The Effect of Gestational Exposure of Sodium Cromoglycate on Epileptiform Activities in the Rat Offspring
Background: Epilepsy is one of the most common neurological diseases globally. Mast cells are known to be the main source of brain histamine, which is identified as being involved in seizure modulation. Sodium cromoglycate is a popular mast cell stabilizer and is proposed as the first line therapy...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Tehran
2018-08-01
|
| Series: | Iranian Journal of Veterinary Medicine |
| Subjects: | |
| Online Access: | https://ijvm.ut.ac.ir/article_67115_5a53ecea7f36615ad8b558a6099173de.pdf |
| _version_ | 1818979972402905088 |
|---|---|
| author | Midia Kamali Morteza Zendedel Vahab Babapour Behnam Heshmatian |
| author_facet | Midia Kamali Morteza Zendedel Vahab Babapour Behnam Heshmatian |
| author_sort | Midia Kamali |
| collection | DOAJ |
| description | Background: Epilepsy is one of the most common neurological diseases globally. Mast cells are known to be the main source of brain histamine, which is identified as being involved in seizure modulation. Sodium cromoglycate is a popular mast cell stabilizer and is proposed as the first line therapy in chronic asthma and systemic mastocytosis. Considering the importance of gestational period in brain formation and given the apparent role of the histaminergic system in developmental stages, the question arises as to whether gestational exposure to sodium cromoglycate can impact epileptic susceptibility in the offspring. OBJECTIVES: The aim of this study was to evaluate the effects of gestational exposure of sodium cromoglycate on pentylenetetrazole-induced epileptiform activities in the rat offspring. METHODS: Twelve pregnant Wistar rats were divided into 3 groups (n=4) including: two treatment groups which received intraperitoneal Sodium cromoglycate in doses of 25 and 50 mg/kg once daily at the last week of pregnancy, and the control group which only received Phosphate-buffered saline at the same amount (0.5 ml) and the same order. At postnatal day 12, eight male pups were selected from each group’s offspring. Anesthetized pups were transferred to stereotaxic frame and silver electrodes were implanted surgically over the brain cortex. After recovery, pups were placed into the recording chamber and pentylenetetrazole-induced epileptiform activities were measured. RESULTS: Electrographic data showed a decrement in seizure latency and increment in frequency/amplitude of the spikes in sodium cromoglycate groups in comparison to the control group (p<0.05). Also, Parallel behavioral observations were consistent with the electrographic data. CONCLUSIONS: The obtained results reveal enhanced epileptiform activity in developing rats due to prenatal exposure to sodium cromoglycate |
| first_indexed | 2024-12-20T17:08:01Z |
| format | Article |
| id | doaj.art-e95d485e453b40558f070e24355041cd |
| institution | Directory Open Access Journal |
| issn | 2251-8894 2252-0554 |
| language | English |
| last_indexed | 2024-12-20T17:08:01Z |
| publishDate | 2018-08-01 |
| publisher | University of Tehran |
| record_format | Article |
| series | Iranian Journal of Veterinary Medicine |
| spelling | doaj.art-e95d485e453b40558f070e24355041cd2022-12-21T19:32:13ZengUniversity of TehranIranian Journal of Veterinary Medicine2251-88942252-05542018-08-0112322723510.22059/ijvm.2018.252228.100487767115The Effect of Gestational Exposure of Sodium Cromoglycate on Epileptiform Activities in the Rat OffspringMidia Kamali0Morteza Zendedel1Vahab Babapour2Behnam Heshmatian3Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranDepartment of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranDepartment of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranNeurophysiology Research Center, Urmia University of Medical Sciences, Urmia, IranBackground: Epilepsy is one of the most common neurological diseases globally. Mast cells are known to be the main source of brain histamine, which