<i>Plectranthus zeylanicus</i>: A Rich Source of Secondary Metabolites with Antimicrobial, Disinfectant and Anti-Inflammatory Activities
<i>Plectranthus zeylanicus</i> Benth is used in Sri Lankan folk medicine as a remedy for inflammatory conditions and microbial infections. Our previous investigations revealed potent 5-lipoxygenase (5-LO) inhibitory activity in lipophilic extracts of this plant, supporting its anti-infla...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/15/4/436 |
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| author | Mayuri Napagoda Jana Gerstmeier Hannah Butschek Sybille Lorenz Sudhara De Soyza Mallique Qader Ajith Nagahawatte Gaya Bandara Wijayaratne Bernd Schneider Aleš Svatoš Lalith Jayasinghe Andreas Koeberle Oliver Werz |
| author_facet | Mayuri Napagoda Jana Gerstmeier Hannah Butschek Sybille Lorenz Sudhara De Soyza Mallique Qader Ajith Nagahawatte Gaya Bandara Wijayaratne Bernd Schneider Aleš Svatoš Lalith Jayasinghe Andreas Koeberle Oliver Werz |
| author_sort | Mayuri Napagoda |
| collection | DOAJ |
| description | <i>Plectranthus zeylanicus</i> Benth is used in Sri Lankan folk medicine as a remedy for inflammatory conditions and microbial infections. Our previous investigations revealed potent 5-lipoxygenase (5-LO) inhibitory activity in lipophilic extracts of this plant, supporting its anti-inflammatory potential. In-depth studies on the antimicrobial activity have not been conducted and the bioactive ingredients remained elusive. As a continuation of our previous work, the present investigation was undertaken to evaluate the antimicrobial activity of different extracts of <i>P. zeylanicus</i> and to isolate and characterize bioactive secondary metabolites. Different organic extracts of this plant were analyzed for their antibacterial activity, and the most active extract, i.e., dichloromethane extract, was subjected to bioactivity-guided fractionation, which led to the isolation of 7α-acetoxy-6β-hydroxyroyleanone. This compound displayed strong antibacterial activity against methicillin-resistant <i>Staphylococcus aureus</i> with a minimum inhibitory concentration of 62.5 µg/mL, and its disinfectant capacity was comparable to the potency of a commercial disinfectant. Moreover, 7α-acetoxy-6β-hydroxyroyleanone inhibits 5-LO with IC<sub>50</sub> values of 1.3 and 5.1 µg/mL in cell-free and cell-based assays, respectively. These findings rationalize the ethnopharmacological use of <i>P. zeylanicus</i> as antimicrobial and anti-inflammatory remedy. |
| first_indexed | 2024-03-09T13:11:06Z |
| format | Article |
| id | doaj.art-e95b9822115e45f5a4820ca3d3f99893 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-09T13:11:06Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-e95b9822115e45f5a4820ca3d3f998932023-11-30T21:43:01ZengMDPI AGPharmaceuticals1424-82472022-03-0115443610.3390/ph15040436<i>Plectranthus zeylanicus</i>: A Rich Source of Secondary Metabolites with Antimicrobial, Disinfectant and Anti-Inflammatory ActivitiesMayuri Napagoda0Jana Gerstmeier1Hannah Butschek2Sybille Lorenz3Sudhara De Soyza4Mallique Qader5Ajith Nagahawatte6Gaya Bandara Wijayaratne7Bernd Schneider8Aleš Svatoš9Lalith Jayasinghe10Andreas Koeberle11Oliver Werz12Department of Biochemistry, Faculty of Medicine, University of Ruhuna, Galle 80000, Sri LankaDepartment of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich Schiller University Jena, Philosophenweg 14, D-07743 Jena, GermanyDepartment of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich Schiller University Jena, Philosophenweg 14, D-07743 Jena, GermanyResearch Group Mass Spectrometry and Proteomics, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, D-07745 Jena, GermanyDepartment of Biochemistry, Faculty of Medicine, University of Ruhuna, Galle 80000, Sri LankaNatural Products Research Division, National Institute of Fundamental Studies, Hantana Road, Kandy 20000, Sri LankaDepartment of