Humulene Inhibits Acute Gastric Mucosal Injury by Enhancing Mucosal Integrity
This study was designed to determine whether α-humulene, a major constituent in many plants used in fragrances, has a protective role against gastric injury in vivo and in vitro. A rat model of hydrochloric acid (HCl)/ethanol-induced gastritis and human mast cells (HMC-1) were used to investigate th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/5/761 |
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| author | Dahee Yeo Su-Jung Hwang Ye-Seul Song Hyo-Jong Lee |
| author_facet | Dahee Yeo Su-Jung Hwang Ye-Seul Song Hyo-Jong Lee |
| author_sort | Dahee Yeo |
| collection | DOAJ |
| description | This study was designed to determine whether α-humulene, a major constituent in many plants used in fragrances, has a protective role against gastric injury in vivo and in vitro. A rat model of hydrochloric acid (HCl)/ethanol-induced gastritis and human mast cells (HMC-1) were used to investigate the mucosal protective effect of α-humulene. α-Humulene significantly inhibited gastric lesions in HCl/ethanol-induced acute gastritis and decreased gastric acid secretion pyloric ligation-induced gastric ulcers in vivo. In addition, α-humulene reduced the amount of reactive oxygen species and malondialdehyde through upregulation of prostaglandin E2 (PGE2) and superoxide dismutase (SOD). In HMC-1 cells, α-humulene decreased intracellular calcium and increased intracellular cyclic adenosine monophosphate (cAMP) levels, resulting in low histamine levels. α-Humulene also reduced the expression levels of cytokine genes such as interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF) by downregulating nuclear factor-κB (NF-κB) nuclear translocation. Finally, α-humulene upregulated the expression levels of mucin 5AC (Muc5ac), Muc6, trefoil factor 1 (Tff1), trefoil factor 2 (Tff2), and polymeric immunoglobulin receptor (pigr). α-Humulene may attenuate HCl/ethanol-induced gastritis by inhibiting histamine release and NF-κB activation and stimulating antioxidants and mucosal protective factors, particularly Muc5ac and Muc6. Therefore, these data suggest that α-humulene is a potential drug candidate for the treatment of stress-induced or alcoholic gastritis. |
| first_indexed | 2024-03-10T11:32:13Z |
| format | Article |
| id | doaj.art-e95ef54f81034830b63c8365539b2261 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T11:32:13Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-e95ef54f81034830b63c8365539b22612023-11-21T19:10:49ZengMDPI AGAntioxidants2076-39212021-05-0110576110.3390/antiox10050761Humulene Inhibits Acute Gastric Mucosal Injury by Enhancing Mucosal IntegrityDahee Yeo0Su-Jung Hwang1Ye-Seul Song2Hyo-Jong Lee3Institute of Pharmaceutical Sciences and Research, College of Pharmacy and Inje, Inje University, 607 Obang-dong, Gimhae 621749, KoreaInstitute of Pharmaceutical Sciences and Research, College of Pharmacy and Inje, Inje University, 607 Obang-dong, Gimhae 621749, KoreaSchool of Pharmacy, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 16419, KoreaInstitute of Pharmaceutical Sciences and Research, College of Pharmacy and Inje, Inje University, 607 Obang-dong, Gimhae 621749, KoreaThis study was designed to determine whether α-humulene, a major constituent in many plants used in fragrances, has a protective role against gastric injury in vivo and in vitro. A rat model of hydrochloric acid (HCl)/ethanol-induced gastritis and human mast cells (HMC-1) were used to investigate the mucosal protective effect of α-humulene. α-Humulene significantly inhibited gastric lesions in HCl/ethanol-induced acute gastritis and decreased gastric acid secretion pyloric ligation-induced gastric ulcers in vivo. In addition, α-humulene reduced the amount of reactive oxygen species and malondialdehyde through upregulation of prostaglandin E2 (PGE2) and superoxide dismutase (SOD). In HMC-1 cells, α-humulene decreased intracellular calcium and increased intracellular cyclic adenosine monophosphate (cAMP) levels, resulting in low histamine levels. α-Humulene also reduced the expression levels of cytokine genes such as interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF) by downregulating nuclear factor-κB (NF-κB) nuclear translocation. Finally, α-humulene upregulated the expression levels of mucin 5AC (Muc5ac), Muc6, trefoil factor 1 (Tff1), trefoil factor 2 (Tff2), and polymeric immunoglobulin receptor (pigr). α-Humulene may attenuate HCl/ethanol-induced gastritis by inhibiting histamine release and NF-κB activation and stimulating antioxidants and mucosal protective factors, particularly Muc5ac and Muc6. Therefore, these data suggest that α-humulene is a potential drug candidate for the treatment of stress-induced or alcoholic gastritis.https://www.mdpi.com/2076-3921/10/5/761α-humulenegastritishistamineantioxidantcytokinemucins |
| spellingShingle | Dahee Yeo Su-Jung Hwang Ye-Seul Song Hyo-Jong Lee Humulene Inhibits Acute Gastric Mucosal Injury by Enhancing Mucosal Integrity Antioxidants α-humulene gastritis histamine antioxidant cytokine mucins |
| title | Humulene Inhibits Acute Gastric Mucosal Injury by Enhancing Mucosal Integrity |
| title_full | Humulene Inhibits Acute Gastric Mucosal Injury by Enhancing Mucosal Integrity |
| title_fullStr | Humulene Inhibits Acute Gastric Mucosal Injury by Enhancing Mucosal Integrity |
| title_full_unstemmed | Humulene Inhibits Acute Gastric Mucosal Injury by Enhancing Mucosal Integrity |
| title_short | Humulene Inhibits Acute Gastric Mucosal Injury by Enhancing Mucosal Integrity |
| title_sort | humulene inhibits acute gastric mucosal injury by enhancing mucosal integrity |
| topic | α-humulene gastritis histamine antioxidant cytokine mucins |
| url | https://www.mdpi.com/2076-3921/10/5/761 |
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