Relationship of transcriptional markers to Leydig cell number in the mouse testis.
<h4>Objectives</h4>The current study aims to identify markers that would reflect the number of Leydig cells present in the testis, to help determine whether labour-intensive methods such as stereology are necessary. We used our well-characterised Sertoli cell ablation model in which we h...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0219524 |
| _version_ | 1818727005484482560 |
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| author | Diane Rebourcet Ana Monteiro Lyndsey Cruickshanks Nathan Jeffery Sarah Smith Laura Milne Peter J O'Shaughnessy Lee B Smith |
| author_facet | Diane Rebourcet Ana Monteiro Lyndsey Cruickshanks Nathan Jeffery Sarah Smith Laura Milne Peter J O'Shaughnessy Lee B Smith |
| author_sort | Diane Rebourcet |
| collection | DOAJ |
| description | <h4>Objectives</h4>The current study aims to identify markers that would reflect the number of Leydig cells present in the testis, to help determine whether labour-intensive methods such as stereology are necessary. We used our well-characterised Sertoli cell ablation model in which we have empirically established the size of the Leydig cell population, to try to identify transcriptional biomarkers indicative of population size.<h4>Results</h4>Following characterisation of the Leydig cell population after Sertoli cell ablation in neonatal life or adulthood, we identified Hsd3b1 transcript levels as a potential indicator of Leydig cell number with utility for informing decision-making on whether to engage in time-consuming stereological cell counting analysis. |
| first_indexed | 2024-12-17T22:07:13Z |
| format | Article |
| id | doaj.art-e9650f18fd44449583ca01c5a02eae31 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T22:07:13Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e9650f18fd44449583ca01c5a02eae312022-12-21T21:30:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01147e021952410.1371/journal.pone.0219524Relationship of transcriptional markers to Leydig cell number in the mouse testis.Diane RebourcetAna MonteiroLyndsey CruickshanksNathan JefferySarah SmithLaura MilnePeter J O'ShaughnessyLee B Smith<h4>Objectives</h4>The current study aims to identify markers that would reflect the number of Leydig cells present in the testis, to help determine whether labour-intensive methods such as stereology are necessary. We used our well-characterised Sertoli cell ablation model in which we have empirically established the size of the Leydig cell population, to try to identify transcriptional biomarkers indicative of population size.<h4>Results</h4>Following characterisation of the Leydig cell population after Sertoli cell ablation in neonatal life or adulthood, we identified Hsd3b1 transcript levels as a potential indicator of Leydig cell number with utility for informing decision-making on whether to engage in time-consuming stereological cell counting analysis.https://doi.org/10.1371/journal.pone.0219524 |
| spellingShingle | Diane Rebourcet Ana Monteiro Lyndsey Cruickshanks Nathan Jeffery Sarah Smith Laura Milne Peter J O'Shaughnessy Lee B Smith Relationship of transcriptional markers to Leydig cell number in the mouse testis. PLoS ONE |
| title | Relationship of transcriptional markers to Leydig cell number in the mouse testis. |
| title_full | Relationship of transcriptional markers to Leydig cell number in the mouse testis. |
| title_fullStr | Relationship of transcriptional markers to Leydig cell number in the mouse testis. |
| title_full_unstemmed | Relationship of transcriptional markers to Leydig cell number in the mouse testis. |
| title_short | Relationship of transcriptional markers to Leydig cell number in the mouse testis. |
| title_sort | relationship of transcriptional markers to leydig cell number in the mouse testis |
| url | https://doi.org/10.1371/journal.pone.0219524 |
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