Propylene glycol – a useful capture preservative for spiders for DNA barcoding
The usefulness of propylene glycol as capture preservative in pitfall traps, with the aim of using the captured spiders for DNA barcoding, was tested. For this purpose a laboratory experiment on the conserving and/or denaturing effect of propylene glycol on mitochondrial DNA (COI) was set up. For th...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | deu |
| Published: |
Arachnologische Gesellschaft (ARAGES)
2015-11-01
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| Series: | Arachnologische Mitteilungen |
| Subjects: | |
| Online Access: |
http://www.arages.de/aramit/pdf/Heft_50/AM50_30_36.pdf
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| _version_ | 1811306161499013120 |
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| author | Höfer, Hubert Astrin, Jonas Holstein, Joachim Spelda, Jörg Meyer, Franziska Zarte, Natalie |
| author_facet | Höfer, Hubert Astrin, Jonas Holstein, Joachim Spelda, Jörg Meyer, Franziska Zarte, Natalie |
| author_sort | Höfer, Hubert |
| collection | DOAJ |
| description | The usefulness of propylene glycol as capture preservative in pitfall traps, with the aim of using the captured spiders for DNA barcoding, was tested. For this purpose a laboratory experiment on the conserving and/or denaturing effect of propylene glycol on mitochondrial DNA (COI) was set up. For the experiment 110 specimens of the common and abundant wolf spider species Pardosa lugubris were manually captured, killed and incubated from one to four weeks in either pure or watered propylene glycol or 70 % denatured ethanol. Rates of successful sequencing, following a standard protocol, did not differ between samples incubated in propylene glycol and in the more commonly used ethanol. Thus, within four weeks, propylene glycol did not significantly denaturize mitochondrial DNA. In two field studies, pitfall traps with propylene glycol captured more spiders than traps with acetic acid. The effect was significant only in one of two field trials, but then consistent at three different sites and the three dominant spider families. Based on these results and our operating experience, we recommend propylene glycol as a capture preservative for (pitfall) traps to obtain specimens for DNA barcoding identification. |
| first_indexed | 2024-04-13T08:40:31Z |
| format | Article |
| id | doaj.art-e96635c800ea489a94b865c8dad2b2ac |
| institution | Directory Open Access Journal |
| issn | 1018-4171 2199-7233 |
| language | deu |
| last_indexed | 2024-04-13T08:40:31Z |
| publishDate | 2015-11-01 |
| publisher | Arachnologische Gesellschaft (ARAGES) |
| record_format | Article |
| series | Arachnologische Mitteilungen |
| spelling | doaj.art-e96635c800ea489a94b865c8dad2b2ac2022-12-22T02:53:56ZdeuArachnologische Gesellschaft (ARAGES)Arachnologische Mitteilungen1018-41712199-72332015-11-0150303610.5431/aramit5005732Propylene glycol – a useful capture preservative for spiders for DNA barcodingHöfer, HubertAstrin, JonasHolstein, JoachimSpelda, JörgMeyer, FranziskaZarte, NatalieThe usefulness of propylene glycol as capture preservative in pitfall traps, with the aim of using the captured spiders for DNA barcoding, was tested. For this purpose a laboratory experiment on the conserving and/or denaturing effect of propylene glycol on mitochondrial DNA (COI) was set up. For the experiment 110 specimens of the common and abundant wolf spider species Pardosa lugubris were manually captured, killed and incubated from one to four weeks in either pure or watered propylene glycol or 70 % denatured ethanol. Rates of successful sequencing, following a standard protocol, did not differ between samples incubated in propylene glycol and in the more commonly used ethanol. Thus, within four weeks, propylene glycol did not significantly denaturize mitochondrial DNA. In two field studies, pitfall traps with propylene glycol captured more spiders than traps with acetic acid. The effect was significant only in one of two field trials, but then consistent at three different sites and the three dominant spider families. Based on these results and our operating experience, we recommend propylene glycol as a capture preservative for (pitfall) traps to obtain specimens for DNA barcoding identification. http://www.arages.de/aramit/pdf/Heft_50/AM50_30_36.pdf acetic acidcapture efficiencyDNA preservationGBOLGerman Barcode of Lifemethodspitfall traps |
| spellingShingle | Höfer, Hubert Astrin, Jonas Holstein, Joachim Spelda, Jörg Meyer, Franziska Zarte, Natalie Propylene glycol – a useful capture preservative for spiders for DNA barcoding Arachnologische Mitteilungen acetic acid capture efficiency DNA preservation GBOL German Barcode of Life methods pitfall traps |
| title | Propylene glycol – a useful capture preservative for spiders for DNA barcoding |
| title_full | Propylene glycol – a useful capture preservative for spiders for DNA barcoding |
| title_fullStr | Propylene glycol – a useful capture preservative for spiders for DNA barcoding |
| title_full_unstemmed | Propylene glycol – a useful capture preservative for spiders for DNA barcoding |
| title_short | Propylene glycol – a useful capture preservative for spiders for DNA barcoding |
| title_sort | propylene glycol a useful capture preservative for spiders for dna barcoding |
| topic | acetic acid capture efficiency DNA preservation GBOL German Barcode of Life methods pitfall traps |
| url |
http://www.arages.de/aramit/pdf/Heft_50/AM50_30_36.pdf
|
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