin aging |
title_full | AQP5 regulates the proliferation and differentiation of epidermal stem cells in skin aging |
title_fullStr | AQP5 regulates the proliferation and differentiation of epidermal stem cells in skin aging |
title_full_unstemmed | AQP5 regulates the proliferation and differentiation of epidermal stem cells in skin aging |
title_short | AQP5 regulates the proliferation and differentiation of epidermal stem cells in skin aging |
title_sort | aqp5 regulates the proliferation and differentiation of epidermal stem cells in skin aging |
topic | Skin aging Epidermal stem cell AQP5 Proliferation Differentiation |
url | http://www.scielo.br/pdf/bjmbr/v53n11/1414-431X-bjmbr-53-11-e10009.pdf |
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