High mannoronic acid containing alginate affects the differentiation of Wharton's jelly-derived stem cells to hepatocyte-like cell
For transplantation of cell into injured tissues, cells should be transferred to the damaged site through an adequate carrier. Nevertheless, the nutrient-limited and hypoxic condition in the carrier can bring about broad cell death. This study set to assess the impact of alginate concentrations on t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Journal of Advanced Pharmaceutical Technology & Research |
| Subjects: | |
| Online Access: | http://www.japtr.org/article.asp?issn=2231-4040;year=2019;volume=10;issue=1;spage=9;epage=15;aulast=Azandeh |
| _version_ | 1831548267516657664 |
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| author | Saeed Azandeh Darioush Bijan Nejad Vahid Bayati Foroug Shakoor Negar Varaa Bahman Cheraghian |
| author_facet | Saeed Azandeh Darioush Bijan Nejad Vahid Bayati Foroug Shakoor Negar Varaa Bahman Cheraghian |
| author_sort | Saeed Azandeh |
| collection | DOAJ |
| description | For transplantation of cell into injured tissues, cells should be transferred to the damaged site through an adequate carrier. Nevertheless, the nutrient-limited and hypoxic condition in the carrier can bring about broad cell death. This study set to assess the impact of alginate concentrations on the differentiation and the proliferation of cells encapsulated in alginate hydrogels. Human Wharton's Jelly-derived Mesenchymal Stem Cells (HWJ-MSCs) were encapsulated in two concentrations of alginate hydrogel. Then, the proliferation and the hepatic differentiation were evaluated with an MTT assay and the enzyme-linked immunosorbent assay software and urea production. The results demonstrated that the proliferation of cell and urea production in 1.5% alginate concentration was higher than in 2.5% alginate concentration in the hydrogels of alginate. We deduce that the optimized alginate hydrogel concentration is necessary for achieving comparable cell activities in three-dimensional culture. |
| first_indexed | 2024-12-17T02:15:58Z |
| format | Article |
| id | doaj.art-e963ef3c6f9f4622adbf72d26c4a6181 |
| institution | Directory Open Access Journal |
| issn | 2231-4040 0976-2094 |
| language | English |
| last_indexed | 2024-12-17T02:15:58Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Advanced Pharmaceutical Technology & Research |
| spelling | doaj.art-e963ef3c6f9f4622adbf72d26c4a61812022-12-21T22:07:24ZengWolters Kluwer Medknow PublicationsJournal of Advanced Pharmaceutical Technology & Research2231-40400976-20942019-01-0110191510.4103/japtr.JAPTR_312_18High mannoronic acid containing alginate affects the differentiation of Wharton's jelly-derived stem cells to hepatocyte-like cellSaeed AzandehDarioush Bijan NejadVahid BayatiForoug ShakoorNegar VaraaBahman CheraghianFor transplantation of cell into injured tissues, cells should be transferred to the damaged site through an adequate carrier. Nevertheless, the nutrient-limited and hypoxic condition in the carrier can bring about broad cell death. This study set to assess the impact of alginate concentrations on the differentiation and the proliferation of cells encapsulated in alginate hydrogels. Human Wharton's Jelly-derived Mesenchymal Stem Cells (HWJ-MSCs) were encapsulated in two concentrations of alginate hydrogel. Then, the proliferation and the hepatic differentiation were evaluated with an MTT assay and the enzyme-linked immunosorbent assay software and urea production. The results demonstrated that the proliferation of cell and urea production in 1.5% alginate concentration was higher than in 2.5% alginate concentration in the hydrogels of alginate. We deduce that the optimized alginate hydrogel concentration is necessary for achieving comparable cell activities in three-dimensional culture.http://www.japtr.org/article.asp?issn=2231-4040;year=2019;volume=10;issue=1;spage=9;epage=15;aulast=AzandehAlginatecell encapsulationhepatic differentiationmesenchymal stem cellsumbilical cord |
| spellingShingle | Saeed Azandeh Darioush Bijan Nejad Vahid Bayati Foroug Shakoor Negar Varaa Bahman Cheraghian High mannoronic acid containing alginate affects the differentiation of Wharton's jelly-derived stem cells to hepatocyte-like cell Journal of Advanced Pharmaceutical Technology & Research Alginate cell encapsulation hepatic differentiation mesenchymal stem cells umbilical cord |
| title | High mannoronic acid containing alginate affects the differentiation of Wharton's jelly-derived stem cells to hepatocyte-like cell |
| title_full | High mannoronic acid containing alginate affects the differentiation of Wharton's jelly-derived stem cells to hepatocyte-like cell |
| title_fullStr | High mannoronic acid containing alginate affects the differentiation of Wharton's jelly-derived stem cells to hepatocyte-like cell |
| title_full_unstemmed | High mannoronic acid containing alginate affects the differentiation of Wharton's jelly-derived stem cells to hepatocyte-like cell |
| title_short | High mannoronic acid containing alginate affects the differentiation of Wharton's jelly-derived stem cells to hepatocyte-like cell |
| title_sort | high mannoronic acid containing alginate affects the differentiation of wharton s jelly derived stem cells to hepatocyte like cell |
| topic | Alginate cell encapsulation hepatic differentiation mesenchymal stem cells umbilical cord |
| url | http://www.japtr.org/article.asp?issn=2231-4040;year=2019;volume=10;issue=1;spage=9;epage=15;aulast=Azandeh |
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