is identified as being involved in seizure modulation. Sodium cromoglycate is a popular mast cell stabilizer and is proposed as the first line therapy in chronic asthma and systemic mastocytosis. Considering the importance of gestational period in brain formation and given the apparent role of the histaminergic system in developmental stages, the question arises as to whether gestational exposure to sodium cromoglycate can impact epileptic susceptibility in the offspring. OBJECTIVES: The aim of this study was to evaluate the effects of gestational exposure of sodium cromoglycate on pentylenetetrazole-induced epileptiform activities in the rat offspring. METHODS: Twelve pregnant Wistar rats were divided into 3 groups (n=4) including: two treatment groups which received intraperitoneal Sodium cromoglycate in doses of 25 and 50 mg/kg once daily at the last week of pregnancy, and the control group which only received Phosphate-buffered saline at the same amount (0.5 ml) and the same order. At postnatal day 12, eight male pups were selected from each group’s offspring. Anesthetized pups were transferred to stereotaxic frame and silver electrodes were implanted surgically over the brain cortex. After recovery, pups were placed into the recording chamber and pentylenetetrazole-induced epileptiform activities were measured. RESULTS: Electrographic data showed a decrement in seizure latency and increment in frequency/amplitude of the spikes in sodium cromoglycate groups in comparison to the control group (p<0.05). Also, Parallel behavioral observations were consistent with the electrographic data. CONCLUSIONS: The obtained results reveal enhanced epileptiform activity in developing rats due to prenatal exposure to sodium cromoglycatehttps://ijvm.ut.ac.ir/article_67115_5a53ecea7f36615ad8b558a6099173de.pdfepileptiform activitygestational exposureoffspringratsodium cromoglycate |
| spellingShingle | Midia Kamali Morteza Zendedel Vahab Babapour Behnam Heshmatian The Effect of Gestational Exposure of Sodium Cromoglycate on Epileptiform Activities in the Rat Offspring Iranian Journal of Veterinary Medicine epileptiform activity gestational exposure offspring rat sodium cromoglycate |
| title | The Effect of Gestational Exposure of Sodium Cromoglycate on Epileptiform Activities in the Rat Offspring |
| title_full | The Effect of Gestational Exposure of Sodium Cromoglycate on Epileptiform Activities in the Rat Offspring |
| title_fullStr | The Effect of Gestational Exposure of Sodium Cromoglycate on Epileptiform Activities in the Rat Offspring |
| title_full_unstemmed | The Effect of Gestational Exposure of Sodium Cromoglycate on Epileptiform Activities in the Rat Offspring |
| title_short | The Effect of Gestational Exposure of Sodium Cromoglycate on Epileptiform Activities in the Rat Offspring |
| title_sort | effect of gestational exposure of sodium cromoglycate on epileptiform activities in the rat offspring |
| topic | epileptiform activity gestational exposure offspring rat sodium cromoglycate |
| url | https://ijvm.ut.ac.ir/article_67115_5a53ecea7f36615ad8b558a6099173de.pdf |
| work_keys_str_mv | AT midiakamali theeffectofgestationalexposureofsodiumcromoglycateonepileptiformactivitiesintheratoffspring AT mortezazendedel theeffectofgestationalexposureofsodiumcromoglycateonepileptiformactivitiesintheratoffspring AT vahabbabapour theeffectofgestationalexposureofsodiumcromoglycateonepileptiformactivitiesintheratoffspring AT behnamheshmatian theeffectofgestationalexposureofsodiumcromoglycateonepileptiformactivitiesintheratoffspring AT midiakamali effectofgestationalexposureofsodiumcromoglycateonepileptiformactivitiesintheratoffspring AT mortezazendedel effectofgestationalexposureofsodiumcromoglycateonepileptiformactivitiesintheratoffspring AT vahabbabapour effectofgestationalexposureofsodiumcromoglycateonepileptiformactivitiesintheratoffspring AT behnamheshmatian effectofgestationalexposureofsodiumcromoglycateonepileptiformactivitiesintheratoffspring |