Association between human papillomavirus (HPV) DNA and micronuclei in normal cervical cytology
The aim of the present study was to investigate the association between HPV-DNA and micronucleus (MN) frequency in women with normal cervical cytology. A total of 158 normal cervical smears were analyzed cytologically. The HPV genome was amplified using the GP5+/bioGP6+ consensus primers. HPV-DNA of...
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| Format: | Article |
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Sociedade Brasileira de Genética
2014-06-01
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| Series: | Genetics and Molecular Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300007&lng=en&tlng=en |
| _version_ | 1818351698962284544 |
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| author | Ana Paula Rebelo Cassel Regina Bones Barcellos Cláudia Maria Dornelles da Silva Sabrina Esteves de Matos Almeida Maria Lucia Rosa Rossetti |
| author_facet | Ana Paula Rebelo Cassel Regina Bones Barcellos Cláudia Maria Dornelles da Silva Sabrina Esteves de Matos Almeida Maria Lucia Rosa Rossetti |
| author_sort | Ana Paula Rebelo Cassel |
| collection | DOAJ |
| description | The aim of the present study was to investigate the association between HPV-DNA and micronucleus (MN) frequency in women with normal cervical cytology. A total of 158 normal cervical smears were analyzed cytologically. The HPV genome was amplified using the GP5+/bioGP6+ consensus primers. HPV-DNA of high-risk types 16, 18, 31, 33, 39, 45 and 59 were also investigated. Of the 158 samples, 20 (12.7%) and 47 (29.7%) were positive for HPV-DNA and MN, respectively. Evidence for MN was found in 11 out of 20 (55%) HPV-DNA positive samples and in 36 out of 138 (26.1%) HPV-DNA negative ones. MN presence was significantly higher in HPV-DNA positive samples (p = 0.016). On the other hand, the absence of MN observed in a considerable number of HPV-DNA negative samples (102) may be of great value in predicting the absence of HPV. The mean age of HPV-DNA positive women (34.2 ± 12.6) was significantly lower than the mean age of HPV-DNA negative women (43.9 ± 13.7) (p = 0.003). Infection by one or multiple HPV types was found in 11 out of 20 (55.0%) and 9 out of 20 (45.0%) samples, respectively. The evaluation of MN using cervical smears collected for cytology tests could, thus, be used as additional information to monitor a population's exposure to HPV. |
| first_indexed | 2024-12-13T18:41:53Z |
| format | Article |
| id | doaj.art-e96699e852fb4312b15a01f44187924e |
| institution | Directory Open Access Journal |
| issn | 1678-4685 |
| language | English |
| last_indexed | 2024-12-13T18:41:53Z |
| publishDate | 2014-06-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-e96699e852fb4312b15a01f44187924e2022-12-21T23:35:12ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-46852014-06-0137236036310.1590/S1415-47572014000300007S1415-47572014000300007Association between human papillomavirus (HPV) DNA and micronuclei in normal cervical cytologyAna Paula Rebelo Cassel0Regina Bones Barcellos1Cláudia Maria Dornelles da Silva2Sabrina Esteves de Matos Almeida3Maria Lucia Rosa Rossetti4Universidade Luterana do BrasilFundação Estadual de Produção e Pesquisa em SaúdeFundação Estadual de Produção e Pesquisa em SaúdeFundação Estadual de Produção e Pesquisa em SaúdeUniversidade Luterana do BrasilThe aim of the present study was to investigate the association between HPV-DNA and micronucleus (MN) frequency in women with normal cervical cytology. A total of 158 normal cervical smears were analyzed cytologically. The HPV genome was amplified using the GP5+/bioGP6+ consensus primers. HPV-DNA of high-risk types 16, 18, 31, 33, 39, 45 and 59 were also investigated. Of the 158 samples, 20 (12.7%) and 47 (29.7%) were positive for HPV-DNA and MN, respectively. Evidence for MN was found in 11 out of 20 (55%) HPV-DNA positive samples and in 36 out of 138 (26.1%) HPV-DNA negative ones. MN presence was significantly higher in HPV-DNA positive samples (p = 0.016). On the other hand, the absence of MN observed in a considerable number of HPV-DNA negative samples (102) may be of great value in predicting the absence of HPV. The mean age of HPV-DNA positive women (34.2 ± 12.6) was significantly lower than the mean age of HPV-DNA negative women (43.9 ± 13.7) (p = 0.003). Infection by one or multiple HPV types was found in 11 out of 20 (55.0%) and 9 out of 20 (45.0%) samples, respectively. The evaluation of MN using cervical smears collected for cytology tests could, thus, be used as additional information to monitor a population's exposure to HPV.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300007&lng=en&tlng=enhuman papillomavirusmicronucleuscytologyhigh-risk HPVSouthern Brazil |
| spellingShingle | Ana Paula Rebelo Cassel Regina Bones Barcellos Cláudia Maria Dornelles da Silva Sabrina Esteves de Matos Almeida Maria Lucia Rosa Rossetti Association between human papillomavirus (HPV) DNA and micronuclei in normal cervical cytology Genetics and Molecular Biology human papillomavirus micronucleus cytology high-risk HPV Southern Brazil |
| title | Association between human papillomavirus (HPV) DNA and micronuclei in normal cervical cytology |
| title_full | Association between human papillomavirus (HPV) DNA and micronuclei in normal cervical cytology |
| title_fullStr | Association between human papillomavirus (HPV) DNA and micronuclei in normal cervical cytology |
| title_full_unstemmed | Association between human papillomavirus (HPV) DNA and micronuclei in normal cervical cytology |
| title_short | Association between human papillomavirus (HPV) DNA and micronuclei in normal cervical cytology |
| title_sort | association between human papillomavirus hpv dna and micronuclei in normal cervical cytology |
| topic | human papillomavirus micronucleus cytology high-risk HPV Southern Brazil |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300007&lng=en&tlng=en |
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