Microbiology, Faculty of Medicine, University of Ruhuna, Galle 80000, Sri LankaDepartment of Microbiology, Faculty of Medicine, University of Ruhuna, Galle 80000, Sri LankaResearch Group Biosynthesis/NMR, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, D-07745 Jena, GermanyResearch Group Mass Spectrometry and Proteomics, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, D-07745 Jena, GermanyNatural Products Research Division, National Institute of Fundamental Studies, Hantana Road, Kandy 20000, Sri LankaDepartment of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich Schiller University Jena, Philosophenweg 14, D-07743 Jena, GermanyDepartment of Pharmaceutical/Medicinal Chemistry, Institute of Pharmacy, Friedrich Schiller University Jena, Philosophenweg 14, D-07743 Jena, Germany<i>Plectranthus zeylanicus</i> Benth is used in Sri Lankan folk medicine as a remedy for inflammatory conditions and microbial infections. Our previous investigations revealed potent 5-lipoxygenase (5-LO) inhibitory activity in lipophilic extracts of this plant, supporting its anti-inflammatory potential. In-depth studies on the antimicrobial activity have not been conducted and the bioactive ingredients remained elusive. As a continuation of our previous work, the present investigation was undertaken to evaluate the antimicrobial activity of different extracts of <i>P. zeylanicus</i> and to isolate and characterize bioactive secondary metabolites. Different organic extracts of this plant were analyzed for their antibacterial activity, and the most active extract, i.e., dichloromethane extract, was subjected to bioactivity-guided fractionation, which led to the isolation of 7α-acetoxy-6β-hydroxyroyleanone. This compound displayed strong antibacterial activity against methicillin-resistant <i>Staphylococcus aureus</i> with a minimum inhibitory concentration of 62.5 µg/mL, and its disinfectant capacity was comparable to the potency of a commercial disinfectant. Moreover, 7α-acetoxy-6β-hydroxyroyleanone inhibits 5-LO with IC<sub>50</sub> values of 1.3 and 5.1 µg/mL in cell-free and cell-based assays, respectively. These findings rationalize the ethnopharmacological use of <i>P. zeylanicus</i> as antimicrobial and anti-inflammatory remedy.https://www.mdpi.com/1424-8247/15/4/436anti-inflammatoryantimicrobialdisinfectant<i>Plectranthus zeylanicus</i> |
| spellingShingle | Mayuri Napagoda Jana Gerstmeier Hannah Butschek Sybille Lorenz Sudhara De Soyza Mallique Qader Ajith Nagahawatte Gaya Bandara Wijayaratne Bernd Schneider Aleš Svatoš Lalith Jayasinghe Andreas Koeberle Oliver Werz <i>Plectranthus zeylanicus</i>: A Rich Source of Secondary Metabolites with Antimicrobial, Disinfectant and Anti-Inflammatory Activities Pharmaceuticals anti-inflammatory antimicrobial disinfectant <i>Plectranthus zeylanicus</i> |
| title | <i>Plectranthus zeylanicus</i>: A Rich Source of Secondary Metabolites with Antimicrobial, Disinfectant and Anti-Inflammatory Activities |
| title_full | <i>Plectranthus zeylanicus</i>: A Rich Source of Secondary Metabolites with Antimicrobial, Disinfectant and Anti-Inflammatory Activities |
| title_fullStr | <i>Plectranthus zeylanicus</i>: A Rich Source of Secondary Metabolites with Antimicrobial, Disinfectant and Anti-Inflammatory Activities |
| title_full_unstemmed | <i>Plectranthus zeylanicus</i>: A Rich Source of Secondary Metabolites with Antimicrobial, Disinfectant and Anti-Inflammatory Activities |
| title_short | <i>Plectranthus zeylanicus</i>: A Rich Source of Secondary Metabolites with Antimicrobial, Disinfectant and Anti-Inflammatory Activities |
| title_sort | i plectranthus zeylanicus i a rich source of secondary metabolites with antimicrobial disinfectant and anti inflammatory activities |
| topic | anti-inflammatory antimicrobial disinfectant <i>Plectranthus zeylanicus</i> |
| url | https://www.mdpi.com/1424-8247/15/4/436 |
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